Transcriptomic and phenotypic analysis of murine embryonic stem cell derived BMP2+ lineage cells: an insight into mesodermal patterning

Abstract

Transcriptome and phenotype of BMP2 + cells <p>Transcriptome analysis of BMP2⁺cells in comparison to the undifferentiated BMP2 ES cells and the control population from 7-day old embryoid bodies led to the identification of 479 specifically upregulated and 193 downregulated transcripts.</p> Abstract Background: Bone morphogenetic protein (BMP)2 is a late mesodermal marker expressed during vertebrate development and plays a crucial role in early embryonic development. The nature of the BMP2-expressing cells during the early stages of embryonic development, their transcriptome and cell phenotypes developed from these cells have not yet been characterized.

KAP1 Represses Differentiation-Inducible Genes in Embryonic Stem Cells through Cooperative Binding with PRC1 and Derepresses Pluripotency-Associated Genes

“…We compared the changes in ES cell transcription observed upon KAP1 fl/fl knockout with the changes in transcription associated with a variety of developmental transitions and signaling responses reported previously. We observed a close correspondence between the changes in ES cell transcription upon KAP1 fl/fl knockout and upon embryoid body formation (49) (Fig. 3).…”

Dexamethasone modulates BMP‐2 effects on mesenchymal stem cells in vitro

“…Besides species-related and individual differences, it is evident that BMP-2 regulates not only osteoblast differentiation but also influences other pathways of different human cell types. [41][42][43][44] It also should be considered that glucocorticoids influence the recruitment and differentiation of other bone cells such as osteoclasts, as shown by Defranco et al, 45 and also that a large number of genes respond to BMP-2 stimulus. 46 In our study, we did not evaluate these boneresorbing cells, but we did observe an increased number of dead cells in DAG þ BMP-2-stimulated cultures (X: 12.1%) compared to DAGþ (6.36%) and control cultures (10.98%).…”

Unique Metabolic Features of Stem Cells, Cardiomyocytes, and Their Progenitors

“…Moreover, a set of differentiated cardiovascular cell types has been derived from ESCs (Figures 3 and 4). 13,[77][78][79][80][81][82] Figure 3. Statistical determination of differentially expressed genes from the transcriptome data set of murine embryonic stem cells (ESCs) randomly differentiated into 10-day old embryoid bodies (EBs) (time kinetic, random differentiation).…”