Transcriptomic analysis reveals putative osmoregulation mechanisms in the kidney of euryhaline turbot Scophthalmus maximus responded to hypo-saline seawater

Cui, Wenxiao; Ma, Aijun; Huang, Zhihui; Wang, Xinan; Sun, Zhibin; Liu, Zhifeng; Wei, Zhang; Yang, Jingkun; Zhang, Jinsheng; Qu, Junle

doi:10.1007/s00343-019-9056-2

Cited by 16 publications

(16 citation statements)

References 47 publications

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“…The PI3K-AKT signalling pathway, which is a crucial part of signal transduction network, has been found to be involved in many important physiological processes and stress responses to environmental fluctuations in parameters such as salinity (Cui et al, 2019;Yan et al, 2018;Yu & Cui, 2016). The results of the present study demonstrated for the first time that the PI3K-AKT signalling pathway is involved in osmoregulation in turbot.…”

Section: Discussionsupporting

confidence: 57%

“…Principles and patterns of osmoregulation have been identified in many euryhaline fish (Edwards & Marshall, 2012; Kültz, Chakravarty, & Adilakshmi, 2001), but the signal transduction network composed of signalling pathways is poorly understood. The PI3K‐AKT signalling pathway, which is a crucial part of signal transduction network, has been found to be involved in many important physiological processes and stress responses to environmental fluctuations in parameters such as salinity (Cui et al, 2019; Yan et al, 2018; Yu & Cui, 2016). The results of the present study demonstrated for the first time that the PI3K‐AKT signalling pathway is involved in osmoregulation in turbot.…”

Section: Discussionmentioning

confidence: 99%

“…The gene expression profiles of transcriptome data (SRP153594, PRJNA596587) were generated previously using an Illumina Hiseq 2000 platform (Cui et al, 2019). The transcriptome data set included three hypo‐salinity (5‰)‐ and three seawater (30‰)‐treated kidney and gill samples of turbot (total length 10.14 ± 0.29 cm) exposed to treatments for 24 hr respectively.…”

Section: Methodsmentioning

confidence: 99%

“…The specificity and efficiency of all primers for qRT‐PCR were validated by sequencing and then blast to NCBI and by running serial dilutions and creating a calibration curve respectively. The housekeeping gene β ‐actin was used to adjust the gene expression data in order to keep the correct rate of pre‐analysis gene expression data (Cui et al, 2019). qRT‐PCR was performed following the protocol provided by the TransStart Top Green qPCR SuperMix Kit (Beijing TransGen Biotech Co. Ltd.) on a 185–5096 CFX96 Real‐Time PCR Detection System (Beijing yue da Biotechnology Co. Ltd.).…”

Section: Methodsmentioning

confidence: 99%

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Response of the PI3K‐AKT signalling pathway to low salinity and the effect of its inhibition mediated by wortmannin on ion channels in turbot Scophthalmus maximus

Cui

Wang

2020

Aquac Res

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The PI3K-AKT signalling pathway is a crucial part of the signal transduction network and is involved in many important physiological processes and the stress response to environmental alteration. In this study, gill and kidney transcriptome data demonstrated that the PI3K-AKT signalling pathway plays an important role in the stress response of the turbot Scophthalmus maximus when exposed to low salinity.Low-salinity stress led to down-regulation of gene expression, protein content and phosphorylation of AKT1 at Thr308 of the PI3K-AKT signalling pathway. However, the abundance and phosphorylation of AKT1 protein at Ser473 and Thr308 were detected only in the gill and not in the kidney. Inhibition of the PI3K-AKT signalling pathway mediated by wortmannin showed that wortmannin remarkably inhibited PI3K gene expression and protein synthesis as well as phosphorylation of AKT1 at Thr308 with or without low-salinity stress. In the context of pathway inhibition, suppressed expression levels of ion channel genes demonstrated that the PI3K-AKT signalling pathway acts on osmoregulation by positively regulating ion transport. K E Y W O R D S ion channels, low salinity, PI3K-AKT signalling pathway, turbot Scophthalmus maximus, wortmannin | 2677 CUI et al.

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Section: Discussionsupporting

confidence: 57%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Response of the PI3K‐AKT signalling pathway to low salinity and the effect of its inhibition mediated by wortmannin on ion channels in turbot Scophthalmus maximus

Cui

Wang

2020

Aquac Res

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“…In recent years, numerous RNA-seq studies have been conducted to explore the stress responsive genes and molecular mechanisms under various stresses, such as pathogens stress ( Enteromyxum scophthalmi 3 , 4 , Vibrio anguillarum 5 ), heat stress 6 , oxygen stress 7 , crowding stress 8 , salinity stress 9 , and feeding stress 10 . All these researches were solely focused on the identification of differentially expressed genes (DEGs), whereas connectivity analysis has not yet been taken into account.…”

Section: Background and Summarymentioning

confidence: 99%

Identification of stress-related genes by co-expression network analysis based on the improved turbot genome

Zheng

Meng

et al. 2022

Sci Data

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Turbot (Scophthalmus maximus), commercially important flatfish species, is widely cultivated in Europe and China. With the continuous expansion of the intensive breeding scale, turbot is exposed to various stresses, which greatly impedes the healthy development of turbot industry. Here, we present an improved high-quality chromosome-scale genome assembly of turbot using a combination of PacBio long-read and Illumina short-read sequencing technologies. The genome assembly spans 538.22 Mb comprising 27 contigs with a contig N50 size of 25.76 Mb. Annotation of the genome assembly identified 104.45 Mb repetitive sequences, 22,442 protein-coding genes and 3,345 ncRNAs. Moreover, a total of 345 stress responsive candidate genes were identified by gene co-expression network analysis based on 14 published stress-related RNA-seq datasets consisting of 165 samples. Significantly improved genome assembly and stress-related candidate gene pool will provide valuable resources for further research on turbot functional genome and stress response mechanism, as well as theoretical support for the development of molecular breeding technology for resistant turbot varieties.

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Transcriptome Analysis Revealed Osmoregulation Related Regulatory Networks and Hub Genes in the Gills of Hilsa shad, Tenualosa ilisha, during the Migratory Osmotic Stress

et al. 2023

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Transcriptomic analysis reveals putative osmoregulation mechanisms in the kidney of euryhaline turbot Scophthalmus maximus responded to hypo-saline seawater

Cited by 16 publications

References 47 publications

Response of the PI3K‐AKT signalling pathway to low salinity and the effect of its inhibition mediated by wortmannin on ion channels in turbot Scophthalmus maximus

Response of the PI3K‐AKT signalling pathway to low salinity and the effect of its inhibition mediated by wortmannin on ion channels in turbot Scophthalmus maximus

Identification of stress-related genes by co-expression network analysis based on the improved turbot genome

Transcriptome Analysis Revealed Osmoregulation Related Regulatory Networks and Hub Genes in the Gills of Hilsa shad, Tenualosa ilisha, during the Migratory Osmotic Stress

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