Transcriptomic analysis of peripheral leukocytes in dairy cows with and without evidence of metritis and associated early postpartum disease

McConnel, Craig S.; Crisp, Sierra; Biggs, Tyler D.; Parrish, Lindsay M.; Sischo, William M.; Ficklin, Stephen P.

doi:10.15232/aas.2020-02092

Cited by 2 publications

(2 citation statements)

References 4 publications

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“…All the genes were found to be differentially expressed in two or more studies focused on BRD, and at least one study each that used RNA extracted from whole blood samples. Ten of the genes (CATHL6, CXCL8, DHX58, IFNG, IL17D, LCN2, LIF, PGLYRP1, SELP, and TNF) also were differentially expressed in dairy cattle diagnosed with metritis, mastitis, or Johne's Disease [26][27][28][29][30], and these genes have the potential to provide insight into conserved elements bridging disease states and target tissues in diverse dairy populations. Genes were prioritized that have strong evidence for their role in amplification or attenuation of inflammatory processes, the antibacterial or antiviral actions of their protein products, or their involvement in disease resistance and tolerance mechanisms.…”

Section: Gene Selectionmentioning

confidence: 99%

“…Multiple studies in feedlot cattle with BRD have identified common differentially expressed genes associated with disease presence, severity, and outcomes [14,15,19,21]. Likewise, studies aimed at identifying biomarkers of diseases affecting adult dairy cows such as mastitis, metritis, and Johne's disease have identified consistent gene expression changes associated with subclinical or clinical disease that may bridge disease states [28][29][30]. The differential expression patterns observed in these studies involving older animals were not observed in our cohort of pre-weaned calves, suggesting that there are additional factors affecting leukocyte behavior in young calves.…”

Section: Plos Onementioning

confidence: 99%

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Differential gene expression in peripheral leukocytes of pre-weaned Holstein heifer calves with respiratory disease

Elder,

Hinnant,

Mandella

et al. 2023

PLoS ONE

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Bovine respiratory disease (BRD) is a leading cause of calf morbidity and mortality, and prevalence remains high despite current management practices. Differential gene expression (DGE) provides detailed insight into individual immune responses and can illuminate enriched pathways and biomarkers that contribute to disease susceptibility and outcomes. The aims of this study were to investigate differences in peripheral leukocyte gene expression in Holstein preweaned heifer calves 1) with and without BRD, and 2) across weeks of age. Calves were enrolled for this short-term longitudinal study on two commercial dairies in Washington State. Calves were assessed every two weeks throughout the pre-weaning period using clinical respiratory scoring (CRS) and thoracic ultrasonography (TUS), and blood samples were collected. Calves were selected that were either healthy (n = 10) or had BRD diagnosed by CRS (n = 7), TUS (n = 6), or both (n = 6) in weeks 5 or 7 of life). Three consecutive time point samples were analyzed for each BRD calf consisting of PRE, ONSET, and POST samples. Nineteen genes of interest were selected based on previous gene expression studies in cattle: ALOX15, BPI, CATHL6, CXCL8, DHX58, GZMB, HPGD, IFNG, IL17D, IL1R2, ISG15, LCN2, LIF, MX1, OAS2, PGLYRP1, S100A8, SELP, and TNF. Comparisons were made between age and disease time point matched BRD and healthy calves as well as between calf weeks of age. No DGE was observed between diseased and healthy calves; however, DGE was observed between calf weeks of age regardless of disease state. Developmental differences in leukocyte gene expression, phenotype, and functionality make pre-weaned calves immunologically distinct from mature cattle, and early life shifts in calf leukocyte populations likely contribute to the age-related gene expression differences we observed. Age overshadows disease impacts to influence gene expression in young calves, and immune development progresses upon a common trajectory regardless of disease during the preweaning period.

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Section: Gene Selectionmentioning

confidence: 99%

Section: Plos Onementioning

confidence: 99%

Differential gene expression in peripheral leukocytes of pre-weaned Holstein heifer calves with respiratory disease

Elder,

Hinnant,

Mandella

et al. 2023

PLoS ONE

View full text Add to dashboard Cite

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Analysis of runs of homozygosity of cattle living in different climate zones

Falchi

Cesarani

Mastrangelo

et al. 2023

Journal of Animal Science

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Aim of this study was to analyze the distribution and characteristics of runs of homozygosity in Bos taurus taurus and Bos taurus indicus breeds, as well as their crosses, farmed all around the word. With this aim in view, we used SNP genotypes for 3,263 cattle belonging to 204 different breeds. After quality control, 23,311 SNPs were retained for the analysis. Animals were divided in seven different groups: 1) Continental taurus, 2) Temperate taurus, 3) Temperate indicus, 4) Temperate composite, 5) Tropical taurus, 6) Tropical indicus, and 7) Tropical composite. The climatic zones were created according to the latitude of their country of origin: i) Continental, latitude ≥ 45°; ii) Temperate, 45°< Latitude >23.26°; iii) Tropics, Latitude ≤ 23.26°. Runs of homozygosity were computed as 15 SNPs spanning in at least 2 Mb; number of ROH per animal (nROH), average ROH length (meanMb), and ROH-based inbreeding coefficients (FROH) were also computed. Temperate Indicus showed the largest nROH, whereas Temperate Taurus the lowest value. Moreover, the largest meanMb was observed for Temperate Taurus, whereas the lowest value for Tropics Indicus. Temperate Indicus breeds showed the largest FROH values. Genes mapped in the identified ROH were reported to be associated with the environmental adaptation, disease resistance, coat color determinism, and production traits. Results of the present study confirmed that runs of homozygosity could be used to identify genomic signatures due to both artificial and natural selection.

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Transcriptomic analysis of peripheral leukocytes in dairy cows with and without evidence of metritis and associated early postpartum disease

Cited by 2 publications

References 4 publications

Differential gene expression in peripheral leukocytes of pre-weaned Holstein heifer calves with respiratory disease

Differential gene expression in peripheral leukocytes of pre-weaned Holstein heifer calves with respiratory disease

Analysis of runs of homozygosity of cattle living in different climate zones

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