Transcriptomic and genetic approaches reveal an essential role of the NAC transcription factor SlNAP1 in the growth and defense response of tomato

Wang, Jiao; Zheng, Chenfei; Shao, Xiangqi; Hu, Zhangjian; Li, Jianxin; Wang, Ping; Wang, Anran; Yu, Jingquan; Shi, Kai

doi:10.1038/s41438-020-00442-6

Cited by 48 publications

(35 citation statements)

References 44 publications

(57 reference statements)

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“…Simulation result indicated that a 10% reduction in maize yield was shown for each 1 °C increase in global temperature (Zhang and Zhao, 2017;Dong et al, 2021). Furthermore, previous studies have underscored the effects of high temperature on maize growth and development (Obaid et al, 2016;Zhang and Zhao, 2017;Wang et al, 2020b). For example, maize plants exhibited various effects of high temperature at distinct phenological periods (Lizaso et al, 2018).…”

Section: Introductionmentioning

confidence: 94%

Heat Stress After Pollination Reduces Kernel Number in Maize by Insufficient Assimilates

Niu

Wei

et al. 2021

Front. Genet.

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Global warming has increased the occurrence of high temperature stress in plants, including maize, resulting in decreased the grain number and yield. Previous studies indicate that heat stress mainly damages the pollen grains and thus lowered maize grain number. Other field studies have shown that heat stress after pollination results in kernel abortion. However, the mechanism by which high temperature affect grain abortion following pollination remains unclear. Hence, this study investigated the field grown heat-resistant maize variety “Zhengdan 958” (ZD958) and heat-sensitive variety “Xianyu 335” (XY335) under a seven-day heat stress treatment (HT) after pollination. Under HT, the grain numbers of XY335 and ZD958 were reduced by 10.9% (p = 0.006) and 5.3% (p = 0.129), respectively. The RNA sequencing analysis showed a higher number of differentially expressed genes (DEGs) between HT and the control in XY335 compared to ZD958. Ribulose diphosphate carboxylase (RuBPCase) genes were downregulated by heat stress, and RuBPCase activity was significantly lowered by 14.1% (p = 0.020) in XY335 and 5.3% (p = 0.436) in ZD958 in comparison to CK. The soluble sugar and starch contents in the grains of XY335 were obviously reduced by 26.1 and 58.5%, respectively, with no distinct change observed in ZD958. Heat stress also inhibited the synthesis of grain starch, as shown by the low activities of metabolism-related enzymes. Under HT, the expression of trehalose metabolism genes in XY335 were upregulated, and these genes may be involved in kernel abortion at high temperature. In conclusion, this study revealed that post-pollination heat stress in maize mainly resulted in reduced carbohydrate availability for grain development, though the heat-resistant ZD958 was nevertheless able to maintain growth.

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Section: Introductionmentioning

confidence: 94%

Heat Stress After Pollination Reduces Kernel Number in Maize by Insufficient Assimilates

Niu

Wei

et al. 2021

Front. Genet.

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“…In addition, the roles of cell wall proteins in defense against R. solanacearum in tomato have been discussed extensively ( Wydra and Beri, 2006 ; Diogo and Wydra, 2007 ; Dahal et al, 2010 ; Schacht et al, 2011 ). Moreover, several recent studies have identified genes related to plant defense against R. solanacearum , including StMKK1 in potato ( Chen et al, 2021 ), SlNAP1 and a bacterial effector protein RipAK in tomato ( Wang et al, 2020 ; Wang Y. et al, 2021 ), and CaNAC2c in pepper ( Cai et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptome Profiling Reveals Defense-Related Genes Against Ralstonia solanacearum Infection in Tobacco

et al. 2021

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Bacterial wilt (BW) caused by Ralstonia solanacearum (R. solanacearum), is a vascular disease affecting diverse solanaceous crops and causing tremendous damage to crop production. However, our knowledge of the mechanism underlying its resistance or susceptibility is very limited. In this study, we characterized the physiological differences and compared the defense-related transcriptomes of two tobacco varieties, 4411-3 (highly resistant, HR) and K326 (moderately resistant, MR), after R. solanacearum infection at 0, 10, and 17 days after inoculation (dpi). A total of 3967 differentially expressed genes (DEGs) were identified between the HR and MR genotypes under mock condition at three time points, including1395 up-regulated genes in the HR genotype and 2640 up-regulated genes in the MR genotype. Also, 6,233 and 21,541 DEGs were induced in the HR and MR genotypes after R. solanacearum infection, respectively. Furthermore, GO and KEGG analyses revealed that DEGs in the HR genotype were related to the cell wall, starch and sucrose metabolism, glutathione metabolism, ABC transporters, endocytosis, glycerolipid metabolism, and glycerophospholipid metabolism. The defense-related genes generally showed genotype-specific regulation and expression differences after R. solanacearum infection. In addition, genes related to auxin and ABA were dramatically up-regulated in the HR genotype. The contents of auxin and ABA in the MR genotype were significantly higher than those in the HR genotype after R. solanacearum infection, providing insight into the defense mechanisms of tobacco. Altogether, these results clarify the physiological and transcriptional regulation of R. solanacearum resistance infection in tobacco, and improve our understanding of the molecular mechanism underlying the plant-pathogen interaction.

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“…Total RNA was extracted from the second leaf (from top to down) of a seven-leaf seedling, following the method described previously ( Wang et al, 2020 ). Total RNA (1 μg) was reverse-transcribed into cDNA as described before ( Hu et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

BRASSINAZOLE RESISTANT 1 Mediates Brassinosteroid-Induced Calvin Cycle to Promote Photosynthesis in Tomato

Tang

Xia

et al. 2022

Front. Plant Sci.

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Calvin cycle is a sequence of enzymatic reactions that assimilate atmospheric CO2 in photosynthesis. Multiple components are known to participate in the induction or suppression of the Calvin cycle but the mechanism of its regulation by phytohormones is still unclear. Brassinosteroids (BRs) are steroid phytohormones that promote photosynthesis and crop yields. In this study, we study the role of BRs in regulating Calvin cycle genes to further understand the regulation of the Calvin cycle by phytohormones in tomatoes. BRs and their signal effector BRASSINAZOLE RESISTANT 1 (BZR1) can enhance the Calvin cycle activity and improve the photosynthetic ability. BRs increased the accumulation of dephosphorylated form of BZR1 by 94% and induced an 88–126% increase in the transcription of key genes in Calvin cycle FBA1, RCA1, FBP5, and PGK1. BZR1 activated the transcription of these Calvin cycle genes by directly binding to their promoters. Moreover, silencing these Calvin cycle genes impaired 24-epibrassinolide (EBR)-induced enhancement of photosynthetic rate, the quantum efficiency of PSII, and Vc,max and Jmax. Taken together, these results strongly suggest that BRs regulate the Calvin cycle in a BZR1-dependent manner in tomatoes. BRs that mediate coordinated regulation of photosynthetic genes are potential targets for increasing crop yields.

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Transcriptomic and genetic approaches reveal an essential role of the NAC transcription factor SlNAP1 in the growth and defense response of tomato

Cited by 48 publications

References 44 publications

Heat Stress After Pollination Reduces Kernel Number in Maize by Insufficient Assimilates

Heat Stress After Pollination Reduces Kernel Number in Maize by Insufficient Assimilates

Comparative Transcriptome Profiling Reveals Defense-Related Genes Against Ralstonia solanacearum Infection in Tobacco

BRASSINAZOLE RESISTANT 1 Mediates Brassinosteroid-Induced Calvin Cycle to Promote Photosynthesis in Tomato

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