Transcriptomics and metabolomics together reveal the underlying mechanism of heroin hepatotoxicity

Yue, Yingbiao; Zou, Lei; Tao, Jie; Li, Yin; Xie, Zhenrong; Lei, Yu; Zhang, Zunyue; Wang, Kunhua; Zhu, Mei

doi:10.1016/j.tox.2022.153393

Cited by 3 publications

(2 citation statements)

References 42 publications

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“…The samples were fully ground and centrifuged at low temperature, redissolved using acetonitrile solution, filtered and subjected to LC–MS. Detailed procedures have been reported previously 24 …”

Section: Methodsmentioning

confidence: 99%

Lung proteomics combined with metabolomics reveals molecular characteristics of inflammation‐related lung tumorigenesis induced by B(a)P and LPS

Miao

et al. 2023

Environmental Toxicology

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Inflammatory microenvironment may take a promoting role in lung tumorigenesis. However, the molecular characteristics underlying inflammation‐related lung cancer remains unknown. In this work, the inflammation‐related lung tumorigenesis mouse model was established by treated with B(a)P (1 mg/mouse, once a week for 4 weeks), followed by LPS (2.5 μg/mouse, once every 3 weeks for five times), the mice were sacrificed 30 weeks after exposure. TMT‐labeled quantitative proteomics and untargeted metabolomics were used to interrogate differentially expressed proteins and metabolites in different mouse cancer tissues, followed by integrated crosstalk between proteomics and metabolomics through Spearman's correlation analysis. The result showed that compared with the control group, 103 proteins and 37 metabolites in B(a)P/LPS group were identified as significantly altered. By searching KEGG pathway database, proteomics pathways such as Leishmaniasis, Asthma and Intestinal immune network for IgA production, metabolomics pathways such as Vascular smooth muscle contraction, Linoleic acid metabolism and cGMP‐PKG signaling pathway were enriched. A total of 22 pathways were enriched after conjoint analysis of the proteomic and metabolomics, and purine metabolism pathway, the unique metabolism‐related pathway, which included significantly altered protein (adenylate cyclase 4, ADCY4) and metabolites (L‐Glutamine, guanosine monophosphate (GMP), adenosine and guanosine) was found. Results suggested purine metabolism may contribute to the inflammation‐related lung tumorigenesis, which may provide novel clues for the therapeutic strategies of inflammation‐related lung cancer.

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Section: Methodsmentioning

confidence: 99%

Lung proteomics combined with metabolomics reveals molecular characteristics of inflammation‐related lung tumorigenesis induced by B(a)P and LPS

Miao

et al. 2023

Environmental Toxicology

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“…The samples were processed and subjected to liquid chromatography (LC)-mass spectrum (MS) detection. The assay methods and data analysis are referenced to Yue et al [27]. Briefly, the breast samples were first used for metabolite extraction, and then LC-MS determination was performed.…”

Section: Non-targeted Metabolomics Analysismentioning

confidence: 99%

Biogenic Selenium Nanoparticles Synthesized Using Alginate Oligosaccharides Attenuate Heat Stress-Induced Impairment of Breast Meat Quality via Regulating Oxidative Stress, Metabolome and Ferroptosis in Broilers

Yang,

An,

Zhang

et al. 2023

Antioxidants

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Selenium (Se) is an indispensable trace element with versatile functions in antioxidant defense in poultry. In our previous study, we synthesized a novel type of biogenic selenium nanoparticle based on alginate oligosaccharides (SeNPs-AOS), and found that the particles are sized around 80 nm with an 8% Se content, and the dietary addition of 5 mg/kg of SeNPs-AOS could effectively alleviate the deleterious effects of heat stress (HS) in broilers, but it is still unclear whether SeNPs-AOS can improve the meat quality. Therefore, the aim of this study was to evaluate the protective effects of SeNPs-AOS on breast meat quality in heat-stressed broilers, and explore the relevant mechanisms. Birds at the age of 21 days were randomly divided into four groups with six replicates per group (eight broilers per replicate) according to a 2 × 2 experimental design, using HS (33 ± 2 °C, 10 h/day vs. thermoneutral, TN, under 23 ± 1.5 °C) and SeNPs-AOS (5 mg/kg feed vs. no inclusion) as variables. The results showed that dietary SeNPs-AOS decreased the cooking loss (p < 0.05), freezing loss (p < 0.001), and shear force (p < 0.01) of breast muscle in heat-stressed broilers. The non-targeted metabolomics analysis of the breast muscle identified 78 differential metabolites between the HS and HS + SeNPs-AOS groups, mainly enriched in the arginine and proline metabolism, β-alanine metabolism, D-arginine and D-ornithine metabolism, pantothenate, and CoA biosynthesis pathways (p < 0.05). Meanwhile, supplementation with SeNPs-AOS increased the levels of the total antioxidant capacity (T-AOC), the activities of catalase (CAT) and glutathione peroxidase (GSH-Px), and decreased the content of malondialdehyde (MDA) in the breast muscle (p < 0.05) in broilers under HS exposure. Additionally, SeNPs-AOS upregulated the mRNA expression of CAT, GPX1, GPX3, heme oxygenase-1 (HO-1), masculoaponeurotic fibrosarcoma G (MafG), MafK, selenoprotein W (SELENOW), SELENOK, ferritin heavy polypeptide-1 (FTH1), Ferroportin 1 (Fpn1), and nuclear factor erythroid 2-related factor 2 (Nrf2) (p < 0.05), while it downregulated Kelch-like ECH-associated pro-36 tein 1 (Keap1) and prostaglandin-endoperoxide Synthase 2 (PTGS2) expression (p < 0.05) in broilers under HS. These findings demonstrated that the dietary addition of SeNPs-AOS mitigated HS-induced oxidative damage and metabolite changes in the breast muscle of broilers, which may be related to the regulation of the Nrf2 signaling pathway and selenoprotein synthesis. In addition, SeNPs-AOS upregulated the breast muscle gene expression of anti-ferroptosis-related molecules in broilers under HS, suggesting that SeNPs-AOS can be used as novel Se supplements against HS in broilers.

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Individual and mixture effects of BTEX occupational exposure with hematologic and hepatic profiles in petrochemical workers and the metabolic mechanism

He,

Zhong,

et al. 2024

Journal of Environmental Sciences

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Transcriptomics and metabolomics together reveal the underlying mechanism of heroin hepatotoxicity

Cited by 3 publications

References 42 publications

Lung proteomics combined with metabolomics reveals molecular characteristics of inflammation‐related lung tumorigenesis induced by B(a)P and LPS

Lung proteomics combined with metabolomics reveals molecular characteristics of inflammation‐related lung tumorigenesis induced by B(a)P and LPS

Biogenic Selenium Nanoparticles Synthesized Using Alginate Oligosaccharides Attenuate Heat Stress-Induced Impairment of Breast Meat Quality via Regulating Oxidative Stress, Metabolome and Ferroptosis in Broilers

Individual and mixture effects of BTEX occupational exposure with hematologic and hepatic profiles in petrochemical workers and the metabolic mechanism

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