Transcriptomic analysis of metabolic changes by phosphorus stress in rice plant roots

Wasaki, Jun; Yonetani, Ryoma; Kuroda, Satoshi; Shinano, Takuro; Yazaki, Junshi; Fujii, Fumiko; Shimbo, Kanako; Yamamoto, Kimiko; Sakata, Katsumi; Sasaki, Takuji; Kikuchi, Shoshi; Yamagishi, Masumi; Osaki, Mitsuru

doi:10.1046/j.1365-3040.2003.01074.x

Cited by 223 publications

(198 citation statements)

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“…In contrast, no significant change in APase activity in L. albus under high [CO 2 ] and high P supply was observed (Wasaki et al, 2003). This reduction in activity could be attributed to the higher concentration of P in root tissues.…”

Section: Accepted M Manuscriptmentioning

confidence: 78%

“…24 under P deficiency (Wasaki et al, 2003) showed up-regulation of genes related to glycolysis, alteration in lipid metabolism, modification of cell wall synthesis and changes in the metal responsive genes. Similarly, some glycolytic pathway related genes were induced in roots of L. albus under P starvation (Uhde-Stone et al, 2003a, b).…”

Section: Accepted M Manuscriptmentioning

confidence: 99%

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Physiological and molecular alterations in plants exposed to high [CO2] under phosphorus stress

Pandey

Zinta

AbdElgawad

et al. 2015

Biotechnology Advances

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Physiological and molecular alterations in plants exposed to high [CO 2 ] under phosphorus stress, Biotechnology Advances (2015Advances ( ), doi: 10.1016Advances ( /j.biotechadv.2015 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. This is the author's version of a work that was accepted for publication in Biotechnology Advances (Ed. Elsevier). Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Pandey, Renu et al. Fax: +91-11-25846420] has increased substantially in recent decades and will continue to do so, whereas the availability of phosphorus (P) is limited and unlikely to increase in the future. P is a non-renewable resource, and it is essential to every form of life. P is a key plant nutrient controlling the responsiveness of photosynthesis to [CO 2 ]. Increases in [CO 2 ] typically results in increased biomass through stimulation of net photosynthesis, and hence enhance the demand for P uptake. However, most soils contain low concentrations of available P.Therefore, low P is one of the major growth-limiting factors for plants in many agricultural and natural ecosystems. The adaptive responses of plants to [CO 2 ] and P availability encompass alterations at morphological, physiological, biochemical and molecular levels. In general low P reduces growth, whereas high [CO 2 ] enhances it particularly in C 3 plants. Photosynthetic capacity is often enhanced under high [CO

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Section: Accepted M Manuscriptmentioning

confidence: 78%

Section: Accepted M Manuscriptmentioning

confidence: 99%

Physiological and molecular alterations in plants exposed to high [CO2] under phosphorus stress

Pandey

Zinta

AbdElgawad

et al. 2015

Biotechnology Advances

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“…Phosphorus is an element required for plant growth, and an essential component of many chemical substances in plants such as phospholipids, nucleic acids, ATP and NADP, and is closely related to many metabolic processes in plants [44,45]. Nutrient control experiments on rice show that after reducing the phosphorus content of soil, the expression of enzymes related to cell-wall-synthesis increased significantly while the expression of enzymes related to lipolysis decreased [46]. A study of pigeon pea (Cajanus cajan) using isotope labeling shows a decrease of soil phosphorus could significantly inhibit the accumulation of non-structural carbohydrates [47].…”

Section: Effect Of Environmental Factors On Forage-quality Patternsmentioning

confidence: 99%

Large scale patterns of forage yield and quality across Chinese grasslands

Shi

et al. 2012

Chin. Sci. Bull.

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Understanding the patterns of forage quantity and quality and investigating the factors influencing these patterns are essential for the development of animal husbandry. However, there is very little field evidence focused on these issues at a large spatial scale. In the current study, we analyzed forage quantity and quality at 177 sites distributed in all the major grassland types across China, and explored the relationship between forage quantity and quality based on consistent sampling protocols. We also investigated potential factors influencing forage quality patterns across China. Our study indicates the Tibetan grasslands had both higher quantity and quality forage than the Inner Mongolian grasslands, and alpine meadow had the best quantity and quality forage because of the meadow's high productivity and the crude protein and nitrogen free extract content of the meadow forage. For the main vegetation formations, Kobresia tibetica meadows and Achnatherum splendens steppes had the highest quantity, while Kobresia pygmaea meadows and Kobresia humilis meadows had the best quality. We also found that although environmental factors, such as temperature and soil fertility, could affect physiological processes and so influence forage quality, the large scale patterns of change were mainly a result of the differences in vegetation types. Finally, we reported a negative relationship between forage quantity and quality: higher forage quantity means more crude fiber but less ether extract and crude protein. These findings improve our understanding on the spatial patterns of forage quantity and quality, and provide solid evidence related to the future development of animal husbandry.

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“…The Pi starvation-induced reprogramming of architecture and metabolism is supported by vast changes in the plant transcriptome and has been demonstrated in several plant models (Wasaki et al, 2003;Chiou and Lin, 2011;Hu and Chu, 2011;Huang et al, 2011). In Arabidopsis, more than 600 genes are induced in response to Pi starvation (Misson et al, 2005;Thibaud et al, 2010).…”

mentioning

confidence: 99%

“…For instance, SQD1 and SQD2 are involved in sulfolipid biosynthesis, whereas MGD2 and MGD3 are strongly induced to increase galactolipid content (Yu et al, 2002;Jouhet et al, 2004;Nakamura et al, 2005;Kobayashi et al, 2009). To achieve these substitutions, phospholipids are degraded by different types of lipases, resulting in the release of lipid subcomponents that can reenter the metabolism or be converted into other lipid forms (Nakamura, 2013).The Pi starvation-induced reprogramming of architecture and metabolism is supported by vast changes in the plant transcriptome and has been demonstrated in several plant models (Wasaki et al, 2003;Chiou and Lin, 2011;Hu and Chu, 2011;Huang et al, 2011). In Arabidopsis, more than 600 genes are induced in response to Pi starvation (Misson et al, 2005;Thibaud et al, 2010).…”

mentioning

confidence: 99%

The Phosphate Fast-Responsive Genes PECP1 and PPsPase1 Affect Phosphocholine and Phosphoethanolamine Content

et al. 2018

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Phosphate starvation-mediated induction of the HAD-type phosphatases PPsPase1 (AT1G73010) and PECP1 (AT1G17710) has been reported in Arabidopsis (Arabidopsis thaliana). However, little is known about their in vivo function or impact on plant responses to nutrient deficiency. The preferences of PPsPase1 and PECP1 for different substrates have been studied in vitro but require confirmation in planta. Here, we examined the in vivo function of both enzymes using a reverse genetics approach. We demonstrated that PPsPase1 and PECP1 affect plant phosphocholine and phosphoethanolamine content, but not the pyrophosphate-related phenotypes. These observations suggest that the enzymes play a similar role in planta related to the recycling of polar heads from membrane lipids that is triggered during phosphate starvation. Altering the expression of the genes encoding these enzymes had no effect on lipid composition, possibly due to compensation by other lipid recycling pathways triggered during phosphate starvation. Furthermore, our results indicated that PPsPase1 and PECP1 do not influence phosphate homeostasis, since the inactivation of these genes had no effect on phosphate content or on the induction of molecular markers related to phosphate starvation. A combination of transcriptomics and imaging analyses revealed that PPsPase1 and PECP1 display a highly dynamic expression pattern that closely mirrors the phosphate status. This temporal dynamism, combined with the wide range of induction levels, broad expression, and lack of a direct effect on Pi content and regulation, makes PPsPase1 and PECP1 useful molecular markers of the phosphate starvation response.Plant growth is highly sensitive to a lack of phosphate (Pi); hence, the application of Pi fertilizers has become standard practice for high-throughput crop production (Cordell et al., 2009). Most phosphorus in the soil is not available to plants, as it is combined with other minerals or parts of organic compounds (Bieleski, 1973;Raghothama and Karthikeyan, 2005). Only a small fraction of soluble Pi (usually present at a concentration of ,10 mM in the soil) can be taken up by plants.Although growth is not optimal under limiting conditions, plants can withstand changing Pi concentrations within heterogeneous soils or in the external nutrient supply that can lead to reprogramming of their metabolism and architecture (Hammond et al., 2003;Péret et al., 2011;Plaxton and Tran, 2011). Root architecture can be modified to facilitate Pi uptake by favoring the development of lateral roots (at the expense of primary root elongation in many plants including Arabidopsis), increasing the density and length of root hairs, and limiting the development of aerial parts (López-Bucio et al., 2002;Svistoonoff et al., 2007;Gruber et al., 2013). Pi uptake mechanisms are enhanced at the root/soil interface, particularly through the stimulation of Pi transport activity (Mudge et al., 2002;Shin et al., 2004;Nussaume et al., 2011; Ayadi et al., 2015). In parallel, mobilization of Pi sources fr...

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Transcriptomic analysis of metabolic changes by phosphorus stress in rice plant roots

Cited by 223 publications

References 54 publications

Physiological and molecular alterations in plants exposed to high [CO2] under phosphorus stress

Physiological and molecular alterations in plants exposed to high [CO2] under phosphorus stress

Large scale patterns of forage yield and quality across Chinese grasslands

The Phosphate Fast-Responsive Genes PECP1 and PPsPase1 Affect Phosphocholine and Phosphoethanolamine Content

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