Transcriptomic profiling identifies novel transcripts, isomorphs, and noncoding RNAs in Paracoccidioides brasiliensis

Abstract

This paper describes a transcriptomic profiling of Paracoccidioides brasiliensis (Pb) performed with the aid of an RNA-seq-based approach, aimed at characterizing the general transcriptome in this human pathogenic fungus, responsible for paracoccidioidomycosis (PCM). Results confirm that ∼75% of the genes currently annotated in the P. brasiliensis genome are, in fact, transcribed in vivo and that ∼19% of them may display alternative isomorphs. Moreover, we identified 627 transcripts that do not match any gene currently mapped in the genome, represented by 114 coding transcripts (probably derived from previously unmapped protein-coding genes) and 513 noncoding RNAs (ncRNAs), including 203 long-noncoding RNAs (lncRNAs).

The Identification and Comparative Analysis of Non-Coding RNAs in Spores and Mycelia of Penicillium expansum

“…They play crucial roles in transcription and translation regulation, RNA metabolism, epigenetic control and signaling through cis interactions with RNAs, and through trans interactions with DNA, RNAs, or proteins [53][54][55]. Based on the accumulation of genomic knowledge and the expanding lncRNA repository, the identification and functional verification of lncRNAs in phytopathogens were also conducted [56,57]. Donaldson and Saville (2013) identified 204 natural antisense transcripts in Ustilago maydis, of which as-um02151 could positively influence pathogenesis in both seedling and cob infections of Zea [58].…”