Transcriptomic basis for drought-resistance in Brassica napus L.

Abstract

Based on transcriptomic data from four experimental settings with drought-resistant and drought-sensitive cultivars under drought and well-watered conditions, statistical analysis revealed three categories encompassing 169 highly differentially expressed genes (DEGs) in response to drought in Brassica napus L., including 37 drought-resistant cultivar-related genes, 35 drought-sensitive cultivar-related genes and 97 cultivar non-specific ones. We provide evidence that the identified DEGs were fairly uniformly distributed on different chromosomes and their expression patterns are variety specific. Except commonly enriched in response to various stimuli or stresses, different categories of DEGs show specific enrichment in certain biological processes or pathways, which indicated the possibility of functional differences among the three categories. Network analysis revealed relationships among the 169 DEGs, annotated biological processes and pathways. The 169 DEGs can be classified into different functional categories via preferred pathways or biological processes. Some pathways might simultaneously involve a large number of shared DEGs, and these pathways are likely to cross-talk and have overlapping biological functions. Several members of the identified DEGs fit to drought stress signal transduction pathway in Arabidopsis thaliana. Finally, quantitative real-time PCR validations confirmed the reproducibility of the RNA-seq data. These investigations are profitable for the improvement of crop varieties through transgenic engineering.

Sugar transporter proteins in Capsicum: identification, characterization, evolution and expression patterns

“…The plant growth and development are seriously affected by abiotic stress [44][45][46], including high temperature and low temperature [47][48][49][50][51][52][53][54][55][56][57]. In the present study, based on available genome-wide RNA-seq data, we analyzed the expression profile of pepper STP genes under cold and high temperature stress treatments.…”

Method for Determining CYP2C9 Activity in Earthworms and its Responses to Benzo[a]pyrene or Pyrene in Soil

“…The individual B[a]P or pyrene exposure experiments were conducted in a manner similar to those described previously by Yang et al, and the doses of contaminants were also selected based on previous work in which PAHs pollution in sewage irrigation of Shenyang, China, was investigated . A certain concentration of pyrene‐ or B[a]P‐acetone solution was thoroughly mixed with 500 g soil in an aerated container (15 × 10 × 12 cm 3 ), and earthworms were thus exposed to soils with increasing doses of individual pyrene or B[a]P (0.12, 0.24, 0.48, and 0.96 mg kg −1 soil) except for the control soil.…”

Genome-wide analysis of long non-coding RNAs (lncRNAs) in two contrasting rapeseed (Brassica napus L.) genotypes subjected to drought stress

“…ABA binds its receptor PYR/PYL/RCAR (pyrabactin resistant/PYR-like/regulatory component of ABA) and inhibits the activity of PP2C (protein phosphatases type-2C), which leads to the autophosphorylation of downstream SnRK2 (sucrose non-fermenting 1-related subfamily 2 kinases) and the phosphorylation of downstream ABF transcription factors, regulating the expression of stress-related genes [80,81]. BnaC07g44670D is homologous to gene ABF (AT4G34000) in Arabidopsis thaliana, which has been reported to be an important gene involved in ABA signaling [82].…”