Transcriptomic and metabolomic investigation of the adaptation mechanisms of blueberries to nitrogen deficiency stress

Yang, Haiyan; Wei, Zhiwen; Duan, Yongkang; Wu, Yaqiong; Zhang, Chunhong; Wang, Wenlong; Lyu, Lianfei; Li, Weilin

doi:10.1016/j.scienta.2023.112376

Cited by 5 publications

(3 citation statements)

References 38 publications

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“…The two phases of plant photosynthesis are known as the light and carbon reactions. The light reaction in higher plants is mainly caused by the cooperation of four transmembrane multi-subunit protein complexes located in the thylakoid membrane of the chloroplast: PSII, cytochrome b6f, PSI, and ATP synthase [30,31]. The unstable chemical energy (ATP and NADPH) generated via the light reaction is transformed by the carbon reaction into more stable forms that are kept in glucose molecules [32,33].…”

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptome Analysis Reveals the Protective Role of Melatonin during Salt Stress by Regulating the Photosynthesis and Ascorbic Acid Metabolism Pathways in Brassica campestris

Shah,

Wang,

et al. 2024

IJMS

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Salinity stress is a type of abiotic stress which negatively affects the signaling pathways and cellular compartments of plants. Melatonin (MT) has been found to be a bioactive compound that can mitigate these adverse effects, which makes it necessary to understand the function of MT and its role in salt stress. During this study, plants were treated exogenously with 100 µM of MT for 7 days and subjected to 200 mM of salt stress, and samples were collected after 1 and 7 days for different indicators and transcriptome analysis. The results showed that salt reduced chlorophyll contents and damaged the chloroplast structure, which was confirmed by the downregulation of key genes involved in the photosynthesis pathway after transcriptome analysis and qRT-PCR confirmation. Meanwhile, MT increased the chlorophyll contents, reduced the electrolyte leakage, and protected the chloroplast structure during salt stress by upregulating several photosynthesis pathway genes. MT also decreased the H2O2 level and increased the ascorbic acid contents and APX activity by upregulating genes involved in the ascorbic acid pathway during salt stress, as confirmed by the transcriptome and qRT-PCR analyses. Transcriptome profiling also showed that 321 and 441 DEGs were expressed after 1 and 7 days of treatment, respectively. The KEGG enrichment analysis showed that 76 DEGs were involved in the photosynthesis pathway, while 35 DEGs were involved in the ascorbic acid metabolism pathway, respectively. These results suggest that the exogenous application of MT in plants provides important insight into understanding MT-induced stress-responsive mechanisms and protecting Brassica campestris against salt stress by regulating the photosynthesis and ascorbic acid pathway genes.

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Section: Discussionmentioning

confidence: 99%

Comparative Transcriptome Analysis Reveals the Protective Role of Melatonin during Salt Stress by Regulating the Photosynthesis and Ascorbic Acid Metabolism Pathways in Brassica campestris

Shah,

Wang,

et al. 2024

IJMS

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“…Sugar is not only an energy source and an important component of plant structural substances; it also acts as a signal molecule that regulates various metabolic activities in plants in conjunction with signals such as hormones and nitrogen, and it participates in the expression of related genes via signal transduction [ 13 , 15 , 50 ]. According to studies, auxin and BR are hormones with obvious links to sugar sensing and signaling.…”

Section: Discussionmentioning

confidence: 99%

“…Sugar is the main component of fruit quality and flavor, as well as the primary raw material for vitamin, aromatic compound, and pigment synthesis [ 11 , 12 ]. Recent studies have demonstrated that sugars can be employed as signaling molecules to participate in the metabolic processes of hormones, anthocyanins, nitrogen, and other substances [ 13 , 14 , 15 ]. As a result, in-depth research into the carbohydrate accumulation mechanism in the fruit is the foundation of the quality formation theory, and it is critical to increasing the quality of blueberry fruit.…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic and Metabolomic Profiling Reveals the Variations in Carbohydrate Metabolism between Two Blueberry Cultivars

Yang,

Wei,

et al. 2023

IJMS

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Blueberry is a high-quality fruit tree with significant nutritional and economic value, but the intricate mechanism of sugar accumulation in its fruit remains unclear. In this study, the ripe fruits of blueberry cultivars ‘Anna’ and ‘Misty’ were utilized as experimental materials, and physiological and multi-omics methodologies were applied to analyze the regulatory mechanisms of the difference in sugar content between them. The results demonstrated that the ‘Anna’ fruit was smaller and had less hardness than the ‘Misty’ fruit, as well as higher sugar content, antioxidant capability, and lower active substance content. A total of 7067 differentially expressed genes (DEGs) (3674 up-regulated and 3393 down-regulated) and 140 differentially abundant metabolites (DAMs) (82 up-regulated and 58 down-regulated) were identified between the fruits of the two cultivars. According to KEGG analysis, DEGs were primarily abundant in phenylpropanoid synthesis and hormone signal transduction pathways, whereas DAMs were primarily enriched in ascorbate and aldarate metabolism, phenylpropanoid biosynthesis, and the pentose phosphate pathway. A combined multi-omics study showed that 116 DEGs and 3 DAMs in starch and sucrose metabolism (48 DEGs and 1 DAM), glycolysis and gluconeogenesis (54 DEGs and 1 DAM), and the pentose phosphate pathway (14 DEGs and 1 DAM) were significantly enriched. These findings suggest that blueberries predominantly increase sugar accumulation by activating carbon metabolism network pathways. Moreover, we identified critical transcription factors linked to the sugar response. This study presents new understandings regarding the molecular mechanisms underlying blueberry sugar accumulation and will be helpful in improving blueberry fruit quality through breeding.

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Identification of crucial metabolites in colored grain wheat (Triticum aestivum L.) regulated by nitrogen application

Yan,

Zhang,

Jia

et al. 2024

Food Research International

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Transcriptomic and metabolomic investigation of the adaptation mechanisms of blueberries to nitrogen deficiency stress

Cited by 5 publications

References 38 publications

Comparative Transcriptome Analysis Reveals the Protective Role of Melatonin during Salt Stress by Regulating the Photosynthesis and Ascorbic Acid Metabolism Pathways in Brassica campestris

Comparative Transcriptome Analysis Reveals the Protective Role of Melatonin during Salt Stress by Regulating the Photosynthesis and Ascorbic Acid Metabolism Pathways in Brassica campestris

Transcriptomic and Metabolomic Profiling Reveals the Variations in Carbohydrate Metabolism between Two Blueberry Cultivars

Identification of crucial metabolites in colored grain wheat (Triticum aestivum L.) regulated by nitrogen application

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