Transcriptomic responses to heat stress in rainbow trout Oncorhynchus mykiss head kidney

Huang, Jinxin; Li, Yongjuan; Zhe, Liu; Kang, Yujun; Wang, Jianfu

doi:10.1016/j.fsi.2018.08.002

Cited by 89 publications

(63 citation statements)

References 60 publications

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“…The hspa5 mRNA expression of rainbow trout was significantly higher at 25 and 26 °C than that at 18 °C ( p < 0.05) [35]. Based on the transcriptomic data for rainbow trout, hspa5 was a significantly upregulated gene under heat stress [36]. Moreover, five genes ( hsp70 , hspa4a , hspa5.1 , hsc70 , and hspa8b ) were significantly upregulated under heat stress.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification of Hsp70 Genes in the Large Yellow Croaker (Larimichthys crocea) and Their Regulated Expression Under Cold and Heat Stress

Han

2018

Genes

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Heat shock proteins 70 (Hsp70) are required for key cellular processes and responses to environmental changes, however, there are an unknown number of hsp70 gene family members in the large yellow croaker (Larimichthys crocea). In the present study, 17 hsp70 genes were identified through the genome of the large yellow croaker. These genes are divided into seven evolutionarily distinct groups according to a phylogenetic tree. The orthologs of these hsp70 genes were found in humans and zebrafish. The expression patterns of the hsp70 gene family in the large yellow croaker under cold and heat stress were studied by examining transcriptome data. Six out of 17 genes were significantly unregulated or downregulated after cold or heat stress. There were two genes significantly upregulated and two genes downregulated in the liver after cold treatment, while after heat treatment, five genes were significantly upregulated, and no genes were significantly downregulated. Three expression patterns were detected: strictly heat-inducible hsp70, constitutively expressed and moderately heat-inducible hsp70, and constitutively expressed and less stress-dependent hsp70 genes. All the findings will contribute to a better understanding of the biological function of hsp70s in defending against thermal challenges.

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification of Hsp70 Genes in the Large Yellow Croaker (Larimichthys crocea) and Their Regulated Expression Under Cold and Heat Stress

Han

2018

Genes

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“…So far, transcriptome analysis is one of the mainstream methods to illustrate the molecular mechanisms of lung injury induced by physical or chemical. 31 , 32 However, there have been no reports of lung injury caused by blast or shock wave using transcriptomic sequencing at present. In the study, 895 genes were identified to be differentially expressed between blast exposure group and control goats.…”

Section: Discussionmentioning

confidence: 99%

Global gene expression profiling of blast lung injury of goats exposed to shock wave

Wang

Zhang

Gao³

et al. 2020

Chinese Journal of Traumatology

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Purpose Blast lung injury (BLI) is the most common damage resulted from explosion-derived shock wave in military, terrorism and industrial accidents. However, the molecular mechanisms underlying BLI induced by shock wave are still unclear. Methods In this study, a goat BLI model was established by a fuel air explosive power. The key genes involved in were identified. The goats of the experimental group were fixed on the edge of the explosion cloud, while the goats of the control group were 3 km far away from the explosive environment. After successful modeling for 24 h, all the goats were sacrificed and the lung tissue was harvested for histopathological observation and RNA sequencing. Gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) analysis were performed to identify the main enriched biological functions of differentially expressed genes (DEGs). Quantitative real-time polymerase chain reaction (qRT-PCR) was used to verify the consistency of gene expression. Results Of the sampled goat lungs, 895 genes were identified to be significantly differentially expressed, and they were involved in 52 significantly enriched GO categories. KEGG analysis revealed that DEGs were highly enriched in 26 pathways, such as cytokine-cytokine receptor interaction, antifolate resistance, arachidonic acid metabolism, amoebiasis and bile secretion, JAK-STAT, and IL-17 signaling pathway. Furthermore, 15 key DEGs involved in the biological processes of BLI were confirmed by qRT-PCR, and the results were consistent with RNA sequencing. Conclusion Gene expression profiling provide a better understanding of the molecular mechanisms of BLI, which will help to set strategy for treating lung injury and preventing secondary lung injury induced by shock wave.

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“…The studies focused on the regulation stimulated by high or low temperature. The normal expression profiles of kidney and gill were changed after challenged with high temperature in rainbow trout and Yesso scallop, and immune-related genes were up-and down-regulated (Huang et al, 2018;Jiang et al, 2018). A series of evidences indicated the immune response stimulated by stress mediated by cortisol and glucocorticoids.…”

Section: Introductionmentioning

confidence: 99%

Impact of Ringer’s Solution Challenge Stress to Immunostimulatory Experiment, Insights From Japanese Flounder

Liu

Wang

et al. 2020

Front. Physiol.

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Ringer’s or phosphate buffer saline (PBS) solution buffer usually was used as dilution butter in intraperitoneal injection. Stress could activate immune response, inflammatory response and glycogen metabolic process. The impact of solution buffer as a stressor to immune system was ignored in immunostimulatory experiment. In this report, we tested the hypothesis that the innate immune response and glycogen metabolic process were altered when it were challenged with Ringer’s in Japanese flounder (Paralichthys olivaceus). RNA-seq was performed after challenge with Ringer’s at 8 h and 48 h. The data revealed that the expression profiles of blood, gill, and kidney were significantly changed. Differentially expressed genes (DEGs) were identified, and energy metabolic and immune-related genes were up-regulated or down-regulated obviously. GO and KEGG analyses showed that DEGs were mainly enriched in innate immune terms and pathways. Weighted gene co-expression networks analysis (WGCNA) also indicated the highest association module with stress. A total of 16 genes were detected in the gray module, which were immune-related and metabolic-related genes. These results provided fundamental information on intraperitoneal injection with solution buffer. It offered useful clues to further explore the functional mechanism of stress and immunity.

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Transcriptomic responses to heat stress in rainbow trout Oncorhynchus mykiss head kidney

Cited by 89 publications

References 60 publications

Genome-Wide Identification of Hsp70 Genes in the Large Yellow Croaker (Larimichthys crocea) and Their Regulated Expression Under Cold and Heat Stress

Genome-Wide Identification of Hsp70 Genes in the Large Yellow Croaker (Larimichthys crocea) and Their Regulated Expression Under Cold and Heat Stress

Global gene expression profiling of blast lung injury of goats exposed to shock wave

Impact of Ringer’s Solution Challenge Stress to Immunostimulatory Experiment, Insights From Japanese Flounder

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