Transcriptomic changes in human umbilical cord blood endothelial cells under simulated microgravity

Rudimov, E. G.; Knjazev, E. N.; Khaustova, N. A.; Grigorieva, O. V.; Буравкова, Л. Б.

doi:10.1134/s160767291701001x

Cited by 12 publications

(3 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For bisulfite conversion using the innuCONVERT Bisulfite All-In-One Kit, 150 ng of isolated DNA were used. The DNA concentration after bisulfite conversion was determined using the ND-1000 Spectrophotometer (Thermo Fisher Scientific, Waltham, MA), as described previously [10,11].…”

Section: Dna Extraction and Bisulfite Conversionmentioning

confidence: 99%

The role of abnormal hypermethylation of the HOXA10 and HOXA11 promoters in implantation failures in IVF programs

Nazarenko

Kalinina

Knyazeva

et al. 2019

Gynecological Endocrinology

View full text Add to dashboard Cite

Objective: The objective of this study is to investigate the association between methylation levels of HOXA10 and HOXA11 promoters, clinical parameters, and implantation outcomes after in vitro fertilizationembryo transfer (IVT-ET) cycles in women with repeated implantation failures and tubal infertility. Methods: Endometrium samples were collected from 34 women during implantation window before IVF-ET cycle to assess methylation status of HOXA10 and HOXA11 promoters using bisulfite sequencing. All participants had a tubal factor of infertility and at least two implantation failures in the anamnesis. A logistic regression model was used to predict the implantation outcome depending on methylation status and clinical parameters. Results: The methylation of CpG-islands of HOXA10 and HOXA11 promoters was identified in 76.5 and 100% of participants, respectively. The median methylation levels did not differ significantly between the groups with different implantation outcomes, but a logistic regression model based on HOXA10 and HOXA11 methylation and clinical parameters allowed to classify the implantation outcomes with the total percentage of correct predictions of 85.19%. Conclusions: Abnormal methylation levels of the HOXA10 and HOXA11 promoters were found in the endometrium of women with tubal infertility and repeated implantation failures. The findings suggest that methylation status could be an important factor of implantation failure during IVF-ET cycles.

show abstract

Section: Dna Extraction and Bisulfite Conversionmentioning

confidence: 99%

The role of abnormal hypermethylation of the HOXA10 and HOXA11 promoters in implantation failures in IVF programs

Nazarenko

Kalinina

Knyazeva

et al. 2019

Gynecological Endocrinology

View full text Add to dashboard Cite

show abstract

“…11,12 RPM experiments performed by several groups, including our laboratory, discovered early and delayed changes in ECs. 8,[13][14][15][16][17][18][19] Under RPM conditions, EC growth is accompanied by the formation of unique threedimensional structures that resemble tubules and tissue-like aggregates on the endothelium surface. [20][21][22] These changes improve EC barrier function due to the elevated expression of tight junction proteins.…”

Section: Introductionmentioning

confidence: 99%

“…2D‐ and 3D‐clinorotation (generated by Random Positioning Machine (RPM) are the best methods for simulating the effects of microgravity (SMG) . RPM experiments performed by several groups, including our laboratory, discovered early and delayed changes in ECs . Under RPM conditions, EC growth is accompanied by the formation of unique three‐dimensional structures that resemble tubules and tissue‐like aggregates on the endothelium surface .…”

Section: Introductionmentioning

confidence: 99%

The ICAM‐1 expression level determines the susceptibility of human endothelial cells to simulated microgravity

Буравкова

Rudimov

Andreeva

et al. 2017

J of Cellular Biochemistry

View full text Add to dashboard Cite

Microgravity is a principal risk factor hampering human cardiovascular regulation during space flights. Endothelial dysfunction associated with the impaired integrity and uniformity of the monolayer represents a potential trigger for vascular damage. We characterized the expression profile of the multi-step cascade of adhesion molecules (ICAM-1, VCAM-1, E-selectin, VE-cadherin) in umbilical cord endothelial cells (ECs) after 24 h of exposure to simulated microgravity (SMG), pro-inflammatory cytokine TNF-α, and the combination of the two. Random Positioning Machine (RPM)-mediated SMG was used to mimic microgravity effects. SMG stimulated the expression of E-selectin, which is known to be involved in slowing leukocyte rolling. Primary ECs displayed heterogeneity with respect to the proportion of ICAM-1-positive cells. ECs were divided into two groups: pre-activated ECs displaying high proportion of ICAM-1 -cells (ECs-1) (greater than 50%) and non-activated ECs with low proportion of ICAM-1 -cells (ECs-2) (less than 25%). Only non-activated ECs-2 responded to SMG by elevating gene transcription and increasing ICAM-1 and VE-cadherin expression. This effect was enhanced after cumulative SMG-TNF-α exposure. ECs-1 displayed an unexpected decrease in number of E-selectin- and ICAM-1-positive ECs and pronounced up-regulation of VCAM1 upon activation of inflammation, which was partially abolished by SMG. Thus, non-activated ECs-2 are quite resistant to the impacts of microgravity and even exhibited an elevation of the VE-cadherin gene and protein expression, thus improving the integrity of the endothelial monolayer. Pre-activation of ECs with inflammatory stimuli may disturb the EC adhesion profile, attenuating its barrier function. These alterations may be among the mechanisms underlying cardiovascular dysregulation in real microgravity conditions.

show abstract

Expression of microRNA Genes MIR221, MIR222, and MIR181B1 Increases during Induction of Inflammation in the Endothelial Barrier Model

et al. 2018

View full text Add to dashboard Cite

We studied expression profile of microRNA and their target genes in human umbilical vein endothelial cells (HUVEC) during proinflammatory activation with TNFα. TNFα-induced activation of HUVEC was accompanied by a decrease in the expression of CDKN1B, HIST1H3D, and OIP5 genes that are the common target genes for mature microRNA encoded by MIR221, MIR222, and MIR181B1 genes, whose expression increases in activated cells. Proteins encoded by HIST1H3D and OIP5 genes are associated with chromatin compaction and cell cycle. Our results suggest that fetal endothelial microRNA can appear in the maternal blood during various pathological states, e.g., under conditions of preeclampsia.

show abstract

Transcriptomic changes in human umbilical cord blood endothelial cells under simulated microgravity

Cited by 12 publications

References 8 publications

The role of abnormal hypermethylation of the HOXA10 and HOXA11 promoters in implantation failures in IVF programs

The role of abnormal hypermethylation of the HOXA10 and HOXA11 promoters in implantation failures in IVF programs

The ICAM‐1 expression level determines the susceptibility of human endothelial cells to simulated microgravity

Expression of microRNA Genes MIR221, MIR222, and MIR181B1 Increases during Induction of Inflammation in the Endothelial Barrier Model

Contact Info

Product

Resources

About