Transcriptomic analysis supports collective endometrial cell migration in the pathogenesis of adenomyosis

Zhai, Junyu; Li, Shang; Sen, Sushmita; Vallvé-Juanico, Júlia; Irwin, Juan C.; Vo, Kim Chi; Wan, Jipeng; Du, Yanzhi; Chen, Zi‐Jiang; Giudice, Linda C.

doi:10.1016/j.rbmo.2022.05.007

Cited by 11 publications

(6 citation statements)

References 49 publications

(62 reference statements)

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“…Another recent study compared endometrial and myometrial tissues of women with or without diffuse adenomyosis at the transcriptomic level ( 54 ). These investigators found that biological processes, including inflammation, extracellular matrix organization, collagen degradation, hyaluronan synthesis, and cell migration, were upregulated in adenomyosis.…”

Section: Discussionmentioning

confidence: 99%

Adenomyosis: single-cell transcriptomic analysis reveals a paracrine mesenchymal–epithelial interaction involving the WNT/SFRP pathway

Yıldız¹,

Kinali²,

Wei³

et al. 2023

Fertility and Sterility

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Section: Discussionmentioning

confidence: 99%

Adenomyosis: single-cell transcriptomic analysis reveals a paracrine mesenchymal–epithelial interaction involving the WNT/SFRP pathway

Yıldız¹,

Kinali²,

Wei³

et al. 2023

Fertility and Sterility

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“…Among the genes upregulated in F. vaginae infected organoids in comparison to the negative controls and to the L. crispatus infected organoids, multiple genes encoding C-X-C motif chemokine ligand (CXCL) family were represented. The CXCL family widely participates in immune cell recruitment, specifically, attracts monocytes and neutrophils, and is involved in the pathogenesis of inflammatory diseases such as endometriosis and its associated infertility [27][28][29] as well as angiogenesis and tumor progression in ovarian and cervical cancer 30,31 . C5 that is highly upregulated in the F. vaginae infected organoids is a chemotactic molecule that directly attracts neutrophils, eosinophils, basophils, and monocytes to the sites where complement is activating 32 .…”

Section: Discussionmentioning

confidence: 99%

Vaginal bacteria elicit acute inflammatory response in fallopian tube organoids: a model for pelvic inflammatory disease

2023

Preprint

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Objective: To facilitate in vitro mechanistic studies in pelvic inflammatory disease (PID) and subsequent tubal factor infertility, as well as ovarian carcinogenesis, we sought to establish patient tissue derived fallopian tube (FT) organoids and to study their inflammatory response to acute vaginal bacterial infection. Design: Experimental study. Setting: Academic medical and research center. Patients: FT tissues were obtained from four patients after salpingectomy for benign gynecological diseases. Interventions: We introduced acute infection in the FT organoid culture system by inoculating the organoid culture media with two common vaginal bacterial species, Lactobacillus crispatus and Fannyhessea vaginae. Main Outcome Measures: The inflammatory response elicited in the organoids after acute bacterial infection was analyzed by the expression profile of 249 inflammatory genes. Results: Compared to the negative controls that were not cultured with any bacteria, the organoids cultured with either bacterial species showed multiple differentially expressed inflammatory genes. Marked differences were noted between the Lactobacillus crispatus infected organoids and those infected by Fannyhessea vaginae. Genes from the C-X-C motif chemokine ligand (CXCL) family were highly upregulated in F. vaginae infected organoids. Flow cytometry showed that immune cells quickly disappeared during the organoid culture, indicating the inflammatory response observed with bacterial culture was generated by the epithelial cells in the organoids. Conclusion: Patient tissue derived FT organoids respond to acute bacterial infection with upregulation of inflammatory genes specific to different vaginal bacterial species. FT organoids is a useful model system to study the host-pathogen interaction during bacterial infection which may facilitate mechanistic investigations in PID and its contribution to tubal factor infertility and ovarian carcinogensis.

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“…42 A recent transcriptome-based study highlighted that CCM is the main mechanism for AM eutopic endometrium invasiveness. 43…”

Section: Aams and Invasion/migrationmentioning

confidence: 99%

“…Collective cell migration (CCM) involves the formation of an invasive cell population that can advance, attach, and rearrange the surrounding extracellular matrix (ECM), and plays a crucial role in processes, including wound healing, tissue regeneration, and cancer metastasis 42 . A recent transcriptome‐based study highlighted that CCM is the main mechanism for AM eutopic endometrium invasiveness 43 . Subsequently, Stratopoulou et al.…”

Section: The Role and Regulatory Mechanism Of Aamsmentioning

confidence: 99%

Macrophage polarization in adenomyosis: A review

Qiu,

Cao,

et al. 2024

American J Rep Immunol

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Adenomyosis (AM) is a common gynecological disorder characterized by the presence of endometrial glands and stroma within the uterine myometrium. It is associated with abnormal uterine bleeding (AUB), dysmenorrhea, and infertility. Although several mechanisms have been proposed to elucidate AM, the exact cause and development of the condition remain unclear. Recent studies have highlighted the significance of macrophage polarization in the microenvironment, which plays a crucial role in AM initiation and progression. However, a comprehensive review regarding the role and regulatory mechanism of macrophage polarization in AM is currently lacking. Therefore, this review aims to summarize the phenotype and function of macrophage polarization and the phenomenon of the polarization of adenomyosis‐associated macrophages (AAMs). It also elaborates on the role and regulatory mechanism of AAM polarization in invasion/migration, fibrosis, angiogenesis, dysmenorrhea, and infertility. Furthermore, this review explores the underlying molecular mechanisms of AAM polarization and suggests future research directions. In conclusion, this review provides a new perspective on understanding the pathogenesis of AM and provides a theoretical foundation for developing targeted drugs through the regulation of AAM polarization.

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Transcriptomic analysis supports collective endometrial cell migration in the pathogenesis of adenomyosis

Cited by 11 publications

References 49 publications

Adenomyosis: single-cell transcriptomic analysis reveals a paracrine mesenchymal–epithelial interaction involving the WNT/SFRP pathway

Adenomyosis: single-cell transcriptomic analysis reveals a paracrine mesenchymal–epithelial interaction involving the WNT/SFRP pathway

Vaginal bacteria elicit acute inflammatory response in fallopian tube organoids: a model for pelvic inflammatory disease

Macrophage polarization in adenomyosis: A review

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