Transcriptomic and physiological comparsion of the short-term responses of two Oryza sativa L. varieties to cadmium

Chen, Jiahui; Wu, Xue; Song, Jinxue; Xing, Guipei; Liang, Lu; Yin, Qianlan; Guo, Aijing; Chen, Jin

doi:10.1016/j.envexpbot.2020.104292

Cited by 18 publications

(6 citation statements)

References 61 publications

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“…To verify the reliability of the transcriptome results, 8 DEGs co-expressed in B116-CK, B116-T, B144-CK, and B144-T were selected, and the primers were designed using SnapGene 3.2.1 (the sequences are listed in Supplementary Table S1 ) and were synthesized by Tsingke Biotechnology Co., Ltd. (Beijing, China) before then being synthesized using the ChamQ Universal SYBR ® qPCR Master Mix Q711 (Vazyme Biotech Co., Ltd., Nanjing, China). The RNA used for quantitative RT-PCR was the same as that used to construct the cDNA library, and the related genes were normalized to Actin transcript levels by the 2 −ΔΔCt method [ 37 ].…”

Section: Methodsmentioning

confidence: 99%

Physiological and Transcriptomic Analyses Reveal the Mechanisms of Compensatory Growth Ability for Early Rice after Low Temperature and Weak Light Stress

Wang

Zhong

et al. 2022

Plants

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“Late spring coldness” (T) is a frequent meteorological disaster in the spring in southern China, often causing severe yield losses of direct-seeded early rice. In this study, we investigated the mechanisms underlying the differences in the compensatory growth ability of different rice genotypes by focusing on agronomic traits, physiological indicators, and transcriptome. The results showed that there were significant differences in the compensatory growth recovery ability of different genotypes after a combination of four days of low temperature and weak light stress. Only the strong compensatory growth genotype B116 was able to grow rapidly and reduce soluble protein and H2O2 concentrations rapidly after stress. By analyzing enzyme activity as well as endogenous hormone concentration, we found that the high superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activities and high levels of abscisic acid (ABA) could reduce the damage of B116 during stress. Meanwhile, higher glutamine synthetase (GS) and nitrate reductase (NR) activity and higher levels of gibberellin A3(GA3), indoleacetic acid (IAA), and zeatin nucleoside (ZR) could enable B116 to grow rapidly after stress. The identified differentially expressed genes (DEGs) indicated that there were large differences in POD-related genes and gibberellin metabolism between B116 and B144 after stress; RT-PCR quantification also showed a trend consistent with RNA-seq, which may be an important reason for the differences in compensatory growth ability.

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Section: Methodsmentioning

confidence: 99%

Physiological and Transcriptomic Analyses Reveal the Mechanisms of Compensatory Growth Ability for Early Rice after Low Temperature and Weak Light Stress

Wang

Zhong

et al. 2022

Plants

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“…We slightly modified the method of Chen et al (2021) [ 77 ]: First, the roots, stems, and leaves were washed with 20 mM EDTA-Na 2 solution for 30 min, and then with deionized water three to five times. The washed samples were dried at 105 °C for 25 min, and then at 80 °C until they were completely dry.…”

Section: Methodsmentioning

confidence: 99%

Spraying Zinc Sulfate to Reveal the Mechanism through the Glutathione Metabolic Pathway Regulates the Cadmium Tolerance of Seashore Paspalum (Paspalum vaginatum Swartz)

Cui

Chen

et al. 2023

Plants

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Cadmium (Cd) is considered to be one of the most toxic metals, causing serious harm to plants’ growth and humans’ health. Therefore, it is necessary to study simple, practical, and environmentally friendly methods to reduce its toxicity. Until now, people have applied zinc sulfate to improve the Cd tolerance of plants. However, related studies have mainly focused on physiological and biochemical aspects, with a lack of in-depth molecular mechanism research. In this study, we sprayed high (40 mM) and low (2.5 mM) concentrations of zinc sulfate on seashore paspalum (Paspalum vaginatum Swartz) plants under 0.5 mM Cd stress. Transcriptome sequencing and physiological indicators were used to reveal the mechanism of Cd tolerance. Compared with the control treatment, we found that zinc sulfate decreased the content of Cd2+ by 57.03–73.39%, and that the transfer coefficient of Cd decreased by 58.91–75.25% in different parts of plants. In addition, our results indicate that the antioxidant capacity of plants was improved, with marked increases in the glutathione content and the activity levels of glutathione reductase (GR), glutathione S-transferase (GST), and other enzymes. Transcriptome sequencing showed that the differentially expressed genes in both the 0.5 Zn and 40 Zn treatments were mainly genes encoding GST. This study suggests that genes encoding GST in the glutathione pathway may play an important role in regulating the Cd tolerance of seashore paspalum. Furthermore, the present study provides a theoretical reference for the regulation mechanism caused by zinc sulfate spraying to improve plants’ Cd tolerance.

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“…The culture medium was replaced every 3 d. Subsequently, the plants were transferred into two hydroponic boxes (l × w × h: 37.5 cm × 25.0 cm × 13.5 cm; 12 seedlings per box) with 10 L 1/2 modified Hoagland nutrient solution (renewed every 3 d). One month later, seedlings in one box were treated with 50 μM Cd 2+ (via CdCl 2 •2.5H 2 O), an appropriate Cd concentration that can effectively trigger changes in plant gene expression in a short time ( Chen et al., 2021 ; Wang et al., 2022 ), whereas those in the other remained untreated as controls.…”

Section: Methodsmentioning

confidence: 99%

Rhizospheric microbiomics integrated with plant transcriptomics provides insight into the Cd response mechanisms of the newly identified Cd accumulator Dahlia pinnata

Jin

et al. 2022

Front. Plant Sci.

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Phytoremediation that depends on excellent plant resources and effective enhancing measures is important for remediating heavy metal-contaminated soils. This study investigated the cadmium (Cd) tolerance and accumulation characteristics of Dahlia pinnata Cav. to evaluate its Cd phytoremediation potential. Testing in soils spiked with 5–45 mg kg–1 Cd showed that D. pinnata has a strong Cd tolerance capacity and appreciable shoot Cd bioconcentration factors (0.80–1.32) and translocation factors (0.81–1.59), indicating that D. pinnata can be defined as a Cd accumulator. In the rhizosphere, Cd stress (45 mg kg–1 Cd) did not change the soil physicochemical properties but influenced the bacterial community composition compared to control conditions. Notably, the increased abundance of the bacterial phylum Patescibacteria and the dominance of several Cd-tolerant plant growth–promoting rhizobacteria (e.g., Sphingomonas, Gemmatimonas, Bryobacter, Flavisolibacter, Nocardioides, and Bradyrhizobium) likely facilitated Cd tolerance and accumulation in D. pinnata. Comparative transcriptomic analysis showed that Cd significantly induced (P < 0.001) the expression of genes involved in lignin synthesis in D. pinnata roots and leaves, which are likely to fix Cd2+ to the cell wall and inhibit Cd entry into the cytoplasm. Moreover, Cd induced a sophisticated signal transduction network that initiated detoxification processes in roots as well as ethylene synthesis from methionine metabolism to regulate Cd responses in leaves. This study suggests that D. pinnata can be potentially used for phytoextraction and improves our understanding of Cd-response mechanisms in plants from rhizospheric and molecular perspectives.

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Transcriptomic and physiological comparsion of the short-term responses of two Oryza sativa L. varieties to cadmium

Cited by 18 publications

References 61 publications

Physiological and Transcriptomic Analyses Reveal the Mechanisms of Compensatory Growth Ability for Early Rice after Low Temperature and Weak Light Stress

Physiological and Transcriptomic Analyses Reveal the Mechanisms of Compensatory Growth Ability for Early Rice after Low Temperature and Weak Light Stress

Spraying Zinc Sulfate to Reveal the Mechanism through the Glutathione Metabolic Pathway Regulates the Cadmium Tolerance of Seashore Paspalum (Paspalum vaginatum Swartz)

Rhizospheric microbiomics integrated with plant transcriptomics provides insight into the Cd response mechanisms of the newly identified Cd accumulator Dahlia pinnata

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