Transcripts of Tla genes

Matsuura, Akihiro; Shen, Francis X.; Fisher, Douglas A.; Hood, Leroy; Boyse, Edward A.

doi:10.1007/bf00396109

Cited by 10 publications

(6 citation statements)

References 12 publications

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“…Full-length cDNA clones containing either intact intron 1 or 2 have been reported for mouse TL class I genes (10,51 (13) and P-globin transcripts (50). A number of nonclassical class I genes of mice and humans have termination codons within internal exons, and an effect of these termination codons in down modulating the levels of gene transcripts in cells could be an important mechanism in limiting the expression of these genes.…”

Section: At:ga C T Ccacga C C T --C --A----------------c --A T -------mentioning

confidence: 99%

Transcription analysis, physical mapping, and molecular characterization of a nonclassical human leukocyte antigen class I gene.

Chorney¹,

Sawada²,

Gillespie³

et al. 1990

Mol. Cell. Biol.

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The human major histocompatibility complex contains approximately 20 class I genes, pseudogenes, and gene fragments. These include the genes for the three major transplantation antigens, HLA-A, HLA-B, and HLA-C, as well as a number of other genes or pseudogenes of unknown biological significance. Most of the latter have C + G-rich sequences in their 5' ends that are unmethylated in the B-lymphoblastoid cell line 3.1.0. We investigated one of these genes, HLA-H, in more detail. The gene is, overall, strongly homologous in sequence to HLA-A but differs in several potentially significant ways, including changes in conserved promoter sequences, a single-base deletion producing a translation termination codon in exon 4, and a region of sequence divergence downstream of the transcribed portion of the gene. Nevertheless, mouse L cells transfected with the gene accumulated small amounts of apparently full-length polyadenylated RNA. A portion of this RNA begins at the transcription site predicted by analogy to certain class I cDNA clones, while another portion appears to begin shortly upstream. L cells transfected with a hybrid gene containing the first three exons of HLA-H and the last five exons of HLA-B27 accumulated full-length HLA transcripts at the same level as cells transfected with an HLA-B27 gene; both levels are at least 15- to 20-fold higher than that directed by HLA-H alone. In addition, we isolated a cDNA clone for HLA-H that contains a portion of intron 3 attached to a normally spliced sequence comprising exons 4 through 8. These results suggest that low levels of translatable mRNA for the truncated class I heavy chain encoded by HLA-H are produced under physiologic circumstances and that sequences 3' of intron 3 decrease the levels of stable transcripts.

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Section: At:ga C T Ccacga C C T --C --A----------------c --A T -------mentioning

confidence: 99%

Transcription analysis, physical mapping, and molecular characterization of a nonclassical human leukocyte antigen class I gene.

Chorney¹,

Sawada²,

Gillespie³

et al. 1990

Mol. Cell. Biol.

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“…High levels of incomplete splicing has also been reported for cDNA clones for other genes (rat MRCOX2 [18]; rat MRCOXS, [19]; Tla [20] isolated from libraries prepared from thymic RNA. However, it is interesting that this is not a phenomenon observed with all genes isolated from thymic cDNA libraries, since cDNA clones for the leukocyte common antigen (CD45) obtained from the same library as those from which MRC OX2 and MRC OX8 were found, were completely normal [21].…”

Section: Discussionmentioning

confidence: 88%

“…Neither is the problem unique to the thymus, since there are many reported examples of occasional unspliced cDNA clones being found in cDNA libraries [22,231. Furthermore,Tcell lines also contain unspliced Tla mRNA and this incomplete splicing is not due to contamination of cytoplasmic RNA with nuclear RNA [20].…”

Section: Discussionmentioning

confidence: 99%

Expression of CD1 in the mouse thymus

1990

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The CD1 antigens are a family of differentiation antigens found predominantly, but not exclusively, in the human thymus. Although three antigens (CD1a-c) are described by monoclonal antibodies, five genes (CD1A-E) are found in the human genome. The cloning of the mouse CD1 genes (Bradbury, A., Belt, K.T., Nery, T.M., Milstein, C. and Calabi, F., EMBO J. 1988. 7:3081) demonstrated the presence of homologues to human CD1D, but not to any of the other human CD1 genes. In this work we have examined the expression of mouse CD1D mRNA in the thymus and shown that it is predominantly cortical, as is the expression of the CD1 antigens in man. Somewhat surprisingly, we also find that most CD1D mRNA in the mouse thymus is unspliced. Despite this, we have also been able to show, using a polyclonal antiserum directed against a bacterial fusion protein, the existence of the expected protein product.

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“…A third type (3/11, type C) represented incompletely spliced mRNA containing intron sequences. All 11 clones lacked exon 7, as is typical of all T/a and Q genes so far sequenced (Chen et al 1985, Waneck et al 1987, Matsuura et al 1987, Robinson et al 1988, Devlin et al 1985, Mellor et al 1984. All three types of mRNA were evidently derived from a single gene because all had identical sequences in the region of exon 5 and in the 3' untranslated (UT) region, both regions being relatively diverse among class I genes.…”

Section: Resultsmentioning

confidence: 96%

“…A cDNA library in the pcD vector (Okayama and Berg 1983), made from the BALB/c (H-2 d) TL-inducer T. i cell clone C1. Ly 1-T 1 (BALB subline of uncertain origin), was screened with the MHC class I probe pH-2-IIa (Steinmetz et al 1981 a) and then with the oligonuleotide probe 5'-GCTCTTCCCCAGAAGGC-3' (probe Tla cEx-4) which is complementary to the exon 4 (Ex-4) sequence of the BALB/c (Tla c) gene T13" (Matsuura et al 1987).…”

Section: Resultsmentioning

confidence: 99%

Expression of theQ8/9 d gene by T cells of the mouse

et al. 1989

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The Q genes, specifying Qa antigens and situated in the extended part of the major histocompatibility complex (MHC) of the mouse, comprise a subgroup of MHC class I genes whose significance and function are still largely unknown. In screening a cDNA library made from the BALB/c inducer T-cell line Cl.Ly1-T1, we isolated 11 clones representing Q8/9, but none representing Q6 or Q7. Confirmatory evidence is given that the Q8/9 gene originated from fusion of the 5' region of the Q8 gene with the 3' region of the Q9 gene at a recombination site or hot spot in the vicinity of intron 4. Contrary to previous impressions that Q8/9 is an inert pseudogene, we find that the Q8/9 gene can be functional and encode a Qa-2, 3 antigen. One variety of the 11 Q8/9 clones isolated lacked exon 5, which encodes the transmembrane domain of class I glycoproteins, and thus may account for secretion of a soluble form of Qa-2, 3 antigen thought to be released by activated T cells.

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Transcripts of Tla genes

Cited by 10 publications

References 12 publications

Transcription analysis, physical mapping, and molecular characterization of a nonclassical human leukocyte antigen class I gene.

Transcription analysis, physical mapping, and molecular characterization of a nonclassical human leukocyte antigen class I gene.

Expression of CD1 in the mouse thymus

Expression of theQ8/9 d gene by T cells of the mouse

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