Transcutaneous immunization using a dissolving microneedle array protects against tetanus, diphtheria, malaria, and influenza

Matsuo, Keitaro; Hirobe, Sachiko; Yokota, Yoshiko; Ayabe, Yurika; Seto, Masashi; Quan, Ying‐Shu; Kamiyama, Fumio; Tougan, Takahiro; Horii, Toshihiro; Mukai, Yohei; Okada, Naoki; Nakagawa, Shinsaku

doi:10.1016/j.jconrel.2012.04.001

Cited by 125 publications

(54 citation statements)

References 24 publications

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“…Matsuo et al reported that cutaneous immunization of non-pregnant rats with dissolving MNPs containing unadjuvanted TT induced effective immune responses as well as IM and ID routes of TT delivery after 5 vaccine doses [55]. Our delivery system demonstrated superiority to IM and ID routes in both non-pregnant and pregnant cohorts and their offspring.…”

Section: Discussionmentioning

confidence: 74%

Tetanus vaccination with a dissolving microneedle patch confers protective immune responses in pregnancy

Esser

Romanyuk

Vassilieva

et al. 2016

Journal of Controlled Release

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Section: Discussionmentioning

confidence: 74%

Tetanus vaccination with a dissolving microneedle patch confers protective immune responses in pregnancy

Esser

Romanyuk

Vassilieva

et al. 2016

Journal of Controlled Release

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“…Various technologies are also available to transform liquid vaccines, biological agents or drugs into dry forms, like freeze-drying, spray-drying, and spray-freeze-drying. The powder-laden MNA may be applicable to diphtheria, tetanus, malaria [48], anthrax and staphylococcal toxic shock vaccines as well since these vaccines can elicit effective immune responses when delivered by other microneedles [49]. …”

Section: Discussionmentioning

confidence: 99%

BCG vaccine powder-laden and dissolvable microneedle arrays for lesion-free vaccination

Chen¹,

Yan²,

et al. 2017

Journal of Controlled Release

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Live attenuated Bacille Calmette-Guerin (BCG) bacillusis the only licensed vaccine for tuberculosis prevention worldwide to date. It must be delivered intradeemally to be effective, which causes severe skin inflammation and sometimes, permanent scars. To minimize the side effects, we developed a novel microneedle array (MNA) that could deliver live attenuated freeze-dried BCG powder into the epidermis in a painless, lesion-free, and self-applicable fashion. The MNA was fabricated with biocompatible and dissolvable hyaluronic acid with a deep cave formed in the basal portion of each microneedle, into which BCG powder could be packaged directly. Viability of BCG vaccine packaged in the caves and the mechanical strength of the powder-laden MNA did not alter significantly before and after more than two months of storage at room temperature. Following insertion of the MNA into the skin, the individual microneedle shafts melted away by interstitial fluid from the epidermis and upper dermis, exposing the powder to epidermal tissues. The powder sucked interstitial fluid, dissolved slowly, and diffused into the epidermis in a day against the interstitial fluid influx. Vaccination with BCG-MNA caused no overt skin irritation, in marked contrast to intradermal vaccination that provoked severe inflammation and bruise.While causing little skin irritation, vaccination efficacy of BCG-MNAs was comparable to that of intradermal immunization whether it was evaluated by humoral or cellular immunity. This powder-laden and dissolvable MNA represents a novel technology to sufficiently deliver live attenuated vaccine powders into the skin.

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“…Microneedle patches can be applied to the skin in a simple manner, such that microscopic needles painlessly puncture the skin to administer IPV without the need for hypodermic needles [12]. Microneedle patches have previously been used to administer other vaccines in preclinical studies, such as influenza, measles, HPV and others [13][14][15][16][17][18][19][20][21], but have not yet been studied for IPV vaccination.…”

Section: Introductionmentioning

confidence: 99%

Inactivated polio vaccination using a microneedle patch is immunogenic in the rhesus macaque

Edens

Dybdahl-Sissoko

Weldon

et al. 2015

Vaccine

101

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The phased replacement of oral polio vaccine (OPV) with inactivated polio vaccine (IPV) is expected to significantly complicate mass vaccination campaigns, which are an important component of the global polio eradication endgame strategy. To simplify mass vaccination with IPV, we developed microneedle patches that are easy to administer, have a small package size, generate no sharps waste and are inexpensive to manufacture. When administered to rhesus macaques, neutralizing antibody titers were equivalent among monkeys vaccinated using microneedle patches and conventional intramuscular injection for IPV types 1 and 2. Serologic response to IPV type 3 vaccination was weaker after microneedle patch vaccination compared to intramuscular injection; however, we suspect the administered type 3 dose was lower due to a flawed pre-production IPV type 3 analytical method. IPV vaccination using microneedle patches was well tolerated by the monkeys. We conclude that IPV vaccination using a microneedle patch is immunogenic in rhesus macaques and may offer a simpler method of IPV vaccination of people to facilitate polio eradication.

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Transcutaneous immunization using a dissolving microneedle array protects against tetanus, diphtheria, malaria, and influenza

Cited by 125 publications

References 24 publications

Tetanus vaccination with a dissolving microneedle patch confers protective immune responses in pregnancy

Tetanus vaccination with a dissolving microneedle patch confers protective immune responses in pregnancy

BCG vaccine powder-laden and dissolvable microneedle arrays for lesion-free vaccination

Inactivated polio vaccination using a microneedle patch is immunogenic in the rhesus macaque

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