Transcriptomic analysis reveals the molecular mechanisms of Camellia sinensis in response to salt stress

Integrated Analysis of Long Non-coding RNAs (lncRNAs) and mRNAs Reveals the Regulatory Role of lncRNAs Associated With Salt Resistance in Camellia sinensis

“…In addition, the cis-targeting and trans-targeting relationship between lncRNAs and mRNAs and the possible interactions between lncRNAs and abiotic stress-related miRNAs were found to be involved in salt stress in tea plants. Notably, in our previous report, the Ca 2+ -transporting ATPase was shown to be involved in the salt-stress response in tea plants (Wan et al, 2018), and in this study, the differentially expressed Ca 2+ -transporting ATPase 13 (TEA027212.1) was proven to be co-expressed with the lncRNA MSTRG.139242.1, which indicates that this lncRNA may participate in Ca 2+ signal transduction in response to salt stress. In addition, six DE-lncRNAs were randomly selected for quantitative real-time PCR (qRT-PCR) validation to confirm the reliability of the expression levels obtained from the RNA-Seq transcriptome.…”

“…In our previous report, we sequenced six tea plant samples using RNA-Seq technology. A total of 79.40 Gb of clean data was obtained, and the Q30 base percentage of each sample was greater than 92.93% (Wan et al, 2018). The identification of lncRNAs was executed according to the pipeline shown in Figure 1A.…”

“…First-strand cDNA was synthesized using the PrimeScript RT reagent kit with gDNA Eraser (TaKaRa, Dalian, China), after which qRT-PCR detection was completed using the EvaGreen qPCR MasterMix-No Dye kit (ABM, Richmond, BC, Canada) on a StepOne Plus PCR instrument (ABI, United States) following the manufacturers' protocols. The tea plant Csβ-actin gene was chosen as the reference gene in accordance with previous methods (Wan et al, 2018), and the primers used in this assay are shown in Supplementary Data sheet S1: Table S2. The relative expression levels of the genes were calculated using the 2 − CT method (Livak and Schmittgen, 2001), and the data are presented as the mean ± SD from three independent biological replicates.…”

“…To identify salt-responsive RNAs in C. sinensis, six cDNA libraries from three controls (Control1, Control2, Control3) and three salt-treated (Salt1, Salt2, Salt3) leaf samples that were treated with 250 mM NaCl for 4 h were constructed and sequenced using an Illumina HiSeq2500 platform in our previous report (Wan et al, 2018). In this report, the RNA-Seq results were aligned to the latest tea tree genome, and the alignment results are shown in Table 1.…”

“…Salt stress is a form of abiotic stress that severely affects the growth of tea plants and the quality of tea products (Upadhyaya and Panda, 2013). In our previous report, we performed an Illumina RNA sequencing (RNA-Seq) to compare the transcriptomes of tea plants treated with and without NaCl, and many differentially expressed mRNAs involved in signal transduction pathways, transcription factors (TFs), and other functional genes under salt stress were identified (Wan et al, 2018). Previous reports have also shown that many mRNAs and proteins in tea plants, such as CsSnRK2 (Zhang et al, 2018), CsAQP (Yue et al, 2014), and CsVQ (Guo et al, 2018), respond to salt stress.…”