Transcriptomic and Proteomic Analyses of a New Cytoplasmic Male Sterile Line with wildGossypium bickii.Genetic Background

Abstract

39Cotton is an important fiber crop but has serious effects of heterosis, in which cytoplasmic male 40 sterility (CMS) being the major cause of heterosis in plants. However, there are no studies done on 41 CMS Yamian A in cotton with the genetic background of the Australian wild Gossypium bickii. 42 Transcriptomic and proteomic results showed that UDP-glucosyltransferase -in the nucleus, 60S 43 ribosomal protein L13a-in the cytoplasm, ribulose-1,5-bisphosphate carboxylase/oxygenase -in the 44 chloroplast, glutathione S-transferase -in the cytoplasm, and ATP synthase F1 subunit 1 -in the 45 mitochondrion were upregulated; while low molecular weight heat shock protein -in the chloroplast 46 and ATP synthase D chain-in the mitochondrion were down-regulated expression at the microspore 47 58 Cotton is an important cash crop with high-quality fiber, edible oil, and protein which is mainly 59 used as animal feeds (1) . Heterosis in cotton is quite apparent and has been widely used in yield quality, 60 and resistance studies in cotton (2) . The adoption of the production of hybrid seeds is the most 61 important link among some links of cotton heterosis use. At present, the castration in the production 62 of hybrid seeds often relies on hand emasculation and male-sterile lines including chemically induced 63 male sterility (IMS), genic male sterility (GMS), and cytoplasmic male sterility (CMS) (3) . The 64 production practice showed that CMS is an effective way of heterosis utilization in crop and widely 65 used to produce hybrid seeds, because it eliminates the need for artificial emasculation, saves 66 manpower and material resources, enhance the purity of hybrid seeds and increases the output of 67 crops (3, 4) . All over the world, cotton studies started in the 1960s, since then a number of germplasms 68 have been developed, such as G. arboreum L, G. harknessii Brandegee, G. trilobum (DC.) Skov., G. 69 hirsutum, G. barbadense L., among others. However, there is no report on CMS in cotton with 70 genetic background of Australian wild Gossypium bickii, which has been reported despite the 71 enormous effects of heterosis in cotton germplasm development. 72 In recent years, advancement in molecular technology, has enabled breeders and molecular 73 researchers to identify various plant transcription factors, genes and explore protein expression at the 74 translational, transcriptome and proteome level, such as the CMS studies of the Chinese cabbage (5) , 75 turnip (6) , Cucumis melo L. (7) , cotton (8,9) , rice (10) , Brassica napus L. (11) . Transcriptomic analysis in 76 5 cotton (CMS-D8) revealed that the reactive oxygen species (ROS) were released from mitochondria 77 and served as important signal molecules in the nucleus, causing the formation of abnormal 78 tapetum (8) . Proteome analyses in cotton indicated that the differentially expressed proteins (DEPs) 79 mainly involved in pyruvate, carbohydrate and fatty acid metabolism had been identified between the 80 male-sterile line 1355A a...

Crotonylation versus acetylation in petunia corollas with reduced acetyl‐CoA due to PaACL silencing

“…To obtain PaACLB-silenced plants, A. tumefaciens carrying pTRV2 empty vector and the previously constructed pTRV2-PaACLB1-B2 vector (Zhao, Wang, et al, 2020;Zhao, Zhong, et al, 2020) and S1), which was consistent with our previous results (Zhao, Wang, et al, 2020;Zhao, Zhong, et al, 2020).…”

“…These results showed that crotonylation had a closer relationship with acetylation than with ubiquitination. pathway (Zhao, Wang, et al, 2020;Zhao, Zhong, et al, 2020). In the present study, proteins with different crotonylation sites were also enriched in the TCA cycle pathway.…”

“…Trypsin digestion was performed according to our previous protocol (Zhao, Wang, et al, 2020;Zhao, Zhong, et al, 2020). Then, 5 mM dithiothreitol was used to reduce the protein solution for 30 min at 56 C. The protein solution was alkylated with 11 mM iodoacetamide at room temperature for 15 min in the dark and was then diluted by adding 100 mM triethylammonium bicarbonate (TEAB) to a urea concentration less than 2 M. Protein digestion is divided into two steps.…”

Transcriptome profiling of flower buds of male-sterile lines provides new insights into male sterility mechanism in alfalfa

“…Lower content of ribosomal protein during anther abortion was observed in cotton, suggesting that insufficient synthesis of ribosomal protein may have hindered pollen production [ 47 ]. However, Zhao et al [ 48 ] found that the 60S ribosomal protein L13a-4-like was up-regulated in the male sterile line and hence was related to the abortion of microspores in cotton. These results indicate that the up- or down-regulation of some key ribosomal proteins may lead to male sterility.…”

Integrated cyto-physiological and proteomic analyses reveal new insight into CMS mechanism in a novel upland cotton CMS line LD6A

“…In cotton GMS line 2074A, GhA11G1250 encoded a mitochondrial localization of peroxisomal-like protein HSP14.5, participated in response to ROS is critical to pollen abortion (Nie et al, 2020). UDP-glucuronosyl/UDP-glucosyltransferase, 60S ribosomal protein L13a-4-like, and glutathione S-transferase, as well as heat shock protein Hsp20, ATPase, F0 complex, and subunit D were related to the microspore abortion in Yamian A, a CMS line with a wild cotton genetic background (Zhao et al, 2020).…”