Transcriptomics and Metabolomics Reveal Purine and Phenylpropanoid Metabolism Response to Drought Stress in Dendrobium sinense, an Endemic Orchid Species in Hainan Island

Zhang, Cuili; Chen, Jinhui; Huang, Weixia; Song, Xiqiang; Niu, Jun

doi:10.3389/fgene.2021.692702

Cited by 27 publications

(29 citation statements)

References 61 publications

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“…Response of plants to water-deficient conditions can be observed at transcriptomic and metabolomic levels (Figure 2) [21,86,118]. You et al [21] studied drought-tolerant (DT) and drought-susceptible (DS) sesame genotypes under drought stress and found that DS plants were more disturbed by stress conditions that was confirmed at both transcriptional and metabolic levels.…”

Section: Transcriptomic and Metabolomic Changes In Plants Under Drought Stressmentioning

confidence: 96%

“…In addition, other genes encoding for chaperones, including heat shock proteins (HSP) and small heat shock proteins (sHSP), Dna J proteins, aquaporins, orthologs of ABFs (ABA-Responsive-Element binding factors), and DREB2s (Dehydration-Responsive-Element-Binding Proteins 2), glutaredoxins (GRXs), glutathione S-transferase (GST), and many more can also be involved in plant response to drought stress [127][128][129][130][131][132][133][134][135][136]. Transcriptome analysis of the many other plants or crops such as pine (Pinus massoniana) [137], tea oil camellia (Camellia oleifera) [138], maize (Zea mays L.) [139], peanut (Arachis hypogaea L. varieties) [140], endemic orchid species (Dendrobium sinense) [118] under drought stress also provided insights into the molecular mechanism such as expression of drought stress genes and certain functional genes that helps the plant to cope with drought stress. Tahmasebi et al [141] investigated the transcriptional response of two different plant species (Oryza sativa (rice, C 3 plant) and Zea mays (maize, C 4 plant)) to drought stress based on 172 arrays in total from 11 drought stress studies.…”

Section: Transcriptomic and Metabolomic Changes In Plants Under Drought Stressmentioning

confidence: 99%

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The Impact of Drought Stress on Soil Microbial Community, Enzyme Activities and Plants

2022

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Nowadays, the most significant consequence of climate change is drought stress. Drought is one of the important, alarming, and hazardous abiotic stresses responsible for the alterations in soil environment affecting soil organisms, including microorganisms and plants. It alters the activity and functional composition of soil microorganisms that are responsible for crucial ecosystem functions and services. These stress conditions decrease microbial abundance, disturb microbial structure, decline microbial activity, including enzyme production (e.g., such as oxidoreductases, hydrolases, dehydrogenase, catalase, urease, phosphatases, β-glucosidase) and nutrient cycling, leading to a decrease in soil fertility followed by lower plant productivity and loss in economy. Interestingly, the negative effects of drought on soil can be minimized by adding organic substances such as compost, sewage slugs, or municipal solid waste that increases the activity of soil enzymes. Drought directly affects plant morphology, anatomy, physiology, and biochemistry. Its effect on plants can also be observed by changes at the transcriptomic and metabolomic levels. However, in plants, it can be mitigated by rhizosphere microbial communities, especially by plant growth-promoting bacteria (PGPB) and fungi (PGPF) that adapt their structural and functional compositions to water scarcity. This review was undertaken to discuss the impacts of drought stress on soil microbial community abundance, structure and activity, and plant growth and development, including the role of soil microorganisms in this process. Microbial activity in the soil environment was considered in terms of soil enzyme activities, pools, fluxes, and processes of terrestrial carbon (C) and nitrogen (N) cycles. A deep understanding of many aspects is necessary to explore the impacts of these extreme climate change events. We also focus on addressing the possible ways such as genome editing, molecular analysis (metagenomics, transcriptomics, and metabolomics) towards finding better solutions for mitigating drought effects and managing agricultural practices under harsh condition in a profitable manner.

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Section: Transcriptomic and Metabolomic Changes In Plants Under Drought Stressmentioning

confidence: 96%

Section: Transcriptomic and Metabolomic Changes In Plants Under Drought Stressmentioning

confidence: 99%

The Impact of Drought Stress on Soil Microbial Community, Enzyme Activities and Plants

2022

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“…With the advent of high-throughput sequencing technologies, it has become increasingly feasible to explore a single gene or gene family [ 1 , 13 , 22 ]. To identify the key type III PKS genes involved in bibenzyl biosynthesis, the roots, pseudobulbs, and leaves of D. sinense were individually sequenced by Illumina platform.…”

Section: Discussionmentioning

confidence: 99%

“…The gene functions were annotated based on the public databases, as described in our previous study [ 1 ]. Bowtie software was used to compare the clean reads with unigene database.…”

Section: Methodsmentioning

confidence: 99%

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Characterization of the Key Bibenzyl Synthase in Dendrobium sinense

Chen

Wang

Liang

et al. 2022

IJMS

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Dendrobium sinense, an endemic medicinal herb in Hainan Island, is rich in bibenzyls. However, the key rate-limited enzyme involved in bibenzyl biosynthesis has yet to be identified in D. sinense. In this study, to explore whether there is a significant difference between the D. sinense tissues, the total contents of bibenzyls were determined in roots, pseudobulbs, and leaves. The results indicated that roots had higher bibenzyl content than pseudobulbs and leaves. Subsequently, transcriptomic sequencings were conducted to excavate the genes encoding type III polyketide synthase (PKS). A total of six D. sinense PKS (DsPKS) genes were identified according to gene function annotation. Phylogenetic analysis classified the type III DsPKS genes into three groups. Importantly, the c93636.graph_c0 was clustered into bibenzyl synthase (BBS) group, named as D. sinense BBS (DsBBS). The expression analysis by FPKM and RT-qPCR indicated that DsBBS showed the highest expression levels in roots, displaying a positive correlation with bibenzyl contents in different tissues. Thus, the recombinant DsBBS-HisTag protein was constructed and expressed to study its catalytic activity. The molecular weight of the recombinant protein was verified to be approximately 45 kDa. Enzyme activity analysis indicated that the recombinant DsBBS-HisTag protein could use 4-coumaryol-CoA and malonyl-CoA as substrates for resveratrol production in vitro. The Vmax of the recombinant protein for the resveratrol production was 0.88 ± 0.07 pmol s−1 mg−1. These results improve our understanding with respect to the process of bibenzyl biosynthesis in D. sinense.

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Identification of lignans and quality assessment in Dendrobium officinale under different cultivation modes

Zhang

et al. 2023

Rapid Comm Mass Spectrometry

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Rationale Lignans have attracted much attention from researchers because of their wide distribution and industrial applications in plants, as well as the remarkable diversity of their biological activities. As the literature has mainly focused on the extraction and identification of monomeric compounds of lignans, most lignans in Dendrobium officinale, a traditional Chinese medicine with a long cultivation history and rich sources, have not been detected using quality control methods. The aim of this study was to identify the lignans in Dactilon officinale. Methods High‐performance liquid chromatography (HPLC) coupled with diode array detection and HPLC multiple‐stage tandem mass spectrometry was used to identify the chemical constituents of D. officinale. Simultaneously, the characteristic chromatograms of D. officinale were established. Additionally, a method was established to determine the content of syringaresinol‐4,4′‐di‐O‐β‐D‐glucoside, syringaresinol‐4‐O‐β‐D‐glucoside and syringaresinol. Results Thirty‐three lignans, including 17 tetrahydrofuran lignans, two dibenzylbutane lignans, three aryl tetrahydronaphthalene lignans and 11 8‐O‐4′‐neolignans, were tentatively identified from the methanol extract of the stems of D. officinale. This is the first report of 8‐O‐4′‐neolignans from D. officinale. In addition, a total of eight characteristic peaks were marked in characteristic chromatograms, which were identified as lyoniresinol‐9′‐O‐β‐D‐glucoside, syringaresinol‐4,4′‐di‐O‐β‐D‐glucoside, 8‐hydroxy‐syringaresinol‐4‐O‐β‐D‐glucoside, 5,5′‐dimethoxy‐lariciresinol‐4‐O‐β‐D‐glucoside, syringaresinol‐4‐O‐β‐D‐glucoside, 4‐hydroxy‐3,3′,5,5′‐tetramethoxy‐8,4′‐oxyneoligna‐7′‐ene‐9,9′‐diol‐9‐O‐β‐D‐glucoside, 4‐hydroxy‐3,3′,5,5′‐tetramethoxy‐8,4′‐oxyneoligna‐7′‐ene‐9,9′‐diol‐4‐O‐β‐D‐glucoside and syringaresinol. Our results showed that no significant difference occurred in lignan composition among the 99 batches of D. officinale from different sources. However, the peak areas of the lignans of D. officinale planted under simulated wild culture were generally higher than those in greenhouses, and showed an upward trend with the increase in growth years. The average contents of syringaresinol‐4,4′‐di‐O‐β‐D‐glucoside, syringaresinol‐4‐O‐β‐D‐glucoside and syringaresinol were 10.112–179.873, 51.227–222.294 and 6.368–120.341 μg/g, respectively. Conclusions This study provided a basis for improving the quality control of D. officinale and could provide references for the identification of lignans in other Dendrobium species.

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Transcriptomics and Metabolomics Reveal Purine and Phenylpropanoid Metabolism Response to Drought Stress in Dendrobium sinense, an Endemic Orchid Species in Hainan Island

Cited by 27 publications

References 61 publications

The Impact of Drought Stress on Soil Microbial Community, Enzyme Activities and Plants

The Impact of Drought Stress on Soil Microbial Community, Enzyme Activities and Plants

Characterization of the Key Bibenzyl Synthase in Dendrobium sinense

Identification of lignans and quality assessment in Dendrobium officinale under different cultivation modes

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