Transcutaneous vaccination via laser microporation

Weiss, Richard; Hessenberger, Michael; Kitzmüller, Sophie; Bach, D.; Weinberger, Esther E.; Krautgartner, Wolf‐Dietrich; Hauser‐Kronberger, Cornelia; Malissen, Bernard; Boehler, Christof; Kalia, Yogeshvar N.; Thalhamer, Josef; Scheiblhofer, Sandra

doi:10.1016/j.jconrel.2012.06.031

Cited by 85 publications

(87 citation statements)

References 62 publications

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“…Laser-based systems represent the third strategy of thermal microporation. Originally developed for medical surgery [148], these devices use infrared light at = 2.94 m generated by an Erbium doped yttrium aluminum garnet (Er:YAG) laser [149]. The absorption of the infrared light induces vibrations within the water molecules leading to vaporization [148,150].…”

Section: Thermal Microporation Technologiesmentioning

confidence: 99%

Recent insights into cutaneous immunization: How to vaccinate via the skin

Engelke

Winter

Hook

et al. 2015

Vaccine

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Section: Thermal Microporation Technologiesmentioning

confidence: 99%

Recent insights into cutaneous immunization: How to vaccinate via the skin

Engelke

Winter

Hook

et al. 2015

Vaccine

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“…These approaches generated robust humoral and cellular immune responses. A portable laser, the Precise Laser Epidermal System (P.L.E.A.S.E), also was used to create micropores in the stratum corneum, the superficial impermeable layer of the skin, and the epidermis, allowing topically applied Ags to diffuse into the dermis and to induce potent immune responses (20).…”

mentioning

confidence: 99%

Laser-Assisted Intradermal Delivery of Adjuvant-Free Vaccines Targeting XCR1+ Dendritic Cells Induces Potent Antitumoral Responses

Terhorst‐Molawi

Fossum

Barańska

et al. 2015

The Journal of Immunology

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The development of vaccines inducing efficient CD8+ T cell responses is the focus of intense research. Dendritic cells (DCs) expressing the XCR1 chemokine receptor, also known as CD103+ or CD8α+ DCs, excel in the presentation of extracellular Ags to CD8+ T cells. Because of its high numbers of DCs, including XCR1+ DCs, the skin dermis is an attractive site for vaccine administration. By creating laser-generated micropores through the epidermis, we targeted a model protein Ag fused to XCL1, the ligand of XCR1, to dermal XCR1+ DCs and induced Ag-specific CD8+ and CD4+ T cell responses. Efficient immunization required the emigration of XCR1+ dermal DCs to draining lymph nodes and occurred irrespective of TLR signaling. Moreover, a single intradermal immunization protected mice against melanoma tumor growth in prophylactic and therapeutic settings, in the absence of exogenous adjuvant. The mild inflammatory milieu created in the dermis by skin laser microporation itself most likely favored the development of potent T cell responses in the absence of exogenous adjuvants. The existence of functionally equivalent XCR1+ dermal DCs in humans should permit the translation of laser-assisted intradermal delivery of a tumor-specific vaccine targeting XCR1+ DCs to human cancer immunotherapy. Moreover, considering that the use of adjuvants in vaccines is often associated with safety issues, the possibility of inducing protective responses against melanoma tumor growth independently of the administration of exogenous adjuvants should facilitate the development of safer vaccines.

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“…The infectious flora consist of microorganisms that could cause skin diseases such as abscess, acne, and paronychia (Lange-Asschenfeldt et al, 2011). The skin reepithelialization after ablative fractional laser abrasion was completed within 48 h (Weiss et al, 2012). Nevertheless, the ablative laser elicited a risk of viral and…”

Section: Accepted Manuscriptmentioning

confidence: 99%