Transcriptomics and Transcriptional Regulation

Investigation of (S)-(−)-Acidomycin: A Selective Antimycobacterial Natural Product That Inhibits Biotin Synthase

“…This method was adapted from a previously-described protocol. 91 Briefly, mid-exponential growth bacteria were suspended to a final OD 580 of 0.1-0.2 in 30 mL fresh 7H9 [4.7 g/L Middlebrook 7H9 Broth Base, 2.5 g/L Bovine Serum Albumin Fraction V protease-free (Roche), 1 g/L dextrose, 0.425 g/L NaCl, 0.2% (v/v) glycerol, and 0.05% (v/v) Tyloxapol] in a vented T175 flask, and were incubated for 7 d at 37 °C and 5% CO 2 in a humid incubator. Bacteria in 20 mL of this culture were pelleted by centrifugation.…”

“…The cloning was performed as described previously. 91 The resulting plasmid pGMCZ-phsp60-SD1-BioBtb was integrated into the attachment site of the phage L5 by transformation of M. tuberculosis H37Rv and selection with zeocin.…”

Functional Genomics–Linking Genotype with Phenotype on Genome-wide Scale

“…When the human genome was fully sequenced, the focus of attention shifted towards identifying and annotating its functional DNA elements, including those that regulate gene expression (Schnappinger, 2008). Identification of such elements is a vitally important step towards elucidating pathogenic pathways that affect human health (Alderton, 2010).…”