Transcriptomic Profiling Analyses Revealed Candidate Genes Under Freezing Stress in Siberian Apricot (Prunus sibirica)

Abstract

Siberian apricot (Prunus sibirica) is an important ecological and commercial woody plant that is negatively affected by spring frosts. However, the mechanisms that control gene expression in adaptation to freezing remain largely unknown. In this work, we investigated the physiological, molecular, and phenotypic characteristics of pistils of two P. sibirica clones that differ in their ability to withstand freezing stress. A total of 14,717 unigenes categorized into 38 functional groups were identified. Additionally, the two P. sibirica clones included 3931 up-regulated and 2070 down-regulated differentially expressed genes (DEGs). Many DEGs are related to Ca2+ and MAPK signaling, carbohydrate biosynthesis and metabolism, plant hormone signal transduction, biosynthesis of amino acids, and photosynthesis. The metabolism of carbohydrates, amino acids, lipids, secondary metabolites, plant hormone signal transduction, and terpenoid metabolism were the transcriptome modifications most significantly altered by freezing stress. Real-time quantitative PCR (RT-qPCR) was used to verify the precision of the RNA-seq data. PsbHLH18, PsMYB4, PsMYB44, PsPOD1, and PsCDPK5 may play important roles in the freezing tolerance of the P. sibirica floral organ. This study provides a foundation for further studies on the complex mechanisms of freezing stress response in P. sibirica.