Transcriptomic analysis provides insights on hexavalent chromium induced DNA double strand breaks and their possible repair in midgut cells of Drosophila melanogaster larvae

Mishra, Manish; Sharma, Anurag; Shukla, Arvind Kumar; Pragya, Prakash; Murthy, Ramesh; Pomerai, David de; Dwivedi, Upendra N.; Chowdhuri, D. Kar

doi:10.1016/j.mrfmmm.2013.04.005

Cited by 30 publications

(17 citation statements)

References 69 publications

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“…Hexavalent chromium primarily enters cells leading to ROS formation [Patlolla et al, 2009], and ROS-induced oxidative stress is associated with an increase in DNA single-or double-strand breaks (DSBs) [Klaunig et al, 2010], as observed in midgut cells of exposed Drosophila larvae [Mishra et al, 2013]. Subsequently, unless repaired, DSBs result in DNA lesions and mutagenic effects [Dizdaroglu, 2012].…”

Section: Discussionmentioning

confidence: 99%

Copper oxide nanoparticles and copper sulphate act as antigenotoxic agents in drosophila melanogaster

Alaraby

Hernández

Marcos

2017

Environ and Mol Mutagen

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The biological reactivity of metal and metal oxide nanomaterials is attributed to their redox properties, which would explain their pro- or anti-cancer properties depending on exposure circumstances. In this sense, copper oxide nanoparticles (CuONP) have been proposed as a potential anti-tumoral agent. The aim of this study was to assess if CuONP can exert antigenotoxic effects using Drosophila melanogaster as an in vivo model. Genotoxicity was induced by two well-known genotoxic compounds, namely potassium dichromate (PD) and ethyl methanesulfonate (EMS). The wing-spot assay and the comet assay were used as biomarkers of genotoxic effects. In addition, changes in the expression of Ogg1 and Sod genes were determined. The effects of CuONP cotreatment were compared with those induced by copper sulfate (CS), an agent releasing copper ions. Using the wing-spot assay, CuONP and CS were not able to reduce the genotoxic effects of EMS exposure, but had the ability to decrease the effects induced by PD, reducing the frequency of mutant twin-spots that arise from mitotic recombination. In addition, CuONP and CS were able to reduce the DNA damage induced by PD as determined by the comet assay. In general, similar qualitative antigenotoxic effects were obtained with both copper compounds. The antigenotoxic effects of environmentally relevant and non-toxic doses of CuONP and CS may be explained by their ability to partially restore the expression levels of the repair gene Ogg1 and the antioxidant gene Cu,ZnSod, both of which are inhibited by PD treatment. Environ. Mol. Mutagen. 58:46-55, 2017. © 2016 Wiley Periodicals, Inc.

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Section: Discussionmentioning

confidence: 99%

Copper oxide nanoparticles and copper sulphate act as antigenotoxic agents in drosophila melanogaster

Alaraby

Hernández

Marcos

2017

Environ and Mol Mutagen

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“…Ku80 (genotype: w 1118 , UAS-Ku80 )—Carries an additional copy of gene Ku80 under the control of UAS promoter on chromosome 3. Ku80 is involved in the DSB repair by NHEJ 66 . Ordered from GenetiVision (GenetiVision Houston, USA), with authorship transfer.…”

Section: Methodsmentioning

confidence: 99%

Lifespan and Stress Resistance in Drosophila with Overexpressed DNA Repair Genes

Shaposhnikov

Proshkina

Shilova

et al. 2015

Sci Rep

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DNA repair declines with age and correlates with longevity in many animal species. In this study, we investigated the effects of GAL4-induced overexpression of genes implicated in DNA repair on lifespan and resistance to stress factors in Drosophila melanogaster. Stress factors included hyperthermia, oxidative stress, and starvation. Overexpression was either constitutive or conditional and either ubiquitous or tissue-specific (nervous system). Overexpressed genes included those involved in recognition of DNA damage (homologs of HUS1, CHK2), nucleotide and base excision repair (homologs of XPF, XPC and AP-endonuclease-1), and repair of double-stranded DNA breaks (homologs of BRCA2, XRCC3, KU80 and WRNexo). The overexpression of different DNA repair genes led to both positive and negative effects on lifespan and stress resistance. Effects were dependent on GAL4 driver, stage of induction, sex, and role of the gene in the DNA repair process. While the constitutive/neuron-specific and conditional/ubiquitous overexpression of DNA repair genes negatively impacted lifespan and stress resistance, the constitutive/ubiquitous and conditional/neuron-specific overexpression of Hus1, mnk, mei-9, mus210, and WRNexo had beneficial effects. This study demonstrates for the first time the effects of overexpression of these DNA repair genes on both lifespan and stress resistance in D. melanogaster.

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“…The RNA obtained was reverse transcribed to cDNA using Revert Aid™ H Minus First‐Strand cDNA Synthesis Kit (Fermentas, MD) essentially following the manufacturer's instructions. The expression profiles of selected genes were examined on 7900 HT Fast Real‐Time polymerase chain reaction (PCR) (Applied Biosystems, Germany) according to Mishra et al () with minor modifications. Each reaction was performed in a 96‐well PCR plate format using gene‐specific primers (Table ) and power SYBR Green PCR Master Mix (Applied Biosystems, CA).…”

Section: Methodsmentioning

confidence: 99%

Characterization of the effect of Cr(VI) on humoral innate immunity using Drosophila melanogaster

Pragya

Shukla

Murthy

et al. 2014

Environmental Toxicology

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With the advancement of human race, different anthropogenic activities have heaped the environment with chemicals that can cause alteration in the immune system of exposed organism. As a first line of barrier, the evolutionary conserved innate immunity is crucial for the health of an organism. However, there is paucity of information regarding in vivo assessment of the effect of environmental chemicals on innate immunity. Therefore, we examined the effect of a widely used environmental chemical, Cr(VI), on humoral innate immune response using Drosophila melanogaster. The adverse effect of Cr(VI) on host humoral response was characterized by decreased gene expression of antimicrobial peptides (AMPs) in the exposed organism. Concurrently, a significantly decreased transcription of humoral pathway receptors (Toll and PGRP) and triglyceride level along with inhibition of antioxidant enzyme activities were observed in exposed organism. This in turn weakened the immune response of exposed organism that was manifested by their reduced resistance against bacterial infection. In addition, overexpression of the components of humoral immunity particularly Diptericin benefits Drosophila from Cr(VI)-induced humoral immune-suppressive effect. To our knowledge, this is the first report regarding negative impact of an environmental chemical on humoral innate immune response of Drosophila along with subsequent protection by AMPs, which may provide novel insight into host-chemical interactions. Also, our data validate the utility and sensitivity of Drosophila as a model that could be used for screening the possible risk of environmental chemicals on innate immunity with minimum ethical concern that can be further extrapolated to higher organisms.

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Transcriptomic analysis provides insights on hexavalent chromium induced DNA double strand breaks and their possible repair in midgut cells of Drosophila melanogaster larvae

Cited by 30 publications

References 69 publications

Copper oxide nanoparticles and copper sulphate act as antigenotoxic agents in drosophila melanogaster

Copper oxide nanoparticles and copper sulphate act as antigenotoxic agents in drosophila melanogaster

Lifespan and Stress Resistance in Drosophila with Overexpressed DNA Repair Genes

Characterization of the effect of Cr(VI) on humoral innate immunity using Drosophila melanogaster

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