Transcriptomic profiling of Bursaphelenchus xylophilus reveals differentially expressed genes in response to ethanol

Wang, Min; Wang, Linsong; Fang, Jun-Na; Du, Guicai; Zhang, Tingting; Li, Ronggui

doi:10.1016/j.molbiopara.2022.111460

Cited by 2 publications

(2 citation statements)

References 71 publications

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“…The number of PWN increased dramatically two weeks after infection [66]. Studies also showed that low concentrations of ethanol could promote the oviposition of B. xylophilus, whereas higher concentrations of ethanol could reduce the oviposition of B. xylophilus [67,68]. A low concentration of dihydroconiferylalcohol was also reported to cause serious pine wilt by promoting the reproduction of B. xylophilus [69].…”

Section: Discussionmentioning

confidence: 97%

Cinnamyl Alcohol Dehydrogenase Gene Regulates Bursaphelenchus xylophilus Reproduction and Development

Dong,

Guo,

Deng

et al. 2023

Forests

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Pine wilt disease (PWD) caused by the pine wood nematode (PWN), Bursaphelenchus xylophilus, is a globally distributed destructive disease of pine forest. To study the PWD pathogenic mechanism, the cinnamyl alcohol dehydrogenase gene (BxCAD-1) from B. xylophilus was selected. The BxCAD-1 gene was amplified by PCR from the cDNA library of B. xylophilus and cloned into the expression vector pET-15b to construct the recombinant vector pET-15b-BxCAD-1. The recombinant cinnamyl alcohol dehydrogenase protein was purified by Ni-NTA affinity chromatography from Escherichia coli BL21 (DE3) harboring pET-15b-BxCAD-1 induced by IPTG. The effects of pH, temperature, metal ions and substrates on the activity of BxCAD-1 were determined, showing the highest catalytic activity at pH 8.0 and 40 °C with cinnamyl alcohol as substrate and Zn2+ as an activator. To elucidate the functions of BxCAD-1 in B. xylophilus, the expression of the gene was down-regulated by RNA interference. Results showed that the movement, feeding, reproduction, spawning rate, hatching rate, lifespan, infectivity and sensitivity to ethanol decreased compared with negative controls. RNA interference also affected the development of B. xylophilus from the larval stage to the adult stage. In situ hybridization showed that the gene was expressed in the digestive tract of male and female adults. This study revealed a promising target for PWD control.

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Section: Discussionmentioning

confidence: 97%

Cinnamyl Alcohol Dehydrogenase Gene Regulates Bursaphelenchus xylophilus Reproduction and Development

Dong,

Guo,

Deng

et al. 2023

Forests

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“…Subsequently, the infected pines produce ethanol, monoterpenes and other volatile compounds ( Ikeda and Oda, 1980 ). In our recent study, we found that a low concentration of ethanol derived by the host tree could promote the growth of B. xylophilus population and that a UGT gene-encoded enzyme UDP-glycosyltransferase (UGT) was significantly upregulated when PWNs were treated with ethanol ( Wang et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

UGT440A1 Is Associated With Motility, Reproduction, and Pathogenicity of the Plant-Parasitic Nematode Bursaphelenchus xylophilus

Wang

Fang

et al. 2022

Front. Plant Sci.

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Pine wilt disease (PWD) caused by Bursaphelenchus xylophilus is considered a major threat to pine forests worldwide. Uridine diphosphate (UDP)-glycosyltransferases (UGTs) catalyze the conjugation of small lipophilic compounds with sugars and play crucial roles in the detoxification and homeostatic processes in all living organisms. We investigated the molecular characteristics and biological functions of the gene UGT440A1 that encodes UGTs in B. xylophilus. The in situ hybridization results indicated that UGT440A1 is expressed in all developmental stages of B. xylophilus, particularly in the head, intestine, and hypodermis of the second-stage of juveniles (J2), third-stage of juveniles (J3) and fourth-stage of juveniles (J4) females and in almost the whole body of J4 males and adults. Recombinant UGT440A1 was observed mainly in the inclusion bodies, and the enzyme activity assay revealed that UGT440A1 could catalyze the glycosylation reaction of two types of flavonols (kaempferol and quercetin). RNA interference (RNAi) of UGT440A1 suppressed motility, feeding, and reproduction of B. xylophilus. Furthermore, UGT440A1 knockdown caused a delay in the development of PWD symptoms in the pine seedlings inoculated with the nematodes. These results suggest that UGT440A1 is involved in the pathogenic process of B. xylophilus and the information may facilitate a better understanding of the molecular mechanism of PWD.

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Transcriptomic profiling of Bursaphelenchus xylophilus reveals differentially expressed genes in response to ethanol

Cited by 2 publications

References 71 publications

Cinnamyl Alcohol Dehydrogenase Gene Regulates Bursaphelenchus xylophilus Reproduction and Development

Cinnamyl Alcohol Dehydrogenase Gene Regulates Bursaphelenchus xylophilus Reproduction and Development

UGT440A1 Is Associated With Motility, Reproduction, and Pathogenicity of the Plant-Parasitic Nematode Bursaphelenchus xylophilus

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