Transcriptomic response in foliar and root tissues of a drought-tolerant Eucalyptus globulus genotype under drought stress

Ulloa, José Luis; Aguayo, Paula; Conejera, Daniel; Rubilar, Rafael; Balocchi, Claudio; Valenzuela, Sofía

doi:10.1007/s00468-021-02241-5

Cited by 4 publications

(2 citation statements)

References 79 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Upregulated genes have been reported to enhance antioxidant capacity, regulatory factors, and repressors of premature senescence under drought stress [ 19 ]. Genes involved in the protection system against reactive oxygen species, chaperones, transcription factors, and secondary metabolism are upregulated by drought stress [ 20 ]. Drought stress decreases morphological indices and increases pulegone and menthofuran content in Mentha piperita [ 21 ] and increases the biosynthesis and accumulation of bioactive compounds, such as sucrose, starch, and carotenoids, in Polygonatum kingianum tubers [ 17 ], phenolics and lignins in potato [ 22 ], and L-ascorbic acid in Pugionium cornutum [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic and physiological analysis of atractylodes chinensis in response to drought stress reveals the putative genes related to sesquiterpenoid biosynthesis

Ma,

Sun,

et al. 2024

BMC Plant Biol

View full text Add to dashboard Cite

Background Atractylodes chinensis (DC) Koidz., a dicotyledonous and hypogeal germination species, is an important medicinal plant because its rhizome is enriched in sesquiterpenes. The development and production of A. chinensis are negatively affected by drought stress, especially at the seedling stage. Understanding the molecular mechanism of A. chinensis drought stress response plays an important role in ensuring medicinal plant production and quality. In this study, A. chinensis seedlings were subjected to drought stress treatment for 0 (control), 3 (D3), and 9 days (D9). For the control, the sample was watered every two days and collected on the second morning after watering. The integration of physiological and transcriptomic analyses was carried out to investigate the effects of drought stress on A. chinensis seedlings and to reveal the molecular mechanism of its drought stress response. Results The malondialdehyde, proline, soluble sugar, and crude protein contents and antioxidative enzyme (superoxide dismutase, peroxidase, and catalase) activity were significantly increased under drought stress compared with the control. Transcriptomic analysis indicated a total of 215,665 unigenes with an average length of 759.09 bp and an N50 of 1140 bp. A total of 29,449 differentially expressed genes (DEGs) were detected between the control and D3, and 14,538 DEGs were detected between the control and D9. Under drought stress, terpenoid backbone biosynthesis had the highest number of unigenes in the metabolism of terpenoids and polyketides. To identify candidate genes involved in the sesquiterpenoid and triterpenoid biosynthetic pathways, we observed 22 unigene-encoding enzymes in the terpenoid backbone biosynthetic pathway and 15 unigene-encoding enzymes in the sesquiterpenoid and triterpenoid biosynthetic pathways under drought stress. Conclusion Our study provides transcriptome profiles and candidate genes involved in sesquiterpenoid and triterpenoid biosynthesis in A. chinensis in response to drought stress. Our results improve our understanding of how drought stress might affect sesquiterpenoid and triterpenoid biosynthetic pathways in A. chinensis.

show abstract

Section: Introductionmentioning

confidence: 99%

Transcriptomic and physiological analysis of atractylodes chinensis in response to drought stress reveals the putative genes related to sesquiterpenoid biosynthesis

Ma,

Sun,

et al. 2024

BMC Plant Biol

View full text Add to dashboard Cite

show abstract

“…(Thumma et al, 2012), Eucalyptus globulus Labill. (Spokevicius et al, 2017;Ulloa et al, 2022), and Eucalyptus cladocalyx F. Muell. (Spokevicius et al, 2017), as well as hybrids of Eucalyptus alba Reinw.…”

Section: Introductionmentioning

confidence: 99%

Transcriptional reprogramming during recovery from drought stress in Eucalyptus grandis

et al. 2023

View full text Add to dashboard Cite

The importance of drought as a constraint to agriculture and forestry is increasing with climate change. Genetic improvement of plants’ resilience is one of the mitigation strategies to curb this threat. Although recovery from drought stress is important to long term drought adaptation and has been considered as an indicator of dehydration tolerance in annual crops, this has not been well-explored in forest trees. Thus, we aimed to investigate the physiological and transcriptional changes during drought stress and rewatering in Eucalyptus grandis. We set up a greenhouse experiment where we imposed drought stress on two-year-old seedlings and rewatered the recovery group after 17 days of drought. Our measurement of leaf stomatal conductance (gs) showed that, while gs was reduced by drought stress, it fully recovered after 5 days of rewatering. RNA-seq analysis from stem samples revealed that genes related to known stress responses such as phytohormone and reactive oxygen species signaling were upregulated while genes involved in metabolism and growth were downregulated due to drought stress. We observed reprogramming of signal transduction pathways and metabolic processes at 1 day of rewatering, indicating a quick response to rewatering. Our results suggest that recovery from drought stress may entail alterations in the jasmonic acid, salicylic acid, ethylene, and brassinosteroid signaling pathways. Using co-expression network analysis, we identified hub genes including the putative orthologs of ABI1, ABF2, ABF3, HAI2, BAM1, GolS2, and SIP1 during drought and CAT2, G6PD1, ADG1, and FD-1 during recovery. Taken together, by highlighting the molecular processes and identifying key genes, this study gives an overview of the mechanisms underlying the response of E. grandis to drought stress and recovery that trees may face repeatedly throughout their long-life cycle. This provides a useful reference to the identification and further investigation of signaling pathways and target genes for future tree improvement.

show abstract

Systems Biology as a Tool to Uncover Interdisciplinary Links within the Complex Forest Tree System

Dominguez,

Abreu,

Moritz

et al. 2023

Monitoring Forest Damage With Metabolomics Methods

View full text Add to dashboard Cite

Transcriptomic response in foliar and root tissues of a drought-tolerant Eucalyptus globulus genotype under drought stress

Cited by 4 publications

References 79 publications

Transcriptomic and physiological analysis of atractylodes chinensis in response to drought stress reveals the putative genes related to sesquiterpenoid biosynthesis

Transcriptomic and physiological analysis of atractylodes chinensis in response to drought stress reveals the putative genes related to sesquiterpenoid biosynthesis

Transcriptional reprogramming during recovery from drought stress in Eucalyptus grandis

Systems Biology as a Tool to Uncover Interdisciplinary Links within the Complex Forest Tree System

Contact Info

Product

Resources

About