Transcriptomic landscape of Dendrobium huoshanense and its genes related to polysaccharide biosynthesis

Han, Richou; Xie, Dong-Mei; Tong, Xiaohui; Zhang, Wei; Liu, Gang; Peng, Daiyin; Yu, Nianjun

doi:10.5586/asbp.3574

Cited by 8 publications

(12 citation statements)

References 29 publications

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“…Mannose is one of the important components in D. huoshanense polysaccharide and Han et al have explored six related genes in mannose biosynthetic pathway 22 . In our study, ten related genes from fructose and mannose metabolism have been explored.…”

Section: Identification Of Degs In Polysaccharide and Flavonoid Biosymentioning

confidence: 99%

“…Previous work focused on identification and analysis glycosyltransferases (GTs) in polysaccharides biosynthesis 20 . Besides GTs, some metabolic pathways such as fructose and mannose metabolism may also participated in polysaccharides biosynthesis 21,22 . However, the biosynthesis mechanisms and metabolic pathways of polysaccharides have been not explored detailly in D. huoshanense.In China, the stem of Dendrobium is a traditional edible and medicinal part 2 .…”

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confidence: 99%

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Transcriptome Analysis Reveals Biosynthesis of Important Bioactive Constituents and Mechanism of Stem Formation of Dendrobium huoshanense

Zhou

Gui

et al. 2020

Sci Rep

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the stem of Dendrobium huoshanense c.Z. tang and S.J. cheng was widely used as a medicinal herb in health care products due to its broad pharmacological activities. However, the molecular regulation mechanism of stem development and biosynthetic pathways of important bioactive substances are still unclear in D. huoshanense. in this study, the bioactive compounds in leaves, stems and roots, and the identification of candidate genes involved in stem formation and biosynthesis of active compounds via transcriptome sequence were analyzed. The accumulation of total polysaccharides and flavonoids were varied significantly in different tissues. A comparative transcriptomic analysis revealed several differentially expressed genes (DEGs) involved in polysaccharides biosynthesis (103 genes), including fructose and mannose related genes (29 genes) and glycosyltransferase genes (74 genes), and flavonoids biosynthesis (15 genes). Some candidate genes that participated in photoperiod regulation (27 genes), starch and sucrose metabolism (46 genes), and hormone-induced activation of signaling pathways (38 genes) may be involved in stem formation. In sum, this study provides a foundation for investigating the molecular processes in the biosynthesis of active compounds and stem development. the transcriptome data presented here provides an important resource for the future studies of the molecular genetics and functional genomics in D. huoshanense and optimized control of the active compounds produced by D. huoshanense.Dendrobium huoshanense C.Z. Tang and S.J. Cheng is an edible and medicinal plant, and has been utilized as a tonic in Traditional Chinese Medicine for centuries 1 . Specifically, it is used for boosting immunity, slowing aging, and possesses anti-tumor properties 2,3 . At present, increased demands and over-exploration caused reduced yield of wild resource and the damage of D. huoshanense habitats. Although artificial cultivation is an efficient way to protect natural resources and develop a sustainable medicinal supply, it was still faced an important issue to ensure and improve the quality and yield of D. huoshanense under artificial conditions. Understanding the regulation mechanism of quality and production of D. huoshanense has huge commercial and health value.At present, several ingredients from D. huoshanense have been identified as therapeutic active components, such as polysaccharides 4,5 , flavonoids 6,7 , alkaloids 8,9 . Modern pharmacological researches have confirmed that polysaccharides can activate immune cells, modulate the immune responses in the intestines, spleen, and liver, and inhibit cell proliferation in tumors [10][11][12][13] . Alkaloids possessed the functions of anti-inflammatory, anti-diabetic effect and anticancer activity 14,15 . Flavonoids also possesses biological functions including anti-oxidant, anti-cancer, anti-aging, cardiovascular protection, and building of immunity [16][17][18][19] . The key genes associated with polysaccharides and alkaloids biosynthesis have been identified,...

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Section: Identification Of Degs In Polysaccharide and Flavonoid Biosymentioning

confidence: 99%

mentioning

confidence: 99%

Transcriptome Analysis Reveals Biosynthesis of Important Bioactive Constituents and Mechanism of Stem Formation of Dendrobium huoshanense

Zhou

Gui

et al. 2020

Sci Rep

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“…Annotated as “GTP-mannose-1-phosphate guanyltransferase” from deep transcriptomic data of D. huoshanense [ 13 ], the full-length DhGMPP cDNA (1,867 bp) was amplified and sequenced. DhGMPP (accession no.…”

Section: Resultsmentioning

confidence: 99%

“…GDP-mannose pyrophosphorylase gene ( DhGMPP ) is a key factor to yield GDP-mannose and ultimately DhPs. By digging into D. huoshanense RNA-Seq data [ 13 ], we cloned a DhGMPP which was then functionally expressed in Escherichia coli . The function of DhGMPP recombinant protein to catalyze the reaction of α -D-mannose-phosphate and GTP to form GDP-D-mannose was also characterized ( Figure 1 ).…”

Section: Introductionmentioning

confidence: 99%

Characterization of GMPP from Dendrobium huoshanense yielding GDP-D-mannose

Liu²,

Zhou³

et al. 2021

Open Life Sciences

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Dendrobium huoshanense has been used for centuries in China and its polysaccharides are the main active components in treating loss of body fluids resulting from fever and asthenic symptoms. However, the biosynthetic pathway of polysaccharides in D. huoshanense remains to be elucidated. In this study, we obtained a guanosine diphosphate (GDP)-mannose pyrophosphorylase (DhGMPP) from D. huoshanense and characterized its function to catalyze the conversion of α-D-mannose-phosphate to GDP-D-mannose involved in the production of polysaccharides. DhGMPP, with the open reading frame of 1,245 bp, was isolated from RNA-Seq data of D. huoshanense. Phylogenetic analysis as well as sequence characterization suggested its involvement in the biosynthesis of GDP-D-mannose. In vitro enzyme assay demonstrated that GMPP encoded a pyrophosphorylase that converted α-D-mannose-phosphate and GTP into GDP-D-mannose. Identification of DhGMPP could provide more insights into the mechanism concerning polysaccharide biosynthesis in D. huoshanense and be utilized for enhancing polysaccharide accumulation through metabolic engineering.

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“…It also shows that wild D. huoshanense has the highest nutritional value, followed by indoors. Numerous studies have shown that the cloning and expression of key enzyme genes in plant secondary metabolic pathways can increase the yield of secondary metabolites in plants, understand their biosynthetic pathways and regulatory mechanisms and regulate key genes affecting secondary metabolites (Han et al 2018). To increase its expression, thereby promoting the production of the desired product.…”

Section: Discussionmentioning

confidence: 99%

Effects of different treatments on genes related to polysaccharide content in Dendrobium huoshanense

et al. 2020

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Polysaccharide is the main active component of Dendrobium huoshanense, and it is also an important indicator to evaluate its quality. In this study, the polysaccharides and invertase components in the stems and leaves of D. huoshanense were studied to provide a theoretical basis for the full exploitation and utilization of its resources. Taking the D. huoshanense grown in the wild, indoors and outdoors as a test material and choosing stems and roots of D. huoshanense for 1 year, 2 years and 3 years of growth, the polysaccharide content of different samples was determined, and the relative expression activity of sucrose metabolism-related genes in different samples was detected by qRT-PCR. Western blot was used to detect the changes of sucrose metabolism-related proteins in different samples. The results showed that the content of polysaccharide in the wild D. huoshanense was the highest, the indoor growth was the second, the growth in the outdoor was the lowest, and the content of polysaccharide in the stem was significantly higher than that in the root. The expression of D. huoshanense neutral/alkaline invertase (DhNI), D. huoshanense sucrose synthase (DHSS) and D. huoshanense sucrose phosphate synthase (DHSPS) was the highest in wild D. huoshanense, followed by indoor growth, and the expression in stem was much higher than in root. Western blot results also indicated that DhNI, DHSS and DHSPS protein expression was highest in wild, followed by indoors. The stem is much higher than the root and is grown for 2 years in the same growing environment for more than 1 year and 3 years. The above results show that the nutritional value of D. huoshanense is related to the decomposition of sucrose and the accumulation of polysaccharides in plants. The experimental results provide a theoretical basis for better exploitation and utilization of D. huoshanense resources.

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Transcriptomic landscape of Dendrobium huoshanense and its genes related to polysaccharide biosynthesis

Cited by 8 publications

References 29 publications

Transcriptome Analysis Reveals Biosynthesis of Important Bioactive Constituents and Mechanism of Stem Formation of Dendrobium huoshanense

Transcriptome Analysis Reveals Biosynthesis of Important Bioactive Constituents and Mechanism of Stem Formation of Dendrobium huoshanense

Characterization of GMPP from Dendrobium huoshanense yielding GDP-D-mannose

Effects of different treatments on genes related to polysaccharide content in Dendrobium huoshanense

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