Transcriptomic events associated with internal browning of apple during postharvest storage

Mellidou, Ιfigeneia; Buts, Kim; Hatoum, Darwish; Ho, Quang Tri; Johnston, Jason W.; Watkins, Christopher B.; Schaffer, Robert J.; Gapper, Nigel E.; Giovannoni, Jim; Rudell, David R.; Hertog, Maarten; Nicolaı̈, Bart

doi:10.1186/s12870-014-0328-x

Cited by 77 publications

(54 citation statements)

References 62 publications

(75 reference statements)

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“…Transcriptomic studies uncovered that prolonged postharvest storage downregulated DHAR expression resulting in the irreversibly oxidation of AsA and thus enabling browning to occur (Mellidou et al, 2014). Furthermore, DHAR expression has been correlated with AsA accumulation in chestnut rose (Huang et al, 2014), and in blueberry (Liu et al, 2015).…”

Section: Genetic Regulation Through Enhancing Recyclingmentioning

confidence: 99%

Genetic Control of Ascorbic Acid Biosynthesis and Recycling in Horticultural Crops

Mellidou

Kanellis

2017

Front. Chem.

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Ascorbic acid (AsA) is an essential compound present in almost all living organisms that has important functions in several aspects of plant growth and development, hormone signaling, as well as stress defense networks. In recent years, the genetic regulation of AsA metabolic pathways has received much attention due to its beneficial role in human diet. Despite the great variability within species, genotypes, tissues and developmental stages, AsA accumulation is considered to be controlled by the fine orchestration of net biosynthesis, recycling, degradation/oxidation, and/or intercellular and intracellular transport. To date, several structural genes from the AsA metabolic pathways and transcription factors are considered to significantly affect AsA in plant tissues, either at the level of activity, transcription or translation via feedback inhibition. Yet, all the emerging studies support the notion that the steps proceeding through GDP-L-galactose phosphorylase and to a lesser extent through GDP-D-mannose-3,5-epimerase are control points in governing AsA pool size in several species. In this mini review, we discuss the current consensus of the genetic regulation of AsA biosynthesis and recycling, with a focus on horticultural crops. The aspects of AsA degradation and transport are not discussed herein. Novel insights of how this multifaceted trait is regulated are critical to prioritize candidate genes for follow-up studies toward improving the nutritional value of fruits and vegetables.

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Section: Genetic Regulation Through Enhancing Recyclingmentioning

confidence: 99%

Genetic Control of Ascorbic Acid Biosynthesis and Recycling in Horticultural Crops

Mellidou

Kanellis

2017

Front. Chem.

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“…Little data about gene expression storage is available although a transcriptomic study of an cortex disorder of apples during CA storage revealed gene expression divergences prior to symptom development [22]. …”

Section: Introductionmentioning

confidence: 99%

Delayed response to cold stress is characterized by successive metabolic shifts culminating in apple fruit peel necrosis

et al. 2017

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BackgroundSuperficial scald is a physiological disorder of apple fruit characterized by sunken, necrotic lesions appearing after prolonged cold storage, although initial injury occurs much earlier in the storage period.To determine the degree to which the transition to cell death is an active process and specific metabolism involved, untargeted metabolic and transcriptomic profiling was used to follow metabolism of peel tissue over 180 d of cold storage.ResultsThe metabolome and transcriptome of peel destined to develop scald began to diverge from peel where scald was controlled using antioxidant (diphenylamine; DPA) or rendered insensitive to ethylene using 1-methylcyclopropene (1-MCP) beginning between 30 and 60 days of storage. Overall metabolic and transcriptomic shifts, representing multiple pathways and processes, occurred alongside α-farnesene oxidation and, later, methanol production alongside symptom development.ConclusionsResults indicate this form of peel necrosis is a product of an active metabolic transition involving multiple pathways triggered by chilling temperatures at cold storage inception rather than physical injury. Among multiple other pathways, enhanced methanol and methyl ester levels alongside upregulated pectin methylesterases are unique to peel that is developing scald symptoms similar to injury resulting from mechanical stress and herbivory in other plants.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-017-1030-6) contains supplementary material, which is available to authorized users.

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“…These projects include de novo transcriptome analysis and reference mapping of expressed transcripts [ 14 – 16 ]. Using RNA- sequencing (RNA-seq) techniques, Mellidou et al [ 17 ] identified different expressed gene (DEGs) mainly involved in lipid metabolism, secondary metabolism, and cell wall modifications in apple fruit browning disorder. The energy-related and stress-related genes were also altered during apple fruit browning development.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Transcriptome and Expression Profile Analysis of Phalaenopsis during Explant Browning

Zeng

Junmei

et al. 2015

PLoS ONE

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BackgroundExplant browning presents a major problem for in vitro culture, and can lead to the death of the explant and failure of regeneration. Considerable work has examined the physiological mechanisms underlying Phalaenopsis leaf explant browning, but the molecular mechanisms of browning remain elusive. In this study, we used whole genome RNA sequencing to examine Phalaenopsis leaf explant browning at genome-wide level.Methodology/Principal FindingsWe first used Illumina high-throughput technology to sequence the transcriptome of Phalaenopsis and then performed de novo transcriptome assembly. We assembled 79,434,350 clean reads into 31,708 isogenes and generated 26,565 annotated unigenes. We assigned Gene Ontology (GO) terms, Kyoto Encyclopedia of Genes and Genomes (KEGG) annotations, and potential Pfam domains to each transcript. Using the transcriptome data as a reference, we next analyzed the differential gene expression of explants cultured for 0, 3, and 6 d, respectively. We then identified differentially expressed genes (DEGs) before and after Phalaenopsis explant browning. We also performed GO, KEGG functional enrichment and Pfam analysis of all DEGs. Finally, we selected 11 genes for quantitative real-time PCR (qPCR) analysis to confirm the expression profile analysis.Conclusions/SignificanceHere, we report the first comprehensive analysis of transcriptome and expression profiles during Phalaenopsis explant browning. Our results suggest that Phalaenopsis explant browning may be due in part to gene expression changes that affect the secondary metabolism, such as: phenylpropanoid pathway and flavonoid biosynthesis. Genes involved in photosynthesis and ATPase activity have been found to be changed at transcription level; these changes may perturb energy metabolism and thus lead to the decay of plant cells and tissues. This study provides comprehensive gene expression data for Phalaenopsis browning. Our data constitute an important resource for further functional studies to prevent explant browning.

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Transcriptomic events associated with internal browning of apple during postharvest storage

Cited by 77 publications

References 62 publications

Genetic Control of Ascorbic Acid Biosynthesis and Recycling in Horticultural Crops

Genetic Control of Ascorbic Acid Biosynthesis and Recycling in Horticultural Crops

Delayed response to cold stress is characterized by successive metabolic shifts culminating in apple fruit peel necrosis

Genome-Wide Transcriptome and Expression Profile Analysis of Phalaenopsis during Explant Browning

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