Transdentinal photobiostimulation of stem cells from human exfoliated primary teeth

Abstract

Infrared LED irradiation was capable of biostimulating SHEDs through a 0.2 mm thickness of dentine, especially at the 4 J cm level.

“Metabolism of Odontoblast-like cells submitted to transdentinal irradiation with blue and red LED”

“…Moreover, red light is known to present less scattering and losses during its trajectory when compared with the blue light (Neupane et al, 2010). It can be suggested that during cell exposure to the blue LED, the light dissipation impaired reaching target cells effectively (Turrioni et al, 2016). Another parameter considered was the temperature change by light exposition.…”

“…A total of 78 human third molars were used after the approval from the Research Ethics Committee (CAAE 13072813.2.0000.5416) of the Araraquara School of Dentistry − UNESP. The teeth were cut and ground (Turrioni et al, 2016) to obtain dentin discs with a final thickness of 0.2 mm. The smear layer was removed by applying an EDTA 0.5 M (pH 7.2) solution for 30 seconds on both surfaces of the discs, and subsequent deionized water rinsing for 60 seconds.…”

Photobiomodulation effect of red LED (630 nm) on the free radical levels produced by pulp cells under stress conditions

“…The irradiation of cultured pulp cells was performed using a LED table; this device is widely employed in laboratorial studies in this research field (Fig. 1) [2,22,23,[39][40][41]. The LED table was developed by the Optical Group (OG) of the Optics and Photonics Research Center (Centro de Pesquisa em Óptica e Fotônica -CePOF) of the São Carlos Institute of Physics (IFSC) at the University of São Paulo (USP).…”

“…The potential beneficial parameters (4 J/cm 2 , 15 J/cm 2 , and 30 J/cm 2 at 0.04 W/cm 2 ) [20,23,30,39,40], as well as more intense parameters, which may be considered to be potentially harmful to the cells (4 J/cm 2 , 15 J/cm 2 , and 30 J/cm 2 at 0.08 W/cm 2 ), were assessed. The irradiation parameters used in this study are shown in Tables 2 and 3.…”

Desenvolvimento e caracterização de equivalentes dérmicos associados a fármaco fotossensibilizante para estudos de fotoprocessos

“…Na tabela abaixo foram reunidos os principais substitutos cutâneos encontrados na literatura. , 2016a;DOURADO et al, 2015;FARFARA et al, 2015;TURRIONI et al, 2017;AHMED e SAYED, 2012;DAWOOD e SALMAN, 2013;NOVAES et al, 2014;MARTINS et al, 2017;ELSON e FORAN, 2015). Além disso, estudos apontam que esta modalidade possui efeitos celulares na proliferação de fibroblastos, matriz extracelular, produção de colágeno, formação do tecido de granulação e redução da resposta inflamatória, tornando-se uma terapia alternativa para os processos de cicatrização e reparação tecidual (DEYHIMI et al, 2016 Lubart e colaboradores estudaram a hipótese de que a fotobioestimulação poderia ser mediada pela produção de pequenas quantidades de espécies reativas de oxigênio e para tal, concentrações baixas de derivados de hematoporfirina foram adicionados às culturas de fibroblastos que foram irradiadas com laser de HeNe.…”