Transcriptomic analysis reveals peripheral pathway in 3-phenoxybenzoic acid degradation by Aspergillus oryzae M-4

Hu, Kaidi; Li, Jianlong; Zhao, Tianye; Zhou, Qiao; Li, Qin; Hu, Xinjie; Han, Guang; Li, Shuhong; Zou, Likou; Liu, Shuliang

doi:10.1016/j.jenvman.2022.116626

Cited by 3 publications

(1 citation statement)

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“…Transcriptome analysis of A. oryzae M-4 also revealed that 3-PBA induced the upregulation of genes associated with energy expenditure, the oxidative stress response, membrane transport, and DNA repair. In-depth functional interpretation of differentially expressed genes suggested that the involvement of CYP450 may contribute to the hydroxylation of 3-PBA (Hu et al, 2023). Additionally, we observed a fascinating phenomenon: the percentage degradation of 3-PBA in the cellular lysate of the scaengineered bacteria was exceptionally low.…”

Section: Chemical Reagentmentioning

confidence: 78%

Whole-genome sequencing of Sphingobium baderi SC-1 and identification of a crucial 3-phenoxybenzoic acid-degrading gene

Li,

Zhou,

Chen

et al. 2024

Front. Microbiol.

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As an efficient degradation strain, Sphingobium baderi SC-1 can breakdown 3-phenoxybenzoic acid (3-PBA) with high proficiency. To investigate the internal factors that regulate this process, we conducted whole-genome sequencing and successfully identified the pivotal 3-PBA-degrading gene sca (1,230 bp). After sca was expressed in engineered bacteria, a remarkable degradation efficiency was observed, as 20 mg/L 3-PBA was almost completely decomposed within 24 h. The phenol was formed as one of the degradation products. Notably, in addition to their ability to degrade 3-PBA, the resting cells proficiently degraded 4′-HO-3-PBA and 3′-HO-4-PBA. In conclusion, we successfully identified and validated sca as the pivotal enzyme responsible for the efficient degradation of 3-PBA from Sphingomonas baderi, providing a crucial theoretical foundation for further explorations on the degradation potential of SC-1.

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Section: Chemical Reagentmentioning

confidence: 78%