Transcriptomic analysis of pre-diapause larvae of Chilo suppressalis (Walker) (Lepidoptera: Pyralidae) in natural populations

Dong, Chuan-Lei; Lu, Ming‐Xing; Du, Yu‐Zhou

doi:10.1016/j.cbd.2021.100903

Cited by 4 publications

(3 citation statements)

References 64 publications

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“…TRP channels are also strongly involved in regulating the circadian cycle, consistent with the finding that other genes involved in the modulation of this process were downregulated 24 h after the application of Tenmo-TRP-7 (see also Wolfgang et al 137 ). Our comparative transcriptomic analysis showed that the tested neuropeptide injection at the highest concentration led to a decrease in the expression level of genes encoding regucalcin, takeout-like protein, and circadian clock-controlled protein-like protein 139 – 141 . Recent studies have shown that genes related to the control of circadian clock genes are required to modulate immune system activity, including cellular and humoral responses 142 , 143 .…”

Section: Resultsmentioning

confidence: 87%

Tachykinin-related peptides modulate immune-gene expression in the mealworm beetle Tenebrio molitor L.

Urbański

Johnston

Bittermann

et al. 2022

Sci Rep

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Tachykinins (TKs) are a group of conserved neuropeptides. In insects, tachykinin-related peptides (TRPs) are important modulators of several functions such as nociception and lipid metabolism. Recently, it has become clear that TRPs also play a role in regulating the insect immune system. Here, we report a transcriptomic analysis of changes in the expression levels of immune-related genes in the storage pest Tenebrio molitor after treatment with Tenmo-TRP-7. We tested two concentrations (10–8 and 10–6 M) at two time points, 6 and 24 h post-injection. We found significant changes in the transcript levels of a wide spectrum of immune-related genes. Some changes were observed 6 h after the injection of Tenmo-TRP-7, especially in relation to its putative anti-apoptotic action. Interestingly, 24 h after the injection of 10–8 M Tenmo-TRP-7, most changes were related to the regulation of the cellular response. Applying 10–6 M Tenmo-TRP-7 resulted in the downregulation of genes associated with humoral responses. Injecting Tenmo-TRP-7 did not affect beetle survival but led to a reduction in haemolymph lysozyme-like antibacterial activity, consistent with the transcriptomic data. The results confirmed the immunomodulatory role of TRP and shed new light on the functional homology between TRPs and TKs.

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Section: Resultsmentioning

confidence: 87%

Tachykinin-related peptides modulate immune-gene expression in the mealworm beetle Tenebrio molitor L.

Urbański

Johnston

Bittermann

et al. 2022

Sci Rep

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“…Field-collected larvae were exposed to long photoperiods and high temperatures in the laboratory to terminate diapause, which contrasts with previous studies involving extreme endocrine or pharmacological manipulation. Previous studies mainly focused on comparisons of non-diapausing and diapausing states [ 20 , 24 , 25 , 26 ], or clarifying the threshold of environmental stimuli to induce or terminate diapause [ 16 , 19 , 27 ]. The results of our study provide a roadmap to understand the physiological and molecular mechanisms associated with insect diapause termination, especially in Lepidoptera.…”

Section: Discussionmentioning

confidence: 99%

“…Many studies have focused on revealing the physiological and molecular mechanisms of diapause under field or laboratory conditions. Transcriptomic analysis of field-collected pre-diapausing C. suppressalis larvae revealed that pathways involving neuroactive ligand–receptor interaction, lysosomes, and glycerolipid metabolism were associated with pre-diapause [ 20 ]. Comparative transcriptomic analysis of 11 insect species revealed that a set of core genes involved in circadian rhythmicity, insulin signalling, and Wnt signalling were differentially regulated during diapause [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Time-Series Transcriptomic Analysis Reveals the Molecular Profiles of Diapause Termination Induced by Long Photoperiods and High Temperature in Chilo suppressalis

Bao

Zhu

et al. 2022

IJMS

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Survival and adaptation to seasonal changes are challenging for insects. Many temperate insects such as the rice stem borer (Chilo suppressalis) overcome the adverse situation by entering diapause, wherein development changes dynamically occur and metabolic activity is suppressed. The photoperiod and temperature act as major environmental stimuli of diapause. However, the physiological and molecular mechanisms that interpret the ecologically relevant environmental cues in ontogenetic development during diapause termination are poorly understood. Here, we used genome-wide high-throughput RNA-sequencing to examine the patterns of gene expression during diapause termination in C. suppressalis. Major shifts in biological processes and pathways including metabolism, environmental information transmission, and endocrine signalling were observed across diapause termination based on over-representation analysis, short time-series expression miner, and gene set enrichment analysis. Many new pathways were identified in diapause termination including circadian rhythm, MAPK signalling, Wnt signalling, and Ras signalling, together with previously reported pathways including ecdysteroid, juvenile hormone, and insulin/insulin-like signalling. Our results show that convergent biological processes and molecular pathways of diapause termination were shared across different insect species and provided a comprehensive roadmap to better understand diapause termination in C. suppressalis.

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Enhancing bioactivity of Callistemon citrinus (Curtis) essential oil through novel nanoemulsion formulation

Haghbayan,

Moghimi,

Sarrafi

et al. 2024

Journal of Biomaterials Science, Polymer Edition

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Transcriptomic analysis of pre-diapause larvae of Chilo suppressalis (Walker) (Lepidoptera: Pyralidae) in natural populations

Cited by 4 publications

References 64 publications

Tachykinin-related peptides modulate immune-gene expression in the mealworm beetle Tenebrio molitor L.

Tachykinin-related peptides modulate immune-gene expression in the mealworm beetle Tenebrio molitor L.

Time-Series Transcriptomic Analysis Reveals the Molecular Profiles of Diapause Termination Induced by Long Photoperiods and High Temperature in Chilo suppressalis

Enhancing bioactivity of Callistemon citrinus (Curtis) essential oil through novel nanoemulsion formulation

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