Transcriptomic and biochemical analyses of drought response mechanism in mung bean (Vignaradiata (L.) Wilczek) leaves

Abstract

Drought is a major factor that limiting mung bean development. To clarify the molecular mechanism of mung bean in response to drought stress, 2 mung bean groups were established, the experimental group (drought-treated) and the control group (normal water management). With prominent difference of 2 groups in stomatal conductance, relative water content and phenotype, leaf samples were collected at 4 stages, and the physiological index of MDA, POD, chlorophyll, and soluble proteins were estimated. RNA-seq was used to obtain high quality data of samples, and differentially expressed genes were identified by DESeq2. With GO and KEGG analysis, DEGs were enriched into different classifications and pathways. WGCNA was used to detect the relationship between physiological traits and genes, and qPCR was performed to confirm the accuracy of the data. We obtained 169.49 Gb of clean data from 24 samples, and the Q30 of each date all exceeded 94%. In total, 8963 DEGs were identified at 4 stages between the control and treated samples, and the DEGs were involved in most biological processes. 1270 TFs screened from DEGs were clustered into 158 TF families, such as AP2, RLK-Pelle-DLSVA, and NAC TF families. Genes related to physiological traits were closely related to plant hormone signaling, carotenoid biosynthesis, chlorophyll metabolism, and protein processing. This paper provides a large amount of data for drought research in mung bean.

Genome-wide identification, molecular evolution and expression analysis of the B-box gene family in mung bean (Vigna radiata L.)

“…The PLACE website ( http://www.dna.affrc.go.jp/PLACE/signalscan.html ) [ 62 ] was used to identify the cis -acting elements involved in abiotic stress and hormone responses. To gain insight into the expression patterns of VrBBXs genes under drought stress, RNA-Seq data (BioProject: PRJNA764584) was obtained from SRA-NCBI ( https://www.ncbi.nlm.nih.gov/sra ) [ 63 ]. Paired-end clean reads were downloaded and aligned to the reference genome using bowtie2.…”

Progress in the Use of Combined Omics for Mungbean Breeding Improvement and Its Potential in Promoting Resistance against Cercospora Leaf Spot

“…Lim et al [123] informed on transcriptomic changes in mungbean sprouts through RNA sequencing, where 728 DEGs were identified for several metabolite biosynthetic pathways. In the recently conducted study by Guo et al [5] on transcriptomic and biochemical analyses of drought response mechanism in mungbean leaves, RNA sequencing-based DEGs were analysed to clarify the molecular mechanism of mungbean in response to drought stress. In this study, the relationship between molecular and physiological changes was analysed to screen candidate gene responses to drought stress in mungbean, which provides a vital foundation for future studies assessing drought resistance in mungbean.…”

“…Mungbean [Vigna radiata (L.) Wilczek] or greengram is a self-pollinated, fast-growing, and drought-tolerant legume crop having trifoliate leaves, diploid chromosome set, 2n = 2x = 22, with a genome size ranging from 494 to 579 megabases (Mbs) and mostly adapted to all the tropics and subtropic areas of the world [1][2][3][4][5][6]. Mungbean is the most important legume crop for the agricultural production system and a great source of high-quality protein, minerals, and vitamins [7,8].…”

Physiological responses to drought stress of three pine species and comparative transcriptome analysis of Pinus yunnanensis var. pygmaea

“…Osmoprotectants such as total soluble sugars and proteins were early accumulated in primed plants during the stress [ 22 ]. Meanwhile, antioxidant protection systems perform an essential function during drought stress [ 23 ]. Many cell components are damaged by reactive oxygen species (ROS) that are generated as a result of decreases in photosynthesis [ 24 ].…”