Transcriptomic profiling reveals the protection mechanism of bivalent inactivated bacteria vaccine Aeromonas salmonicida and Vibrio scophthalmi in turbot (Scophthalmus maximus)

Xiu, Yunji; Su, Lin; Guo, Baoshan; Yi, Jingyuan; Yang, Zongrui; Zhou, Shun

doi:10.1016/j.aquaculture.2022.738479

Cited by 3 publications

(3 citation statements)

References 32 publications

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“…Interestingly, the activation of phagosome and lysosome pathways was significantly enriched in the grass carp, C. idella , following vaccination with the oral DNA vaccine of Vibrio mimicus (Li, Zhao, et al., 2020). Similarly, turbot, S. maximus vaccinated with bivalent inactivated bacterial vaccine ( Aeromonas salmonicida and Vibrio scophthalmi ) also reported the activation of phagosome and lysosome pathways as confirmed by the head kidney transcriptome (Xiu et al., 2022). Overall, the phagosome and lysozyme pathways were activated in response to vaccination using the FBBV pellet, which indicates that the vaccination has allowed early immunological response in red hybrid tilapia.…”

Section: Discussionmentioning

confidence: 89%

Expression of immuno‐transcriptome response in red hybrid tilapia (Oreochromis sp.) hindgut following vaccination with feed‐based bivalent vaccine

Ali,

Ngalimat,

Saad

et al. 2024

Journal of Fish Diseases

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Streptococcosis and aeromoniasis are the main obstacles to sustainable tilapia production. Vaccination offered an effective method to control microbial infections. Previously, a feed‐based bivalent vaccine (FBBV) containing killed whole organisms of Streptococcus agalactiae and Aeromonas hydrophila mixed with 10% palm oil was successfully developed, which provided good protection against streptococcosis and aeromoniasis in Oreochromis sp. However, the mechanisms of immunities in vaccinated fish still need clarification. Here, the hindgut transcriptome of vaccinated and control fish was determined, as the gut displays higher affinity towards antigen uptake and nutrient absorption. The efficacy of FBBV to improve fish immunity was evaluated according to the expression of immune‐related genes in the vaccinated fish hindgut throughout the 8‐week experimental period using RT‐qPCR. The vaccinated fish hindgut at week 6 was further subjected to transcriptomic analysis due to the high expression of immune‐related genes and contained killed whole organisms. Results demonstrated the expression of immune‐related genes was in correlation with the presence of killed whole organisms in the vaccinated fish hindgut. Transcriptomic analysis has allowed the prediction of robust immune‐related pathways, including innate and adaptive immunological responses in vaccinated fish hindgut than control fish. Pathways related to the regulation of lipid metabolism and modulation of the immune system were also significantly enriched (p ≤ .05). Overall, results offer a fundamental study on understanding the immunological response in Oreochromis sp. following vaccination with the FBBV pellet and support further application to prevent bacterial diseases in aquaculture.

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Section: Discussionmentioning

confidence: 89%

Expression of immuno‐transcriptome response in red hybrid tilapia (Oreochromis sp.) hindgut following vaccination with feed‐based bivalent vaccine

Ali,

Ngalimat,

Saad

et al. 2024

Journal of Fish Diseases

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“…Further studies revealed the key role of Fc receptor (FcR) in regulating phagocytosis and bactericidal activity of B lymphocytes ( 56 , 57 ). After 2 and 4 weeks of turbot ( Scophthalmus maximus ) inoculation with the bivalent inactivated bacteria vaccine Aeromonas salmonicida and Vibrio scophthalmi , Phage-associated genes such as Calreticulin (CALR), Antigen peptide transporter 1 (TAP1), and Integrin beta-3 (αVβ3) C-type mannose receptor 2 (MRC2) were upregulated in the kidney ( 33 ). Integrin beta-5-like (ITGB5), cyptoplasmic dynein 1 intermediate chain 1 (DYNCLI1), and thrombospondin-4-B-like (THBS4B) of the Phagosome pathway were also significantly enriched in this study, indicating that the early immune response underwent antigen processing.…”

Section: Discussionmentioning

confidence: 99%

“…The phagosome pathway was activated in the head kidney of turbot inoculated with bivalent inactivated bacteria vaccine Aeromonas salmonicida and Vibrio scophthalmi . Antigenic peptide transport protein 1 (TAP1), complement fraction 3 (C3) and mannose receptor (MR) were significantly upregulated ( 33 ). However, it is often limited to the analysis of tissues such as head kidney, gill and blood at different time points after immunization or infection.…”

Section: Introductionmentioning

confidence: 99%

Splenic protection network revealed by transcriptome analysis in inactivated vaccine-immunized flounder (Paralichthys olivaceus) against Edwardsiella tarda infection

Xing²,

Tang³

et al. 2022

Front. Immunol.

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The protective immune response produced by fish after vaccination is crucial for vaccine effectiveness. Our previous studies have shown inactivated vaccine against Edwardsiella tarda can induce immune response in flounder (Paralichthys olivaceus). To elucidate the protective immune response at the genetic level, in this study, flounder was immunized with inactivated E. tarda for 5 weeks, and then they were challenged with E. tarda. The spleen was dissected at 7th day post immunization, 1st and 7th day post challenge, respectively. Transcriptome analysis showed that average of 46 million clean reads were obtained per library, while percentage of clean reads being mapped to reference genome was more than 89% in all cases, which suggested good quality of samples. As for differentially expressed genes (DEGs) identification in inactivated E. tarda groups, at 7th day post immunization, 1422 DEGs were identified and significantly enriched in innate immune-related pathways, such as Phagosome, Cell adhesion molecules and NF-kappa B signaling pathway; At 1st post challenge day, 1210 DEGs were identified and enriched to Antigen processing and presentation and Cell adhesion molecules, indicating that the pathogen was rapidly recognized and delivered; At 7th post challenge day, 1929 DEGs were identified, belonged to Toll-like receptor signaling pathway, Antigen processing and presentation, Th1 and Th2 cell differentiation and Th17 cell differentiation. Compared to 7th post immunization day, 73 immune-associated DEGs were identified at 1st post challenge day. Protein-protein interaction networks analysis revealed 11 hub genes (TLR7, TLR3, CXCR4, IFIH1, TLR8 etc), associated with recognition of pathogens and activation of innate immunity; while for 7th post challenge day, 141 immune-associated DEGs were identified. 30 hub genes (IL6, STAT1, HSP90A.1, TLR7, IL12β etc) were associated with stimulation of lymphocyte differentiation and activation of cellular immunity. Ten immune-related genes were randomly selected for RT-qPCR validation at each time point. In conclusion, data revealed protection of flounder against E. tarda infection by inactivated vaccine is mediated via immediate recognition of pathogen and subsequently activation of cellular immunity. Results give new aspect for vaccine protection cascades, is good references for vaccine evaluation.

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Transcriptome analysis reveals immune-related genes in tissues of Vibrio anguillarum-infected turbot Scophthalmus maximus

Song,

Soomro,

Dong

et al. 2023

J. Ocean. Limnol.

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Transcriptomic profiling reveals the protection mechanism of bivalent inactivated bacteria vaccine Aeromonas salmonicida and Vibrio scophthalmi in turbot (Scophthalmus maximus)

Cited by 3 publications

References 32 publications

Expression of immuno‐transcriptome response in red hybrid tilapia (Oreochromis sp.) hindgut following vaccination with feed‐based bivalent vaccine

Expression of immuno‐transcriptome response in red hybrid tilapia (Oreochromis sp.) hindgut following vaccination with feed‐based bivalent vaccine

Splenic protection network revealed by transcriptome analysis in inactivated vaccine-immunized flounder (Paralichthys olivaceus) against Edwardsiella tarda infection

Transcriptome analysis reveals immune-related genes in tissues of Vibrio anguillarum-infected turbot Scophthalmus maximus

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