Transcytotic vesicle fusion with canalicular membrane was reduced in cholestatic rats: relation to redistribution of phospholipid molecular species within membrane bilayer

Hyogo, Hideyuki; Tazuma, Susumu; Gunji, Yoshio; Miura, H.; Kajihara, Toshiki; Miyake, Hiroaki; Yasumiba, Shigeyuki; Sumami, Y.; Hattori, Yuya; Nishioka, T.; Kajiyama, Goro

doi:10.1016/s0016-5085(98)85109-3

Cited by 7 publications

(11 citation statements)

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“…These data are in accordance with those obtained in cultured cells and tissues (1,5,16,29). The increase of membrane fluidity can also be explained by a change in fatty acid lipid composition that causes an increase in free volume between the lipid chains, due to the presence of double bounds in fatty acids (18:2 and 20:4), leading to a bend in the lipid chain itself.…”

Section: Discussionsupporting

confidence: 90%

Composition, biophysical properties, and morphometry of plasma membranes in pulmonary interstitial edema

Palestini

Calvi

Conforti

et al. 2002

American Journal of Physiology-Lung Cellular and Molecular Phys

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. Composition, biophysical properties, and morphometry of plasma membranes in pulmonary interstitial edema. Am J Physiol Lung Cell Mol Physiol 282: L1382-L1390, 2002. First published February 1, 2002 10.1152/ajplung.00447.2001.-We evaluated the changes in plasma membrane composition, biophysical properties, and morphology of pulmonary endothelial cells in anesthetized rabbits receiving 0.5 ml ⅐ kg Ϫ1 ⅐ min Ϫ1 saline infusion for 180 min, causing mild interstitial edema. Plasma membrane fractions were obtained from lung homogenates with gradient centrifugation, allowing a sixfold enrichment in caveolin-1. In edematous lungs, cholesterol content and phospholipidic phosphorus increased by 15 and 40%, respectively. These data correlated with morphometric analysis of lungs fixed in situ by vascular perfusion with 2.5% glutaraldehyde, suggesting a relative increase in surface of luminal to interstitial front of the capillary endothelial cells, due to a convoluted luminal profile. In edematous lungs, the fraction of double-bound fatty acids increased in membrane lipids; moreover, the phosphatidylcholine/phosphatidylethanolamine and the cholesterol/phospholipid ratios decreased. These changes were consistent with the increase in fluorescence anisotropy of plasma membrane, indicating an increase in its fluidity. Data suggest that mechanical stimuli elicited by a modest (ϳ4%) increase in extravascular water cause marked changes in plasma membranes that may be of relevance in signal transduction and endothelial cell activation. rabbit; air-blood barrier; endothelial cells; membrane composition and fluidity THE ALVEOLAR AND CAPILLARY walls delimit the pulmonary interstitium, a thin compartment made of a fiber system, serving as a scaffold, and other macromolecules forming the capillary and the alveolar basement membranes. Extravascular water is distributed within the interstitial compartment of the air-blood barrier and is physiologically kept at a minimum volume to optimize gas diffusion. Recent work, based on an experimental model causing a mild form of pulmonary interstitial edema, allowed us to describe the mechanisms contributing to a tight control of extravascular lung volume in the air-blood barrier (6,20). These studies also showed that, despite a remarkable resistance of the pulmonary tissue to edema formation, the increase in interstitial fluid volume occurs as a consequence of fragmentation of proteoglycans, important macromolecules of the extracellular matrix (24,25,31,32) controlling microvascular permeability and tissue mechanical resistance. We wished to further investigate the condition of mild interstitial edema because it represents a critical equilibrium between matrix degradation and deposition provided by cellular activation. We focused on cellular involvement in mild interstitial edema, particularly on the composition and fluidity of plasma membranes that are known to be sensitive to alterations in chemical and physical stimuli of the environment surrounding the cells (34, 36). MATERIALS AND METHODS C...

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Section: Discussionsupporting

confidence: 90%

Composition, biophysical properties, and morphometry of plasma membranes in pulmonary interstitial edema

Palestini

Calvi

Conforti

et al. 2002

American Journal of Physiology-Lung Cellular and Molecular Phys

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“…In fact, increased cholesterol contents in the canalicular membrane of the cholestatic rats have been reported and the hypothesis of the existence of decreased membrane fluidity has been entertained (Hyogo et al, 1999). This was later confirmed in acute cholestasis induced by selective ligation of some bile duct branches (Kanno et al, 2003).…”

Section: Impaired Bile Secretion In the Offspring Of Cholestatic Ratsmentioning

confidence: 92%

“…Thus, the transfer of biliary lipids from intracellular compartments toward the canalicular membrane is probably also affected. It has been reported that the rates of liposomal fusion to membranes are decreased in cholestatic rats and that a redistribution of phospholipid species within canalicular membranes is associated with decreased transcytotic vesicle fusion (Hyogo et al, 1999). This suggests the existence of alterations in the physical-chemical properties of transcellular/canalicular membranes, which may be involved in both the formation of multilamellar bodies and their intracellular location.…”

Section: Impaired Bile Secretion In the Offspring Of Cholestatic Ratsmentioning

confidence: 99%

Long-Term Effect of Treating Pregnant Rats with Ursodeoxycholic Acid on the Congenital Impairment of Bile Secretion Induced in the Pups by Maternal Cholestasis

Macı́as

Serrano

Monte

et al. 2004

J Pharmacol Exp Ther

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Transient latent cholestasis in young rats born from mothers with obstructive cholestasis during pregnancy (OCP) has been reported. The cause of this congenital impairment and the long-term effect on the pups of treating their mothers with ursodeoxycholic acid (UDCA) during pregnancy were investigated. Complete biliary obstruction was imposed on day 14 of pregnancy and UDCA treatment was begun on day 15. Serum bile acids (BAs) concentrations were elevated in 4-week-old pups born from OCP, but not OCP ϩ UDCA, mothers. However, gas chromatographic/mass spectrometric analysis of BA species in basal bile indicated the presence of significant differences among all experimental groups (control, OCP, and OCP ϩ UDCA). Canalicular plasma membrane fluidity was reduced in OCP, but not in OCP ϩ UDCA, pups. Screening by reverse transcription followed by real-time quantitative polymerase chain reaction of the steady-state levels of mRNA of genes related to hepatobiliary function revealed changes (upregulation of Cyp7a1 and Mrp1 and down-regulation of Abcg5 and Abcg8) in OCP group, which were prevented by UDCA treatment. Electron microscopy examination showed multilamellar bodies occupying part of the canalicular lumen in OCP pups. Their number and size were reduced in animals born from OCP ϩ UDCA mothers. In OCP, but not OCP ϩ UDCA, the stimulation of bile flow and BA output induced by taurocholate administration were reduced and cholesterol/BA output ratio was increased, whereas phospholipid/BA output ratio was enhanced in both groups (OCP Ͼ OCP ϩ UDCA). In conclusion, UDCA treatment of rats with cholestasis during pregnancy has long-term beneficial effects on their offspring by preventing in part the congenital impairment in hepatobiliary function of the pups that affects their biliary lipid secretion.

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“…Increased cholesterol content in the canalicular membrane of cholestatic rats has been reported and the hypothesis of the existence of decreased membrane fluidity has been entertained (Hyogo et al, 1999). This was later confirmed in acute cholestasis induced by selective ligation of some bile duct branches (Kanno et al, 2003).…”

Section: Discussionmentioning

confidence: 90%

Effect of maternal cholestasis and treatment with ursodeoxycholic acid on the expression of genes involved in the secretion of biliary lipids by the neonatal rat liver

et al. 2006

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Transcytotic vesicle fusion with canalicular membrane was reduced in cholestatic rats: relation to redistribution of phospholipid molecular species within membrane bilayer

Cited by 7 publications

References 0 publications

Composition, biophysical properties, and morphometry of plasma membranes in pulmonary interstitial edema

Composition, biophysical properties, and morphometry of plasma membranes in pulmonary interstitial edema

Long-Term Effect of Treating Pregnant Rats with Ursodeoxycholic Acid on the Congenital Impairment of Bile Secretion Induced in the Pups by Maternal Cholestasis

Effect of maternal cholestasis and treatment with ursodeoxycholic acid on the expression of genes involved in the secretion of biliary lipids by the neonatal rat liver

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