Transcriptomic profiling in Silurana tropicalis testes exposed to finasteride

Bissegger, Sonja; Martyniuk, Christopher J.; Langlois, Valérie S.

doi:10.1016/j.ygcen.2014.01.018

Cited by 9 publications

(7 citation statements)

References 62 publications

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“…DHT is known to promote neurogenesis and cell survival in the hippocampus, a pivotal region related to sexual arousal and libido . Other animal studies have demonstrated that finasteride induces cellular damage in testicular tissue via oxidative damage, suggesting multiple mechanisms of this adverse drug reaction.…”

Section: Discussionmentioning

confidence: 99%

Persistent Sexual Dysfunction with Finasteride 1 mg Taken for Hair Loss

et al. 2016

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Section: Discussionmentioning

confidence: 99%

Persistent Sexual Dysfunction with Finasteride 1 mg Taken for Hair Loss

et al. 2016

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“…Previous time dependent experiments (2-10 h) performed by Bissegger et al, (2014) showed that RNA degradation was not evident with the time elapsed between dissection of tissues and treatment incubations, and an incubation times of 6 h or less. The individual eight whole-testes or ovary-pieces were then placed in eight separate designated wells in 24-well plates containing either 500 µL L-15 media (control samples) or L-15 media containing T3 (50 nM; Sigma, Oakville, Ontario, CA), IOP (10 µM; TCI America), or one co-treatment of IOP (10 µM, TCI America) + 5α-DHT (1 µM; Steraloids, Newport, RI, USA).…”

Section: Animals and Exposurementioning

confidence: 93%

“…Two ex vivo assays were performed following methods of Bissegger et al (2014). In the first ex vivo assay, we examined whether TH status affects sex steroid-related transcription and hormone production in testes and ovaries of sexually mature juvenile S. tropicalis.…”

Section: Animals and Exposurementioning

confidence: 99%

Thyroid hormones and androgens differentially regulate gene expression in testes and ovaries of sexually mature Silurana tropicalis

Campbell

Langlois

2018

General and Comparative Endocrinology

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A series of ex vivo exposures using testicular and ovarian tissues of sexually mature Western clawed frogs (Silurana tropicalis) were designed to examine molecular mechanisms of thyroid hormone (TH) and androgen crosstalk sans hypophyseal feedback as well as investigate potential sex-specific differences. Tissues were exposed ex vivo to either triiodothyronine (T3), iopanoic acid (IOP), one co-treatment of IOP + 5α-dihydrotestosterone (5α-DHT), 5α-DHT, 5β-dihydrotestosterone (5β-DHT), or testosterone (T). Direct exposure to different androgens led to androgen specific increases in thyroid receptor and deiodinase transcripts in testes (trβ and dio1) but a decrease in expression in ovaries (trβ and dio3), suggesting that male and female frogs can be differently affected by androgenic compounds. Moreover, exposure to select androgens differentially increased estrogen-related transcription (estrogen receptor alpha (erα) and aromatase (cyp19)) and production (estradiol) in ovaries and testes indicating the activation of alternate metabolic pathways yielding estrogenic metabolites. Sex-steroid-related transcription (i.e., steroid 5α-reductase type 2 (srd5α2) and erα) and production (i.e., 5α-DHT) were also differentially regulated by THs. The presence and frequency of transcription factor binding sites in the putative promoter regions of TH- and sex steroid-related genes were also examined in S. tropicalis, rodent, and fish models using in silico analysis. In summary, this study provides an improved mechanistic understanding of TH- and androgen-mediated actions and reveals differential transcriptional effects as a function of sex in frogs.

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“…A total of 1,390 islands and more than 3,850 reefs are distributed in the sea area of the Zhoushan Archipelago. Many sessile organisms inhabit these rocky reefs (Saito et al, 2008;Bissegger et al, 2014). The excellent natural conditions of these sea areas provide an ideal habitat for black sea bream.…”

Section: Distribution Of Black Sea Breammentioning

confidence: 99%

Fishery resource monitoring of the East China Sea via environmental DNA approach: a case study using black sea bream (Acanthopagrus schlegelii)

Zhang

Zhou²,

Zhang³

et al. 2022

Front. Mar. Sci.

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Understanding fish stock distribution is crucial in the context of sustainable fishery development. Traditional approaches such as net catching, mark recapturing, and visual counting are often time-consuming, less effective, and prone to subjective bias. Recently, environmental DNA (eDNA) technology has made up for the shortcomings of traditional fishery resource survey methods and has been widely used for species monitoring, biodiversity assessment, and biomass estimation. In this study, the eDNA method was used to analyze the potential distribution range of the black sea bream (Acanthopagrus schlegelii) in the East China Sea. The black sea bream-specific primers and probe were designed and applied in the current study. Results showed high black sea bream eDNA concentrations in Dasha fishing ground, Yangtze Estuary fishing ground, and Zhoushan fishing ground. Higher concentrations of black sea bream eDNA were found in water depths approximately 30–40 m. The eDNA hotspots were mainly concentrated at 29°N–33°N, 122°E–125°E, but no significant differences in horizontal or vertical distributions were observed. Temperature is a critical factor in the presence and black sea bream eDNA among environmental variables in the correlation analysis. In addition, a linear formula between environmental factors and the black sea bream eDNA concentration of black sea bream was predicted, which may be helpful for future fishery resource surveys. To summarize, the present paper provides a new idea for marine resource surveys. It also affords a method and theoretical basis for applying the eDNA technology in fishery surveys, which is expected to become an essential tool for fishery resource surveys in the future.

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Transcriptomic profiling in Silurana tropicalis testes exposed to finasteride

Cited by 9 publications

References 62 publications

Persistent Sexual Dysfunction with Finasteride 1 mg Taken for Hair Loss

Persistent Sexual Dysfunction with Finasteride 1 mg Taken for Hair Loss

Thyroid hormones and androgens differentially regulate gene expression in testes and ovaries of sexually mature Silurana tropicalis

Fishery resource monitoring of the East China Sea via environmental DNA approach: a case study using black sea bream (Acanthopagrus schlegelii)

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