Transcriptomics-based identification and characterization of 11 CYP450 genes of &amp;lt;italic&amp;gt;Panax ginseng&amp;lt;/italic&amp;gt; responsive to MeJA

Xu, Zhuo; Luo, Tiao; Li, Jijia; Gui, Li; Zhou, Ding; Liu, Tuo; Zou, Xian; Pandey, Aparna; Luo, Zhitian

doi:10.1093/abbs/gmy120

Cited by 16 publications

(6 citation statements)

References 77 publications

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“…In addition, the contents of protopanaxadiol and protopanaxatriol in stems and leaves were signi cantly higher than those in roots. It is speculated that after dammarenediol-is produced in the stems and leaves, CYP12H and CYP6H continuously hydroxylate dammarenediol-to synthesize protopanaxatriol (Zeng et al 2018). Then, part of the protopanaxatriol may be transported from leaves and stems to roots, and used to synthesize ginsenoside F1.…”

Section: Discussionmentioning

confidence: 99%

Constructing a Biosynthetic Pathway of Ginsenoside F1 to Achieve Its Synthesis in Tobacco

Chen,

Lei,

et al. 2023

Preprint

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Ginsenoside F1 has high medicinal values, which is a kind of rare triterpene saponin isolated from Panax plants. The extremely low content of ginsenoside F1 in herbs has limited its research and application in medical field. In this work, we constructed a pathway in tobacco for the biosynthesis of ginsenoside F1 by metabolic engineering. Four enzyme genes (PnDDS, CYP12H, CYP6H and UGT20) isolated from Panax notoginseng were introduced into tobacco. Thus, a biosynthetic pathway for ginsenoside F1 synthesis was artificially constructed in tobacco cells; moreover, the four exogenous genes could be expressed in the roots, stems and leaves of transgenic plants. Consequently, ginsenoside F1 and its precursors were successfully synthesized in the transgenic tobacco, compared with Panax plants, the content of ginsenoside F1 in transgenic tobacco was doubled. Based on these results, a new approach was established to produce rare ginsenoside F1; meanwhile, such strategy could also be employed in plant hosts for the heterologous synthesis of other important or rare natural products.

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Section: Discussionmentioning

confidence: 99%

Constructing a Biosynthetic Pathway of Ginsenoside F1 to Achieve Its Synthesis in Tobacco

Chen,

Lei,

et al. 2023

Preprint

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“…The functions of 21 (95%) of them have been validated through MeJA regulation that has widely been used for functional validation of genes involved in ginsenoside biosynthesis in ginseng [8,[13][14][15][16][17][18]. For instance, the functions of most of the key enzyme genes cloned thus far in ginsenoside biosynthesis were validated by gene regulation with MeJA, such as PgSS [21], PgSE [15], PgMVD [30], PgFPS [31], PgDDS [32], PgUGT [33], and PgCYP450 [34]. Although the function of the one remaining candidate gene for Rb1 biosynthesis, PgRb1-20-01, has not been confirmed by the MeJA-mediated gene regulation method, it is only less than 5% out of the 22 candidate genes.…”

Section: Discussionmentioning

confidence: 99%

“…Because roots are the major organ in which ginsenosides are synthesized and stored, gene regulation with MeJA in cultured adventitious roots has widely been used to functionally validate genes that are involved in ginsenoside biosynthesis in ginseng [8,[13][14][15][16][17][18]. The functions of most of the key enzyme genes cloned thus far in ginsenoside biosynthesis were validated by this method, including PgMVD [30], PgFPS [31], PgDDS [32], PgSS [21], PgSE [15], PgUGT [33], and PgCYP450 [34]. Therefore, we validated the roles of the PgRb1 candidate genes in Rb1 biosynthesis using this method.…”

Section: Functional Validation Of Pgrb1 Candidate Genes For Rb1 Biosy...mentioning

confidence: 99%

Transcriptome and Phenotype Integrated Analysis Identifies Genes Controlling Ginsenoside Rb1 Biosynthesis and Reveals Their Interactions in the Process in Panax ginseng

Jiang

Liu

et al. 2022

IJMS

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Genes are the keys to deciphering the molecular mechanism underlying a biological trait and designing approaches desirable for plant genetic improvement. Ginseng is an important medicinal herb in which ginsenosides have been shown to be the major bioactive component; however, only a few genes involved in ginsenoside biosynthesis have been cloned through orthologue analysis. Here, we report the identification of 21 genes controlling Rb1 biosynthesis by stepwise ginseng transcriptome and Rb1 content integrated analysis. We first identified the candidate genes for Rb1 biosynthesis by integrated analysis of genes with the trait from four aspects, including gene transcript differential expression between highest- and lowest-Rb1 content cultivars, gene transcript expression–Rb1 content correlation, and biological impacts of gene mutations on Rb1 content, followed by the gene transcript co-expression network. Twenty-two candidate genes were identified, of which 21 were functionally validated for Rb1 biosynthesis by gene regulation, genetic transformation, and mutation analysis. These genes were strongly correlated in expression with the previously cloned genes encoding key enzymes for Rb1 biosynthesis. Based on the correlations, a pathway for Rb1 biosynthesis was deduced to indicate the roles of the genes in Rb1 biosynthesis. Moreover, the genes formed a strong co-expression network with the previously cloned Rb1 biosynthesis genes, and the variation in the network was associated with the variation in the Rb1 content. These results indicate that Rb1 biosynthesis is a process of correlative interactions among Rb1 biosynthesis genes. Therefore, this study provides new knowledge, 21 new genes, and 96 biomarkers for Rb1 biosynthesis useful for enhanced research and breeding in ginseng.

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“…It was reported that MeJA could not only regulate triterpenoid skeleton synthesis genes, but also those terpene modi cation genes, such as, cytochrome P450 enzymes and glycosyltransferases which make triterpenoid family the most diversi cation of con guration (Caretto et al, 2011;Kim et al, 2011;Zeng et al, 2018). As well as a key point of this study, the contents of ursane-type triterpenoids changed signi cantly, and triterpenes with more hydroxyl sites, like roxburic acid, kajiichigoside F1, euscaphic acid and pomonic acid accumulated in R. Roxburghii under the regulation of MeJA (Fig.…”

Section: Discussionmentioning

confidence: 99%

Methyl Jasmonate Promotes the Growth of Rosa Roxburghii via Balanceing Pathways referring to Indoleacetic acid (IAA) and Triterpenes.

Ning

Ding

Chang

et al. 2022

Preprint

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Exogenous phytohormones has become a common strategy to study plant stress and defense, which triggering complex and diverse cross-actions between endogenous phytohormones, which significantly regulate plant growth and defense. In this study, Methyl Jasmonate (MeJA) promoted the most obvious growth phenotype among four different exogenous phytohormones, especially promoted the growth of roots obviously. Subsequently, principal component analysis between physicochemical indexes and MeJA treatment suggested that MeJA promoted the growth of R. Roxburghii. MeJA could increase the contents of JA-Ile, IAA and triterpenoids. qPCR results showed that key genes in the JA signal transduction pathway up-regulated trends. And tryptophan synthesis genes, ANTHRANILATE AYNTHASE α1/β1 (ASA1/ASB1), IAA receptor protein gene TIR1, as well as the IAA polar transport protein gene PIN2 were all up-regulated by MeJA treatment. While, seven key genes of triterpene synthesis pathway performed a similar up-regulated trends. The results of the chord diagram analysis indicated there might be possible mutual regulation relationship among exogenous MeJA, endogenous JA-Ile, IAA and triterpenoids. The correlation analysis among triterpenoids, IAA and JA-Ile suggested that exogenous MeJA could promote the growth of Rosa Roxburghii through regulating the JA signal tranduction pathway, synthesis and transport of IAA. At the meanwhile, MeJA promoted the synthesis of triterpenoids which could not only regulate the root growth, but also make Rosa Roxburghii a certain defense ability. Finally, we speculate that exogenous MeJA pushed endogenous JA-Ile and IAA involved in regulated the metabolism of triterpenoids, they together regulated the positive growth of plants.

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Transcriptomics-based identification and characterization of 11 CYP450 genes of <italic>Panax ginseng</italic> responsive to MeJA

Cited by 16 publications

References 77 publications

Constructing a Biosynthetic Pathway of Ginsenoside F1 to Achieve Its Synthesis in Tobacco

Constructing a Biosynthetic Pathway of Ginsenoside F1 to Achieve Its Synthesis in Tobacco

Transcriptome and Phenotype Integrated Analysis Identifies Genes Controlling Ginsenoside Rb1 Biosynthesis and Reveals Their Interactions in the Process in Panax ginseng

Methyl Jasmonate Promotes the Growth of Rosa Roxburghii via Balanceing Pathways referring to Indoleacetic acid (IAA) and Triterpenes.

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