Transdentinal Cell Photobiomodulation Using Different Wavelengths

Turrioni, Ana Paula Silveira; Basso, Fernanda Gonçalves; Alonso, Juliana Rosa Luiz; Oliveira, Carlos; Hebling, Josimeri; Bagnato, Vanderlei Salvador; Costa, Carlos Alberto de Souza

doi:10.2341/13-370-l

Cited by 17 publications

(49 citation statements)

References 38 publications

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“…Based on the analysis of positive data provided by previous studies, researchers have been encouraged to develop more investigations to establish the most beneficial physical parameters of LED or laser irradiation of pulp cells (8)(9)(10)(11)20).…”

Section: Discussionmentioning

confidence: 99%

“…For cell irradiation, a previously described standardized device (LEDtable) containing 24 infrared diodes (850 nm) was used (9)(10)(11). The irradiance was fixed at 40 mW/cm 2 , and the irradiation times were 50 s (2 J/cm 2 ), 1 min and 40 s (4 J/cm 2 ), 3 min and 20 s (8 J/cm 2 ), 6 min and 15 s (15 J/cm 2 ) and 12 min and 30 s (30 J/cm 2 ) (9).…”

Section: Irradiation Devicementioning

confidence: 99%

“…Low-level light therapy (LLLT), with lasers or light-emitting diodes (LED), has been widely used in dentistry for tissue and cell stimulation (5)(6)(7)(8)(9)(10)(11). Concerning the pulp tissue or pulp cells subjected to phototherapy, several authors have reported encouraging scientific data, such as increased deposition of tertiary dentin (5), enhanced cell viability and proliferation (8,11), and biomodulation of inflammatory cytokines and reactive oxygen species (9). Despite these positive results, a major challenge in tissue biostimulation involving phototherapy is to define the optimal window for each cell type and desired action (3).…”

Section: Introductionmentioning

confidence: 99%

“…The authors reported that the smallest scattering of light occurred when infrared LED was used. Later, it was shown that the light emitted by diodes is able not only to cross the dentin barrier but also to stimulate the underlying pulp cells (11).…”

Section: Introductionmentioning

confidence: 99%

“…Despite the promising cell-biostimulatory results obtained with phototherapy, such as modulation of tissue inflammation (15) and stimulation of fibroblast cell metabolism, odontoblast-like cells, and human pulp cells (10,11,(16)(17)(18), information about the effects of infrared LED on the metabolism and mineralization ability of stem cells from human exfoliated deciduous teeth is scant. Therefore, the aim of this study was to evaluate the effects of 850-nm LED irradiation on cultured stem cells from human exfoliated deciduous teeth (SHEDs) at different energy densities.…”

Section: Introductionmentioning

confidence: 99%

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Dose-responses of Stem Cells from Human Exfoliated Teeth to Infrared LED Irradiation

et al. 2015

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Despite several reports regarding tissue regeneration, including pulp repair induced by different light sources, only limited data have been reported concerning the effects of light-emitting diodes (LED) on stem cells from human exfoliated deciduous teeth (SHEDs). The aim of this study was to evaluate the effects of different energy densities of infrared LED on the cell viability, number of cells and mineralized tissue production by SHEDs. SHEDs were obtained from near-exfoliation primary teeth (n=3), seeded in plain DMEM (10 4 cells/cm 2 ), and irradiated by a LED prototype (LEDTable 850 nm, 40 mW/cm 2 ) delivering 0 (control), 2, 4, 8, 15 or 30 J/cm 2 (n=9). Cell viability (MTT assay), cell proliferation (trypan blue assay), and mineralized nodule (MN) formation (alizarin red stain) were assessed 12 and 72 h post-irradiation. Data were subjected to Kruskal-Wallis and Mann-Whitney tests (α=0.05). Cells irradiated with 2 or 4 J/cm 2 exhibited higher metabolism at 72 h, and all energy densities provided increase in cell proliferation after 12 h. Regarding MN formation, the best results were observed at 72 h after SHED irradiation with 8 and 15 J/cm 2 . It was concluded that the cell viability, cell number and MN formation by pulp cells are enhanced after exposure to infrared LED irradiation. Overall, the greatest SHED biostimulation was obtained with 4 and 8 J/cm 2 . D o s e -r e s p o n s e s o f S t e m C e l l s f r o m H u m a n E x f o l i a t e d Te e t h t o I n f r a r e d L E D I r r a d i a t i o n

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Section: Discussionmentioning

confidence: 99%

Section: Irradiation Devicementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Dose-responses of Stem Cells from Human Exfoliated Teeth to Infrared LED Irradiation

et al. 2015

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Three-dimensional imaging and molecular analysis of the effects of photobiomodulation and mechanical vibration on orthodontic retention treatment in rats

Öztürk

Amuk

2021

J Orofac Orthop

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Synthesis of dental matrix proteins and viability of odontoblast-like cells irradiated with blue LED

et al. 2016

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To evaluate the effect of irradiation with light-emitting diode (LED; 455 nm) on the viability and synthesis of dentin matrix proteins by odontoblast-like cells, MDPC-23 cells were cultivated (10(4) cells/cm(2)) in 24-well culture plates. After 12 h incubation in Dulbecco's modified Eagle's medium (DMEM), the cells were submitted to nutritional restriction by means of reducing the concentration of fetal bovine serum (FBS) for an additional 12 h. Cells were irradiated one single time with one of the following energy densities (EDs): 0.5, 2, 4, 10, or 15 J/cm(2) and irradiance fixed at 20 mW/cm(2). Non-irradiated cells served as control. After 72 h, cells were evaluated with regard to viability (methylthiazol tetrazolium technique (MTT)), mineralization nodule (MN) formation, total protein (TP) production, alkaline phosphatase activity (ALP), and collagen synthesis (Sircol), n = 8. The data were submitted to Kruskal-Wallis and Mann-Whitney tests (p > 0.05). There was no statistical difference between the viability of cells irradiated or not (control), for all the EDs. However, an increase in TP was observed for all the EDs when compared with the control group. A reduced ALP activity was seen in all irradiated groups, except for the ED of 0.5 J/cm(2), which did not differ from the control. There was no difference between the irradiated groups and control regarding collagen synthesis, with the exception of the ED of 10 J/cm(2), which inhibited this cell function. Significant reduction in MN occurred only for the EDs of 0.5 and 2 J/cm(2). The single irradiation with blue LED (455 nm), irradiance of 20 mW/cm(2), and energy densities ranging from 0.5 to 15 J/cm(2) exerted no effective biostimulatory capacity on odontoblast-like cells.

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Transdentinal Cell Photobiomodulation Using Different Wavelengths

Abstract: The infrared LED irradiation at an energy density of 4 J/cm(2) and red LED at an energy density of 25 J/cm(2) were the most effective parameters for transdentinal photobiomodulation of cultured odontoblast-like cells.

Cited by 17 publications

References 38 publications

Dose-responses of Stem Cells from Human Exfoliated Teeth to Infrared LED Irradiation

Dose-responses of Stem Cells from Human Exfoliated Teeth to Infrared LED Irradiation

Three-dimensional imaging and molecular analysis of the effects of photobiomodulation and mechanical vibration on orthodontic retention treatment in rats

Synthesis of dental matrix proteins and viability of odontoblast-like cells irradiated with blue LED

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