Transcriptomic analysis reveals the role of FOUR LIPS in response to salt stress in rice

Zhang, Chunxia; Zhang, Jie; Liu, Huichao; Qu, Xiaoxiao; Wang, Junxue; He, Qixiumei; Zou, Junjie; Yang, Kezhen; Le, Jie

doi:10.1007/s11103-022-01282-9

Cited by 6 publications

(3 citation statements)

References 65 publications

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“…Activation of antioxidant systems such as peroxidases (PRXs), catalases (CATs), and superoxide dismutases (SODs) facilitate ROS removal and counteract potential molecular damage ( Dietz et al., 2016 ; Steinbrecher and Leubner-Metzger, 2017 ; Noctor et al., 2018 ; Kurek et al., 2019 ; Marks et al., 2022 ). Several peroxidases (PRXs) and Cytochrome P450 genes appeared in our GO analysis results ( Supplementary Table 1 ) and these are generally known to be involved in H 2 O 2 metabolism and enhancing the activity of compounds with increased antioxidant activity ( Almagro et al., 2009 ; Pandian et al., 2020 ; Rao et al., 2020 ; Zhang et al., 2022 ). Other upregulated categories in the GO analysis included apoplast, chloroplast, extracellular region, heme binding, plastid, response to hormone, and response to chemical ( Figure 5 ).…”

Section: Resultsmentioning

confidence: 96%

Growth and transcriptional response of wheat and rice to the tertiary amine BMVE

Dharni,

Shi,

Zhang

et al. 2024

Front. Plant Sci.

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IntroductionSeed vigor is largely a product of sound seed development, maturation processes, genetics, and storage conditions. It is a crucial factor impacting plant growth and crop yield and is negatively affected by unfavorable environmental conditions, which can include drought and heat as well as cold wet conditions. The latter leads to slow germination and increased seedling susceptibility to pathogens. Prior research has shown that a class of plant growth regulators called substituted tertiary amines (STAs) can enhance seed germination, seedling growth, and crop productivity. However, inconsistent benefits have limited STA adoption on a commercial scaleMethodsWe developed a novel seed treatment protocol to evaluate the efficacy of 2-(N-methyl benzyl aminoethyl)-3-methyl butanoate (BMVE), which has shown promise as a crop seed treatment in field trials. Transcriptomic analysis of rice seedlings 24 h after BMVE treatment was done to identify the molecular basis for the improved seedling growth. The impact of BMVE on seed development was also evaluated by spraying rice panicles shortly after flower fertilization and subsequently monitoring the impact on seed traits.ResultsBMVE treatment of seeds 24 h after imbibition consistently improved wheat and rice seedling shoot and root growth in lab conditions. Treated wheat seedlings grown to maturity in a greenhouse also resulted in higher biomass than controls, though only under drought conditions. Treated seedlings had increased levels of transcripts involved in reactive oxygen species scavenging and auxin and gibberellic acid signaling. Conversely, several genes associated with increased reactive oxygen species/ROS load, abiotic stress responses, and germination hindering processes were reduced. BMVE spray increased both fresh and mature seed weights relative to the control for plants exposed to 96 h of heat stress. BMVE treatment during seed development also benefited germination and seedling growth in the next generation, under both ambient and heat stress conditions.DiscussionThe optimized experimental conditions we developed provide convincing evidence that BMVE does indeed have efficacy in plant growth enhancement. The results advance our understanding of how STAs work at the molecular level and provide insights for their practical application to improve crop growth.

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Section: Resultsmentioning

confidence: 96%

Growth and transcriptional response of wheat and rice to the tertiary amine BMVE

Dharni,

Shi,

Zhang

et al. 2024

Front. Plant Sci.

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“…In addition to EIL , the yeast one hybrid analysis identified seven other families of transcription factor families that can bind the SiR promoter. Many play documented roles during abiotic or biotic stress, with for example OBF1, ERF3, NAP and FLP acting during low-temperature or drought responses (Shimizu et al 2005; Zhang et al 2013, 2022; Chen et al 2014; Qu et al 2022), while TCP21, EREBP1, ERF3, ERF72, ERF83 are involved in both abiotic and biotic stress (Lin et al 2007; Jisha et al 2015; Zhang et al 2016, 2018; Tezuka et al 2019; Jung et al 2021). Consistent with previous in silico analysis (Kurt et al 2022) the presence of multiple AP2/ERF and EIL transcription factors binding sites suggests that SiR is likely subject to control from ethylene signalling (Binder 2020) and also of transcription factors that respond to ABA and jasmonic acid biosynthesis and signalling (Yaish et al 2010; Chen et al 2014; Jisha et al 2015; Zhang et al 2016, 2018).…”

Section: Discussionmentioning

confidence: 99%

A transcription factor quintet orchestrating bundle sheath expression in rice

Hua,

Wang,

Stanley

et al. 2024

Preprint

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The C4pathway has evolved in over sixty lineages of plants and improves photosynthetic efficiency by ∼50%. One unifying character of C4plants is photosynthetic activation of a compartment such as the bundle sheath, but gene regulatory networks controlling this cell type are poorly understood. For example, inArabidopsis thalianaa bipartite MYC-MYB transcription factor module restricts gene expression to this cell type but in grasses the regulatory logic allowing bundle sheath gene expression has not been defined. Using the global staple and C3crop rice we identified theSULFITE REDUCTASEpromoter as sufficient for strong expression in this cell type. This promoter encodes an intricatecis-regulatory logic with multiple activators and repressors acting in a combinatorial manner. However, within this landscape we identified a distal enhancer comprising a module activated by quartet of transcription factors from the G2-like, MYB-related, IDD and bZIP families. This module is necessary and sufficient to pattern gene expression to the rice bundle sheath, and oligomerisation allowed activity to be increased 23-fold. In summary, we identify a short tunable enhancer patterning expression to the rice bundle sheath that we anticipate will be useful for engineering this cell type. The transcription factor module responsible for this patterning is distinct from that previously reported inA. thaliana.

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“…At the same time, the expression of MdMYB88 and MdMYB124 are repressed by ABA (Geng et al, 2020; Xie et al, 2021). Overexpressing OsFLP significantly enhances drought and salt tolerance, whereas reducing OsFLP expression levels not only lead to abnormal GMC division and box‐shaped stomata, but also lead to increased sensitivity to drought and salt stress in rice (Qu et al, 2022; Zhang et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

An R2R3 MYB transcription factor PsFLP regulates the symmetric division of guard mother cells during stomatal development in Pisum sativum

Ning

Yang

Chen

et al. 2023

Physiologia Plantarum

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MYB transcriptional regulators belong to one of the most significant transcription factors families in plants, among which R2R3‐MYB transcription factors are involved in plant growth and development, hormone signal transduction, and stress response. Two R2R3‐MYB transcription factors, FLP and its paralogous AtMYB88, redundantly regulate the symmetrical division of guard mother cells (GMCs), and abiotic stress response in Arabidopsis thaliana. Only one orthologue gene of FLP was identified in pea (Pisum sativum FLP; PsFLP). In this study, we explored the gene function of PsFLP by virus‐induced gene silencing (VIGS) technology. The phenotypic analysis displayed that the silencing of PsFLP expression led to the abnormal development of stomata and the emergence of multiple guard cells tightly united. In addition, the abnormal stomata of flp could be fully rescued by PsFLP driven by the FLP promoter. In conclusion, the results showed that PsFLP plays a conservative negative role in regulating the symmetric division of GMC during stomatal development. Based on real‐time quantitative PCR, the relative expressions of AAO3, NCED3, and SnRK2.3 significantly increased in the flp pFLP::PsFLP plants compared to mutant, indicating that PsFLP might be involved in drought stress response. Thus, PsFLP regulates the genes related to cell cycle division during the stomatal development of peas and participates in response to drought stress. The study provides a basis for further research on its function and application in leguminous crop breeding.

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Transcriptomic analysis reveals the role of FOUR LIPS in response to salt stress in rice

Cited by 6 publications

References 65 publications

Growth and transcriptional response of wheat and rice to the tertiary amine BMVE

Growth and transcriptional response of wheat and rice to the tertiary amine BMVE

A transcription factor quintet orchestrating bundle sheath expression in rice

An R2R3 MYB transcription factor PsFLP regulates the symmetric division of guard mother cells during stomatal development in Pisum sativum

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