Transcriptomic Analysis Reveals Possible Influences of ABA on Secondary Metabolism of Pigments, Flavonoids and Antioxidants in Tomato Fruit during Ripening

Mou, Wangshu; Li, Dongdong; Luo, Zisheng; Mao, Linchun; Ying, Tiejin

doi:10.1371/journal.pone.0129598

Cited by 77 publications

(56 citation statements)

References 125 publications

(165 reference statements)

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“…Moreover, the transcription factor MYB could be activated by ABA, then bound to bHLH and WD40 to form a protein complex, which increased anthocyanin biosynthesis [31]. In addition, FLS and UDPG in the flavonoid biosynthesis pathway were higher expressed by ABA as well [18]. Our results from the present study further confirmed the positive role of ABA in fruit flavonoid biosynthesis ( Figure 5).…”

Section: Discussionsupporting

confidence: 82%

“…In grape berries, it was reported that pre-harvest exogenous MLT treatment significantly increased the polyphenolic content, antioxidant capacity, and related gene expressions, and improved the fruit maturity [12].Abscisic acid (ABA) is one of the crucial plant hormones, which play vital roles in fruit ripening and development. Studies reported that exogenous ABA promoted fruit coloration, including anthocyanin and flavanol accumulation during fruit ripening in apple [13], citrus [14], grape [15], litchi [16], strawberry [17], and tomato [18]. It was also reported that the transcriptional levels of phenylalanine ammonia-lyase (PAL), cinnamate-4-hydroxylase (C4H), chalcone synthase (CHS), chalcone isomerase (CHI), dihydroflavonol 4-reductase (DFR), leucoanthocyanidin dioxygenase (LDOX) genes involved in polyphenolic biosynthesis were significantly increased by exogenous ABA treatment [16].…”

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confidence: 99%

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Exogenous Melatonin and Abscisic Acid Expedite the Flavonoids Biosynthesis in Grape Berry of Vitis vinifera cv. Kyoho

et al. 2019

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Grape polyphenols contributing to more than half of the global polyphenol market were well studied; however, how melatonin (MLT), a potential plant hormone, and abscisic acid (ABA) affects polyphenols profile is still poorly understood. To explore whether these hormones are involved in polyphenolic biosynthesis, grape (Vitis vinifera cv. Kyoho) was exposed to MLT, ABA, and NDGA (nordihydroguaiaretic acid, an ABA biosynthesis inhibitor) treatments, and 16 polyphenols were identified from grape extracts by high performance liquid chromatography quadrupole time of flight mass spectrometry (HPLC-Q-TOF-MS). Both exogenous MLT and ABA significantly enhanced the biosynthesis of each flavonol and flavanol component, especially catechin, which was almost increased double by 200 µM of MLT treatment. Furthermore, the expression of genes involved in flavonoid biosynthesis, including 4-coumaroyl-CoA synthase, chalcone synthase, flavonoid 3′-hydroxylase, anthocyanin 3′-methyltransferase, flavonol synthase, flavonoid-3-O-glucosyltransferase, and flavonoid 3′,5′-methyltransferase were highly up-regulated as well but were down-regulated by NDGA. The present study provided new insights for improving flavonoids accumulation in agricultural production and its underlying mechanism.

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Section: Discussionsupporting

confidence: 82%

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confidence: 99%

Exogenous Melatonin and Abscisic Acid Expedite the Flavonoids Biosynthesis in Grape Berry of Vitis vinifera cv. Kyoho

et al. 2019

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“…(ii) Melatonin may affect TFs to control fruit ripening directly (ethylene-independent means). Additionally, current evidence supports the notion that ABA is responsible for controlling fruit ripening [57]. Exogenous ABA was found to enhance the transcription of genes involved in pigments metabolism.…”

Section: Discussionsupporting

confidence: 81%

A label‐free differential proteomics analysis reveals the effect of melatonin on promoting fruit ripening and anthocyanin accumulation upon postharvest in tomato

Sun

Zhang

Wang

et al. 2016

Journal of Pineal Research

166

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To better understand the function of melatonin in tomato fruit ripening and quality improvement, a label-free quantitation method was used to investigate the proteins that differ between the control (CK) and 50 μm melatonin treatment (M50) fruits. Proteomics data identified 241 proteins that were significantly influenced by melatonin. These proteins were involved in several ripening-related pathways, including cell wall metabolism, oxidative phosphorylation, carbohydrate, and fatty acid metabolism. Moreover, the application of exogenous melatonin increased eight proteins that are related to anthocyanin accumulation during fruit ripening. Additionally, the affected protein levels correlated with the corresponding gene transcript levels. Further, the total anthocyanin content from M50 increased by 52%, 48%, and 50% at 5, 8, and 13 DAT (day after melatonin treatment), respectively. The melatonin-mediated promotion of fruit ripening and quality might be due to the altered proteins involved in processes associated with ripening. In this work, we indicated that a senescence-related protein was downregulated in the M50 fruit, while a cell apoptosis inhibitor (API5) protein was upregulated. In addition, peroxidases (POD9, POD12, peroxidase p7-like) and catalase (CAT3) significantly increased in the M50 fruits. Based on the previous studies and our data, we inferred that melatonin might be positively related to fruit ripening but negatively related to fruit senescence. This research provides insights into the physiological and molecular mechanisms underlying melatonin-mediated fruit ripening as well as the anthocyanin formation process in tomato fruit at the protein concentration level, and we reveal possible candidates for regulation of anthocyanin formation during fruit ripening.

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“…The extent of changes (>10-fold for some flavonoid glycosides) suggests they represent direct effects of the genetic manipulation and thus of the increase in ABA in LCYb-overexpressing fruits, compared to the less pronounced alterations observed in primary metabolites, which are likely to represent secondary effects. This hypothesis finds support in a series of previous studies linking ABA and flavonoid levels in apple (Lu et al, 2017), soybean (Gupta et al, 2018) and tomato (Mou et al, 2015). In agreement with biochemical data, a series of key structural phenylpropanoid genes was up-regulated in LCYb-overexpressing fruits: for instance, PHENYLALANINE AMMONIA-LYASE (PAL) at the MG and B stages, and CHALCONE SYNTHASE (CHS) at the MG and B+10 stages.…”

Section: Metabolic Alterations In Lcyb-overexpressing Fruitssupporting

confidence: 83%

Manipulation of β‐carotene levels in tomato fruits results in increased ABA content and extended shelf life

Diretto

Frusciante

Fabbri

et al. 2019

Plant Biotechnology Journal

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Summary Tomato fruit ripening is controlled by the hormone ethylene and by a group of transcription factors, acting upstream of ethylene. During ripening, the linear carotene lycopene accumulates at the expense of cyclic carotenoids. Fruit‐specific overexpression of LYCOPENE β‐CYCLASE (LCYb) resulted in increased β‐carotene (provitamin A) content. Unexpectedly, LCYb‐overexpressing fruits also exhibited a diverse array of ripening phenotypes, including delayed softening and extended shelf life. These phenotypes were accompanied, at the biochemical level, by an increase in abscisic acid (ABA) content, decreased ethylene production, increased density of cell wall material containing linear pectins with a low degree of methylation, and a thicker cuticle with a higher content of cutin monomers and triterpenoids. The levels of several primary metabolites and phenylpropanoid compounds were also altered in the transgenic fruits, which could be attributed to delayed fruit ripening and/or to ABA. Network correlation analysis and pharmacological experiments with the ABA biosynthesis inhibitor, abamine, indicated that altered ABA levels were a direct effect of the increased β‐carotene content and were in turn responsible for the extended shelf life phenotype. Thus, manipulation of β‐carotene levels results in an improvement not only of the nutritional value of tomato fruits, but also of their shelf life.

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Transcriptomic Analysis Reveals Possible Influences of ABA on Secondary Metabolism of Pigments, Flavonoids and Antioxidants in Tomato Fruit during Ripening

Cited by 77 publications

References 125 publications

Exogenous Melatonin and Abscisic Acid Expedite the Flavonoids Biosynthesis in Grape Berry of Vitis vinifera cv. Kyoho

Exogenous Melatonin and Abscisic Acid Expedite the Flavonoids Biosynthesis in Grape Berry of Vitis vinifera cv. Kyoho

A label‐free differential proteomics analysis reveals the effect of melatonin on promoting fruit ripening and anthocyanin accumulation upon postharvest in tomato

Manipulation of β‐carotene levels in tomato fruits results in increased ABA content and extended shelf life

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