Transcriptomic effects of the non-steroidal anti-inflammatory drug Ibuprofen in the marine bivalve Mytilus galloprovincialis Lam.

Maria, V.L.; Amorim, Mónica J.B.; Bebianno, Maria João; Dondero, Francesco

doi:10.1016/j.marenvres.2016.05.010

Cited by 18 publications

(4 citation statements)

References 68 publications

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“…Since aquatic wildlife may possess conserved targets related to the drug metabolism, pharmaceuticals are potentially able to alter essential homeostatic mechanisms even at concentrations lower than those for human dosage (Fabbri and Franzellitti, 2015;Maria et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Long-term exposure of Mytilus galloprovincialis to diclofenac, Ibuprofen and Ketoprofen: Insights into bioavailability, biomarkers and transcriptomic changes

et al. 2018

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Section: Discussionmentioning

confidence: 99%

Long-term exposure of Mytilus galloprovincialis to diclofenac, Ibuprofen and Ketoprofen: Insights into bioavailability, biomarkers and transcriptomic changes

et al. 2018

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“…after exposure of organisms to different concentrations of Gd. Due to their sedentary and filtration behavior, as well as their capacity to respond to environmental alterations, M. galloprovincialis mussels are widely used as bioindicators of a vast diversity of pollutants, including classical elements such as metals (among others, (Coppola et al, 2018;Maanan, 2007;Mejdoub et al, 2018;Regoli and Principato, 1995) and compounds considered of emerging concern including pharmaceuticals (Balbi et al, 2018;Maria et al, 2016), and nanoparticles (Andrade et al, 2018;Auguste et al, 2018;Taze et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Toxicological assessment of anthropogenic Gadolinium in seawater: Biochemical effects in mussels Mytilus galloprovincialis

Henriques

Coppola

Monteiro

et al. 2019

Science of The Total Environment

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Recently, anthropogenic enrichment of rare earth elements (REE) have been reported in natural environments, due to increasing use and discharges of hospital/industrial wastewaters. Gadolinium (Gd), which is mainly used as contrast agent for magnetic resonance imaging in medical exams, may reach concentrations in water up to two orders of magnitude larger than baseline levels. Nevertheless, in marine systems scarce information is available concerning the toxicity of REE towards inhabiting organisms. This study aimed to evaluate the biochemical impact of anthropogenic Gd in the Mediterranean mussel Mytilus galloprovincialis, which is a species of commercial interest and one of the most accepted pollution bioindicator. Organisms were exposed to different concentrations of Gd (0, 15, 30, 60, 120 µg/L) for 28 days. At the end of the experiments, biomarkers related to mussels' metabolic (electron transport system activity and energy reserves content), oxidative stress status (cellular damage and the activity of antioxidant and biotransformation enzymes) and neurotoxic effects (activity of the enzyme Acetylcholinesterase) were measured, as well as Gd bioconcentration in organisms. Results showed a high content of Gd (2.5±0.50 µg/g) in mussels exposed to the highest concentration, contrary to those at control condition and at 15 and 30 μg/L of Gd (levels below 0.38 µg/g). Although no mortality was observed during the experimental period, exposure to Gd strongly affected the biochemical performance of M. galloprovincialis, including the decrease on mussels' metabolism, induction of oxidative stress and neurotoxicity, particularly evidenced at intermediate concentrations. These results may indicate that up to certain stressful levels, although lowering their metabolism, organisms may be able to activate defense strategies to avoid cellular injuries which, on the other hand, may compromise mussels physiological performance such as growth and reproduction success. Nevertheless, our findings support that the widespread utilization of Gd may represent an environmental risk in the future.

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“…To our knowledge, there are only two M. galloprovincialis microarrays previous to this design: Mytarray V1.1 (Venier et al, 2006), a cDNA microarray used mainly to study pollution effects on mussel (Dondero et al, 2010;Maria et al, 2016); and M. galloprovincialis Inmunochip (Venier et al, 2011). This is the first microarray which contains probes from several adult tissues, including hemocytes, and also from different developmental stages.…”

Section: --mentioning

confidence: 99%

Revealing Mytilus galloprovincialis transcriptomic profiles during ontogeny

Moreira

Pereiro

Balseiro

et al. 2018

Developmental & Comparative Immunology

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Mediterranean mussels are a worldwide spread bivalve species with extraordinary biological success. One of the reasons of this success could be the reproduction strategy of bivalves, characterized by the presence of trochophore larvae. Larval development in bivalves has been a topic of raising interest in the scientific community but it deserves much more attention. The principal objective of this work was to study the transcriptomic profile of the ontogeny of Mytilus galloprovincialis analyzing the gene expression in different developmental stages, from oocytes to juveniles. For this purpose, after conducting a 454 sequencing of the transcriptomes of mussel hemocytes, adult tissues and larvae, a new DNA microarray was designed and developed. The studied developmental stages: unfertilized oocytes, veliger, pediveliger, settled larvae and juveniles, showed very different transcriptomic profiles and clustered in groups defining their characteristic gene expression along ontogeny. Our results show that oocytes present a distinct and characteristic transcriptome. After metamorphosis, both settled larvae and juveniles showed a very similar transcriptome, with no enriched GO terms found between these two stages. This suggests: 1.- the progressive loss of RNA of maternal origin through larval development and 2.- the stabilization of the gene expression after settlement. On the other hand during metamorphosis a specific profile of differentially expressed genes was found. These genes were related to processes such as differentiation and biosynthesis. Processes related to the immune response were strongly down regulated. These suggest a development commitment at the expense of other non-essential functions, which are temporary set aside. Immune genes such as antimicrobial peptides suffer a decreased expression during metamorphosis. In fact, we found that the oocytes which express a higher quantity of genes such as myticins are more likely to reach success of the offspring, compared to oocytes poor in such mRNAs, whose progeny died before reaching metamorphosis.

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Transcriptomic effects of the non-steroidal anti-inflammatory drug Ibuprofen in the marine bivalve Mytilus galloprovincialis Lam.

Cited by 18 publications

References 68 publications

Long-term exposure of Mytilus galloprovincialis to diclofenac, Ibuprofen and Ketoprofen: Insights into bioavailability, biomarkers and transcriptomic changes

Long-term exposure of Mytilus galloprovincialis to diclofenac, Ibuprofen and Ketoprofen: Insights into bioavailability, biomarkers and transcriptomic changes

Toxicological assessment of anthropogenic Gadolinium in seawater: Biochemical effects in mussels Mytilus galloprovincialis

Revealing Mytilus galloprovincialis transcriptomic profiles during ontogeny

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