Transcriptomic dynamics provide an insight into the mechanism for silicon-mediated alleviation of salt stress in cucumber plants

Zhu, Yong‐Guan; Yin, Jia; Liang, Yan; Liu, Jiaqi; Jia, Jianhua; Huo, Heqiang; Wu, Zefeng; Yang, Ruolin; Gong, Haijun

doi:10.1016/j.ecoenv.2019.02.075

Cited by 68 publications

(54 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To confirm whether CsWRKYs are involved in the response to various stresses, we analysed the comprehensive expression patterns of CsWRKY genes under different abiotic and biotic stresses, including salt, heat, downy mildew (DM, Pseudoperonospora cubensis ) and powdery mildew (PM, Podosphaera fusca ), based on public transcriptome information [ 54 , 70 , 71 ] and transcriptomic data that we generated.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Genome-wide analysis of the WRKY gene family in the cucumber genome and transcriptome-wide identification of WRKY transcription factors that respond to biotic and abiotic stresses

Chen

Han

et al. 2020

BMC Plant Biol

View full text Add to dashboard Cite

Background Cucumber (Cucumis sativus L.) is an economically important vegetable crop species. However, it is susceptible to various abiotic and biotic stresses. WRKY transcription factors play important roles in plant growth and development, particularly in the plant response to biotic and abiotic stresses. However, little is known about the expression pattern of WRKY genes under different stresses in cucumber. Results In the present study, an analysis of the new assembly of the cucumber genome (v3.0) allowed the identification of 61 cucumber WRKY genes. Phylogenetic and synteny analyses were performed using related species to investigate the evolution of the cucumber WRKY genes. The 61 CsWRKYs were classified into three main groups, within which the gene structure and motif compositions were conserved. Tissue expression profiles of the WRKY genes demonstrated that 24 CsWRKY genes showed constitutive expression (FPKM > 1 in all samples), and some WRKY genes showed organ-specific expression, suggesting that these WRKYs might be important for plant growth and organ development in cucumber. Importantly, analysis of the CsWRKY gene expression patterns revealed that five CsWRKY genes strongly responded to both salt and heat stresses, 12 genes were observed to be expressed in response to infection from downy mildew and powdery mildew, and three CsWRKY genes simultaneously responded to all treatments analysed. Some CsWRKY genes were observed to be induced/repressed at different times after abiotic or biotic stress treatment, demonstrating that cucumber WRKY genes might play different roles during different stress responses and that their expression patterns vary in response to stresses. Conclusions Sixty-one WRKY genes were identified in cucumber, and insight into their classification, evolution, and expression patterns was gained in this study. Responses to different abiotic and biotic stresses in cucumber were also investigated. Our results provide a better understanding of the function of CsWRKY genes in improving abiotic and biotic stress resistance in cucumber.

show abstract

Section: Resultsmentioning

confidence: 99%

“…To investigate the potential functions of CsWRKYs in resistance to salt stress, we performed a CsWRKYs expression analysis after salt treatment based on public transcriptomic data [ 70 ] (Additional file 10 : Table S8). We observed that the expression levels of CsWRKY27, CsWRKY41 and CsWRKY50 considerably increased in response to salt stress.…”

Section: Resultsmentioning

confidence: 99%

Genome-wide analysis of the WRKY gene family in the cucumber genome and transcriptome-wide identification of WRKY transcription factors that respond to biotic and abiotic stresses

Chen

Han

et al. 2020

BMC Plant Biol

View full text Add to dashboard Cite

show abstract

“…These results suggested that CsWRKYs may play crucial roles in the response to various stresses. To further confirm whether CsWRKYs are involved in the response to various stresses, we analysed the comprehensive expression patterns of CsWRKY genes under different abiotic and biotic stresses, including salt, heat, downy mildew (DM, Pseudoperonospora cubensis) and powdery mildew (PM, Podosphaera fusca), based on public transcriptome information [54,60,61] and transcriptomic data that we generated.…”

Section: Expression Patterns Of Cswrkys In Response To Abiotic and Bimentioning

confidence: 99%

“…To investigate the potential functions of CsWRKYs in resistance to salt stress, we performed a CsWRKY expression analysis after salt treatment based on public transcriptomic data [60]. We observed that the expression levels of CsWRKY27, CsWRKY41 and CsWRKY50 considerably increased in response to salt stress.…”

Section: Expression Patterns Of Cswrkys In Response To Abiotic and Bimentioning

confidence: 99%

“…The expression regulation of CsWRKY genes responsive to different stresses was obtained from publicly available transcriptomic data, which were downloaded from Gene Expression Omnibus and analysed to reveal the genome-wide differentially expressed genes after treatment with salt (GSE116265) [60] and inoculation with DM (SRP009350) [54] and PM (GSE81234) [61]. Because the gene ID shown was according to the cucumber genome v2.0, we cross-referenced the gene IDs of the CsWRKYs with those of the cucumber genome v3.0.…”

Section: Transcriptome Analysis Of Cswrkys In Response To Abiotic Andmentioning

confidence: 99%

See 1 more Smart Citation

Genome-wide analysis of the WRKY gene family in the cucumber genome and transcriptome‑wide identification of WRKY transcription factors that respond to biotic and abiotic stresses

Chen

Han

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Cucumber ( Cucumis sativus L.) is an economically important vegetable crop species. However, it is susceptible to various abiotic and biotic stresses. WRKY transcription factors play important roles in plant growth and development, particularly in the plant response to biotic and abiotic stresses. However, little is known about the expression pattern of WRKY genes under different stresses in cucumber. Results: In the present study, an analysis of the new assembly of the cucumber genome (v3.0) allowed the identification of 61 cucumber WRKY genes. Phylogenetic and synteny analyses were performed using related species to investigate the evolution of the cucumber WRKY genes. The 61 CsWRKYs were classified into three main groups, within which the gene structure and motif compositions were conserved. Tissue expression profiles of the WRKY genes demonstrated that 24 CsWRKY genes showed constitutive expression (FPKM > 1 in all samples), and some WRKY genes showed organ-specific expression, suggesting that these WRKYs might be important for plant growth and organ development in cucumber. Importantly, analysis of the CsWRKY gene expression patterns revealed that 7 CsWRKY genes strongly responded to both salt and heat stresses, 12 genes were observed to be expressed in response to infection from downy mildew and powdery mildew, and three CsWRKY genes simultaneously responded to all treatments analysed. Some CsWRKY genes were observed to be induced/repressed at different times after abiotic or biotic stress treatment, demonstrating that cucumber WRKY genes might play different roles during different stress responses and that their expression patterns vary in response to stresses. Conclusions: Sixty-one WRKY genes were identified in cucumber, and insight into their classification, evolution, and expression patterns was gained in this study. Responses to different abiotic and biotic stresses in cucumber were also investigated. Our results provide a better understanding of the function of Cs WRKY genes in improving abiotic and biotic stress resistance in cucumber.

show abstract

Silicon and Plant Responses Under Adverse Environmental Conditions

Mundada

Jadhav²,

Salunkhe³

et al. 2021

Plant Performance Under Environmental Stress

View full text Add to dashboard Cite

Transcriptomic dynamics provide an insight into the mechanism for silicon-mediated alleviation of salt stress in cucumber plants

Cited by 68 publications

References 73 publications

Genome-wide analysis of the WRKY gene family in the cucumber genome and transcriptome-wide identification of WRKY transcription factors that respond to biotic and abiotic stresses

Genome-wide analysis of the WRKY gene family in the cucumber genome and transcriptome-wide identification of WRKY transcription factors that respond to biotic and abiotic stresses

Genome-wide analysis of the WRKY gene family in the cucumber genome and transcriptome‑wide identification of WRKY transcription factors that respond to biotic and abiotic stresses

Silicon and Plant Responses Under Adverse Environmental Conditions

Contact Info

Product

Resources

About