Transcriptomic and proteomic effects of a neonicotinoid insecticide mixture in the marine mussel (Mytilus galloprovincialis, Lam.)

Dondero, Francesco; Negri, Alessandro; Boatti, Lara; Marsano, Francesco; Mignone, Flavio; Viarengo, Aldo

doi:10.1016/j.scitotenv.2010.03.040

Cited by 80 publications

(56 citation statements)

References 53 publications

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“…We assessed whether exposure to clothianidin enhanced the expression of Dmel\LRR and the NF-κB inhibitory pathway under its control, as observed in M. galloprovincialis for related NLR (CLR) genes (25). The level of NF-κB activation was determined by monitoring the expression of the drosomycin-GFP reporter gene that is under the control of the Toll pathway (32) in a transgenic strain of D. melanogaster exposed by topical treatment to a standard LD 50 dose of the neonicotinoid clothianidin (40 ng per larva, Fig.…”

Section: Resultsmentioning

confidence: 99%

“…For D. melanogaster, the toxicity of the insecticides clothianidin and chlorpyriphos was assessed on third instar larvae. Groups of 30 experimental larvae were treated with the following doses per individual: 1.5, 3,6,12,25,50,70, and 100 ng of clothianidin; 2.5, 5, 15, 60, 125, and 250 ng of chlorpyriphos. Treated larvae were kept for 24 h at 21°C and 60% RH in an incubator with food available, and their survival rate was then assessed.…”

Section: Methodsmentioning

confidence: 99%

“…Ecotoxicological studies have reported that exposure of Mytilus galloprovincialis to sublethal doses of the neonicotinoid insecticide thiacloprid up-regulates the expression of transcription factors that show sequence similarity with members of the CAT-ERPILLER (CLR) protein family in mammals (25), subsequently renamed, along with other aliases, as "Nucleotide-binding domain and Leucine-rich Repeat" (NLR) (26). These proteins play an Significance Honey bees are exposed to a wealth of synergistically interacting stress factors, which may induce colony losses often associated with high infection levels of pathogens.…”

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confidence: 99%

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Neonicotinoid clothianidin adversely affects insect immunity and promotes replication of a viral pathogen in honey bees

Prisco

Cavaliere

Annoscia

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

554

445

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Large-scale losses of honey bee colonies represent a poorly understood problem of global importance. Both biotic and abiotic factors are involved in this phenomenon that is often associated with high loads of parasites and pathogens. A stronger impact of pathogens in honey bees exposed to neonicotinoid insecticides has been reported, but the causal link between insecticide exposure and the possible immune alteration of honey bees remains elusive. Here, we demonstrate that the neonicotinoid insecticide clothianidin negatively modulates NF-κB immune signaling in insects and adversely affects honey bee antiviral defenses controlled by this transcription factor. We have identified in insects a negative modulator of NF-κB activation, which is a leucine-rich repeat protein. Exposure to clothianidin, by enhancing the transcription of the gene encoding this inhibitor, reduces immune defenses and promotes the replication of the deformed wing virus in honey bees bearing covert infections. This honey bee immunosuppression is similarly induced by a different neonicotinoid, imidacloprid, but not by the organophosphate chlorpyriphos, which does not affect NF-κB signaling. The occurrence at sublethal doses of this insecticide-induced viral proliferation suggests that the studied neonicotinoids might have a negative effect at the field level. Our experiments uncover a further level of regulation of the immune response in insects and set the stage for studies on neural modulation of immunity in animals. Furthermore, this study has implications for the conservation of bees, as it will contribute to the definition of more appropriate guidelines for testing chronic or sublethal effects of pesticides used in agriculture.Apis mellifera | DWV | NLR (CLR) | neuroimmunity | toxicology

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Neonicotinoid clothianidin adversely affects insect immunity and promotes replication of a viral pathogen in honey bees

Prisco

Cavaliere

Annoscia

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

554

445

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“…The authors reported a site and organ-specific response to the pesticides. Effects of imidacloprid and thiacloprid, 2 neonicotinoid insecticides, at transcriptomic and proteomic levels in the marine mussel, Mytilus galloprovincialis, were also reported by Dondero et al (2010). Tlili et al (2010) compared the size-distribution of the intra-sedimentary bivalve Donax trunculus collected in two sites in Tunisia, a polluted site and a comparatively reference site The auhors showed that the size-distribution from the polluted site consisted of 4 cohorts.whereras 5 cohorts were observed in the comparatively reference site.…”

Section: Other Effects Of Pesticides On Marine Bivalvesmentioning

confidence: 95%

Effects of Pesticides on Marine Bivalves: What Do We Know and What Do We Need to Know?

Renault¹

2011

Pesticides in the Modern World - Risks and Benefits

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“…Many studies have shown the oxidative and neurotoxic potential of IMI in a variety of animal species, including rats (12)(13)(14)(15)(16)(17)(18)(19). However, none has investigated how biotransformation affects prooxidative and neurotoxic effects of IMI in mammals and whether it depends on sex, tissue, or exposure duration.…”

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confidence: 99%

Sex-, tissue-, and exposure duration-dependent effects of imidacloprid modulated by piperonyl butoxide and menadione in rats. Part I: oxidative and neurotoxic potentials

Yardimci

Sevgiler

Rencüzoğulları

et al. 2014

Archives of Industrial Hygiene and Toxicology

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Earlier research has evidenced the oxidative and neurotoxic potential of imidacloprid, a neonicotinoid insecticide, in different animal species. The primary aim of this study was to determine how metabolic modulators piperonyl butoxide and menadione affect imidacloprid's adverse action in the liver and kidney of Sprague-Dawley rats of both sexes. The animals were exposed to imidacloprid alone (170 mg kg -1 ) or in combination with piperonyl butoxide (100 mg kg -1 ) or menadione (25 mg kg -1 ) for 12 and 24 h. Their liver and kidney homogenates were analysed spectrophotometrically for glutathione peroxidase, glutathione S-transferase, catalase, total cholinesterase specific activities, total glutathione, total protein content, and lipid peroxidation levels. Imidacloprid displayed its prooxidative and neurotoxic effects predominantly in the kidney of male rats after 24 h of exposure. Our findings suggest that the observed differences in prooxidative and neurotoxic potential of imidacloprid could be related to differences in its metabolism between the sexes. Co-exposure (90-min pre-treatment) with piperonyl butoxide or menadione revealed tissue-specific effect of imidacloprid on total cholinesterase activity. Increased cholinesterase activity in the kidney could be an adaptive response to imidacloprid-induced oxidative stress. In the male rat liver, co-exposure with piperonyl butoxide or menadione exacerbated imidacloprid toxicity. In female rats, imidacloprid+menadione co-exposure caused prooxidative effects, while no such effects were observed with imidacloprid alone or menadione alone. In conclusion, sex-, tissue-, and duration-specific effects of imidacloprid are remarkable points in its toxicity.

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Transcriptomic and proteomic effects of a neonicotinoid insecticide mixture in the marine mussel (Mytilus galloprovincialis, Lam.)

Cited by 80 publications

References 53 publications

Neonicotinoid clothianidin adversely affects insect immunity and promotes replication of a viral pathogen in honey bees

Neonicotinoid clothianidin adversely affects insect immunity and promotes replication of a viral pathogen in honey bees

Effects of Pesticides on Marine Bivalves: What Do We Know and What Do We Need to Know?

Sex-, tissue-, and exposure duration-dependent effects of imidacloprid modulated by piperonyl butoxide and menadione in rats. Part I: oxidative and neurotoxic potentials

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