Transcriptomic comparison between two Vitis vinifera L. varieties (Trincadeira and Touriga Nacional) in abiotic stress conditions

Rocheta, Margarida; Coito, João L.; Ramos, Maria João; Carvalho, Luísa C.; Becker, Jörg; Carbonell‐Bejerano, Pablo; Amâncio, Sara

doi:10.1186/s12870-016-0911-4

Cited by 27 publications

(51 citation statements)

References 81 publications

(115 reference statements)

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“…Thus, there is a pressing need to understand the existing genetic diversity between and within varieties, and its influence on the physiological potential of the available genotypes to respond to climate changes and foster new terroirs. In light of this, an assembly of 65 differentially expressed genes (DEGs), known to respond to abiotic stresses in two well‐studied genotypes, ‘Touriga Nacional’ and ‘Trincadeira’ (Rocheta et al ), was designed to scan gene expression in leaves of 10 traditional Portuguese varieties growing in two Portuguese regions with distinct environmental conditions: one with a typical Mediterranean climate and the other with a marked Atlantic influence (Carvalho et al ). Due to the experimental set‐up behind the array design, the genotypes were characterized as ‘sensitive’ or ‘tolerant’ to abiotic stress and, furthermore, the DEGs were able to distinguish the main abiotic stress that each genotype/environment was subjected and responding to (drought, heat or excess light; Carvalho et al ).…”

Section: Abiotic Stress In the Field: Learning To Adjust To Climate Cmentioning

confidence: 99%

“…(e.g. Tattersall et al , Xiao et al , Liu et al , Rocheta et al , Carvalho et al ). The HSP20 family is known for a strong upregulation in response to abiotic stresses (Swindell et al ), so the high expression levels of HSP20 found by Liu et al () in leaves of ‘Cabernet Sauvignon’ and of HSP17.9 found by Carvalho et al () in leaves of ‘Touriga Nacional’ upon HS were in line with findings from other species.…”

Section: Controlled Conditions: Uncovering Stress Synergies and Antagmentioning

confidence: 99%

“…2). Also Rocheta et al (2016) reported downregulation of several WRKY TFs in leaves of 'Trincadeira' and 'Touriga Nacional' upon HS, while under UV-B stress some WRKY TFs were upregulated in leaves of 'Malbec' (Pontin et al 2010). In a greenhouse salt stress experiment involving the tolerant variety 'Razegui' and the susceptible variety 'Syrah', microarray analysis of leaves revealed several upregulated DEGs that included zinc finger TFs, ETHY-LENE RESPONSE FACTOR (ERFs) and a NAM-LIKE PRO-TEIN (NAC TF), with higher levels of upregulation in the tolerant 'Razegui' (Daldoul et al 2010).…”

Section: Transcriptomic Studies In Stress Response: the Relevance Of mentioning

confidence: 99%

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Cutting the Gordian Knot of abiotic stress in grapevine: From the test tube to climate change adaptation

Carvalho

Amâncio

2018

Physiologia Plantarum

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In Mediterranean climate areas, the available scenarios for climate change suggest an increase in the frequency of heat waves and severe drought in summer. Grapevine (Vitis vinifera L.) is a traditional Mediterranean species and is the most valuable fruit crop in the world. Currently, viticulture must adjust to impending climate changes that are already pushing vine‐growers toward the use of irrigation, with the concomitant losses in wine quality, and researchers to study tolerance to stress in existing genotypes. The viticulture and winemaking worlds are in demand to understand the physiological potential of the available genotypes to respond to climate changes. In this review, we will focus on the cross‐talk between common abiotic stresses that currently affect grapevine productivity and that are prone to affect it deeper in the future. We will discuss results obtained under three experimental stress conditions and that call for specific responses: (1) acclimatization of in vitro plantlets, (2) stress combinations in controlled conditions for research purposes, (3) extreme events in the field that, driven by climate changes, are pushing Mediterranean species to the limit. The different levels of tolerance to stress put in evidence by the plasticity of phenotypic and genotypic response mechanisms, will be addressed. This information is relevant to understand varietal adaptation to impending climate changes and to assist vine growers in choosing genotypes and viticulture practices.

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Section: Abiotic Stress In the Field: Learning To Adjust To Climate Cmentioning

confidence: 99%

Section: Controlled Conditions: Uncovering Stress Synergies and Antagmentioning

confidence: 99%

Section: Transcriptomic Studies In Stress Response: the Relevance Of mentioning

confidence: 99%

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Cutting the Gordian Knot of abiotic stress in grapevine: From the test tube to climate change adaptation

Carvalho

Amâncio

2018

Physiologia Plantarum

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“…To investigate the potential roles of the 35 identified grapevine genes in ABA and drought stress, two different grapevine-related databases for ABA (Pilati et al , 2017) and drought (Rocheta et al , 2016) stress treatments were analyzed. RNA-seq analysis was performed of grapevine berry skins with or without ABA treatment for 20 h and 44 h. A GrapeGene GeneChips ® data analysis was performed of leaves from two Vitis vinifera L. varieties (Trincadeira, TR and Touriga Nacional, TN) grown under control and drought greenhouse conditions, as well as fully irrigated and non-irrigated field conditions.…”

Section: Resultsmentioning

confidence: 99%

“…The heat maps were constructed using HemI software (Deng et al , 2014). To identify the predicted grapevine genes, two grapevine-related ABA (Pilati et al , 2017) and drought stress (Rocheta et al , 2016) databases were downloaded from the National Center for Biotechnology Information (NCBI) under BioProject accession number PRJNA369777 and the Gene Expression Omnibus (GEO) database under the number GSE57669.…”

Section: Methodsmentioning

confidence: 99%

VlbZIP30 of grapevine functions in drought tolerance via the abscisic acid core signaling pathway

Wang

Zhu

et al. 2017

Preprint

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Drought stress limits the growth and development of grapevines, thereby reducing productivity, but the mechanisms by which grapevines respond to drought stress remain largely uncharacterized. Here, we characterized a group A bZIP gene from ‘Kyoho’ grapevine, VlbZIP30, which was shown to be induced by abscisic acid (ABA) and dehydration stress. Overexpression of VlbZIP30 in transgenic Arabidopsis enhanced dehydration tolerance during seed germination, and in the seedling and adult stages. Transcriptome analysis revealed that a major proportion of ABA- and/or drought-responsive genes are transcriptionally regulated by VlbZIP30 during ABA or mannitol treatment at the cotyledon greening stage. We identified an A. thaliana G-box motif (CACGTG) and a potential grapevine G-box motif (MCACGTGK) in the promoters of the 39 selected A. thaliana genes up-regulated in the transgenic plants and in the 35 grapevine homologs, respectively. Subsequently, using two grapevine-related databases, we found that 74% and 84% (a total of 27 genes) of the detected grapevine genes were significantly up-regulated by ABA and drought stress, respectively, suggesting that these 27 genes involve in ABA or dehydration stress and may be regulated by VlbZIP30 in grapevine. We propose that VlbZIP30 functions as a positive regulator of drought-responsive signaling in the ABA core signaling pathway.HighlightVlbZIP30 positively regulate plant drought tolerance through regulated the expression of 27 grapevine candidate genes via G-box cis-element (MCACGTGK) in ABA signaling pathway.

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Insights into the cell-wall dynamics in grapevine berries during ripening and in response to biotic and abiotic stresses

Malacarne,

Lagreze,

Rojas San Martin

et al. 2024

Plant Mol Biol

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The cell wall (CW) is the dynamic structure of a plant cell, acting as a barrier against biotic and abiotic stresses. In grape berries, the modifications of pulp and skin CW during softening ensure flexibility during cell expansion and determine the final berry texture. In addition, the CW of grape berry skin is of fundamental importance for winemaking, controlling secondary metabolite extractability. Grapevine varieties with contrasting CW characteristics generally respond differently to biotic and abiotic stresses. In the context of climate change, it is important to investigate the CW dynamics occurring upon different stresses, to define new adaptation strategies. This review summarizes the molecular mechanisms underlying CW modifications during grapevine berry fruit ripening, plant-pathogen interaction, or in response to environmental stresses, also considering the most recently published transcriptomic data. Furthermore, perspectives of new biotechnological approaches aiming at modifying the CW properties based on other crops’ examples are also presented.

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Transcriptomic comparison between two Vitis vinifera L. varieties (Trincadeira and Touriga Nacional) in abiotic stress conditions

Cited by 27 publications

References 81 publications

Cutting the Gordian Knot of abiotic stress in grapevine: From the test tube to climate change adaptation

Cutting the Gordian Knot of abiotic stress in grapevine: From the test tube to climate change adaptation

VlbZIP30 of grapevine functions in drought tolerance via the abscisic acid core signaling pathway

Insights into the cell-wall dynamics in grapevine berries during ripening and in response to biotic and abiotic stresses

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