Transcriptomic analysis of grain filling in rice inferior grains under moderate soil drying

Wang, Guan-Qun; Li, Haoxuan; Feng, Lei; Chen, Moxian; Meng, Shuan; Ye, Nenghui; Zhang, Jianhua

doi:10.1093/jxb/erz010

Cited by 50 publications

(60 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The MD treatment applied at the middle to late stages of grain filling in rice enhanced the carbon reserve remobilization in the straws (Figure 1) and thus increased the grain weight (both the superior grain and inferior grain), especially that of the inferior grain (Wang, Li, Feng, et al, 2019;Yang & Zhang, 2006;Yang et al, 2001).The transport of prestored assimilates from rice straws to inferior grains in rice was increased, which resulted in increased inferior grain weight and improved rice yield (Wang, Li, Feng, et al, 2019;Yang & Zhang, 2006;Zhang et al, 2012). Therefore, a well-controlled balance between source and sink under MD treatment during grain filling is pivotal for rice production.…”

Section: Discussionmentioning

confidence: 99%

“…Grain filling in rice under MD treatment was enhanced by the efficient carbon flow from the straws, which was transported to grains to improve sink activity, thus increasing the inferior grain weight (Wang, Li, Feng, et al, 2019). The carbon flow translocated from the straws to grains, mainly referred to as sucrose.…”

Section: Discussionmentioning

confidence: 99%

“…The total RNA from straws and grains sampled at 12, 18, and 24 DAA under the CK and MD treatments was extracted using an RNeasy Plant Mini Kit (Qiagen). Then, libraries were constructed according to a method previously described (Wang, Li, Feng, et al, 2019). The libraries were then sequenced on an Illumina HiSeq4000 PE101 platform from both the 5′-and 3′-ends on the paired end.…”

Section: Rna Extraction and Library Construction Of Straws And Grainsmentioning

confidence: 99%

“…In the source-sink context, straws are considered as temporary source organs during grain filling, and grains are considered as sink organs. Previous work has shown that moderate soil drying, particularly when imposed during the middle to late stages of grain filling, could improve the transportation of carbon reserves from straws to grains, accelerate the grain filling rate, and increase grain weight, especially that of inferior grain, in both rice and wheat (Wang, Li, Feng, et al, 2019;Yang & Zhang, 2006;Yang, Zhang, Wang, & Zhu, 2001). The above described farm management practices can not only save the freshwater, but also increase the crop yield.…”

Section: Introductionmentioning

confidence: 99%

“…Starch accumulation in grains is therefore of great importance, because starch is the major constituent of the seeds (Panigrahi, Kariali, Panda, Lafarge, & Mohapatra, 2019;Yoshida, Forno, & Cock, 1971). The grain filling of inferior grains was significantly correlated with the carbon reserve remobilization from the straws under the moderate soil drying condition (Chen et al, 2013;Wang, Li, Feng, et al, 2019;Zhang et al, 2012). However, the balance control of carbon reserve remobilization between the straws and inferior grains in response to moderate soil drying is poorly understood.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Expression profile of the carbon reserve remobilization from the source to sink in rice in response to soil drying during grain filling

Wang

Gong

et al. 2020

Food and Energy Security

Self Cite

View full text Add to dashboard Cite

Moderate soil drying (MD) applied during the mid‐to‐late grain filling stages can promote carbon reserve remobilization in straws (sheaths and stems) and increase the grain weight. The coordination between starch‐to‐sucrose transition in straws and sucrose‐to‐starch conversion in inferior grains is essential for carbon reserve remobilization during grain filling. Herein, to reveal the regulating mechanism of carbon reserve remobilization from source to sink, RNA‐seq was utilized to analyze the dynamic transcript profile in source and sink of rice under MD treatment during grain filling. The expression of amylase genes and amylase activity was enhanced by MD treatment in straws, which is consistent with the increased amylase activity. In inferior grains, it was starch synthesis genes that were upregulated by MD treatment. Furthermore, an elevated ABA was found in both straws and inferior grains, which was caused by an increased expression of NCED1 and downregulation of ABA8OX2 by MD treatment, respectively. Additionally, the expression of MYB30, a transcription factor (TF) that negatively regulates beta‐amylase genes, was reduced in straws by MD, resulting in an increased amylase activity. In contrast, an increased expression of NAC activated the expressions of starch synthesis gene in inferior grains under MD. Therefore, it is MYB30 and NAC that cooperates in source and sink, respectively, to promote carbon reserve remobilization in response to MD. Taken together, genes involved in carbon flow from source to sink are different between rice straws and inferior grains.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Rna Extraction and Library Construction Of Straws And Grainsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Expression profile of the carbon reserve remobilization from the source to sink in rice in response to soil drying during grain filling

Wang

Gong

et al. 2020

Food and Energy Security

Self Cite

View full text Add to dashboard Cite

show abstract

Transcriptional analyses of maize leaves in response to high‐density planting

Cheng-hao

Chen

et al. 2022

Agronomy Journal

View full text Add to dashboard Cite

Maize (Zea mays L.) is one of the major cereal crops worldwide. Increasing planting density is an effective way to improve crop yield. However, plants grown under high‐density conditions compete for water, nutrients, and light, which often leads to changes in productivity. To date, few studies have determined the transcriptomic differences in maize leaves in response to different planting densities. This study examined the whole‐genome expression patterns in the leaves of maize planted under high and low densities to identify density‐regulated genes. Leaves at upper, ear, and lower stem nodes were collected at the grain‐filling stage of the maize hybrid Xianyu335 grown under low‐density planting and high‐density planting. In total, 72, 733, and 1,739 differentially expressed genes (DEGs) were identified in the respective upper, ear, and lower leaves under HDP. Upregulated and downregulated DEGs in the upper and lower leaves were similar in number, whereas upregulated DEGs in the ear leaves were significantly higher in number than the downregulated DEGs. Functional analysis indicated that genes responding to HDP‐related stresses were mediated by pathways involving four phytohormones responsible for metabolism and signaling, osmoprotectant biosynthesis, transcription factors, and fatty acid biosynthesis and protein kinases, which suggested that these pathways are affected by the adaptive responses of maize plants grown at high density. Research findings elucidated the molecular mechanisms underlying the physiological and biochemical responses of the leaves of maize planted at high density.

show abstract

Transcriptomic analysis of photosynthesis‐related genes regulated by alternate wetting and drying irrigation in flag leaves of rice

Song

Feng

Das

et al. 2020

Food and Energy Security

Self Cite

View full text Add to dashboard Cite

Alternate wetting and drying (AWD) irrigation has been widely used to replace continuous flooding (CF), which does not result in yield loss while improving water productivity. However, it is still unexplored as to how this agricultural practice would affect gene expression to control plant physiology. In this study, we found that photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr), chlorophyll (Chl) content, and leaf water potential (Lwp) significantly decreased while abscisic acid (ABA) content and water use efficiency (WUE) increased when soil water potential reached to −15 kPa in the AWD irrigation regime. We then analyzed the differences between AWD and CF irrigation practices in transcriptomic profiles for flag leaves. Genes were found to be differentially expressed in photosynthesis related pathways namely, Chl biosynthesis, photosynthesis‐antenna proteins pathway, photosynthesis process, and ABA signal transduction pathway. The study provides important information on the morphological adaptations observed in the flag leaves in response to AWD irrigation, which will act as a basic framework to conduct molecular studies for improving rice cultivation.

show abstract

Transcriptomic analysis of grain filling in rice inferior grains under moderate soil drying

Abstract: Carbohydrate accumulation in inferior grains in rice panicles subjected to moderate post-anthesis water stress is regulated by NAC transcription factors that activate the expression of WAXY.

Cited by 50 publications

References 72 publications

Expression profile of the carbon reserve remobilization from the source to sink in rice in response to soil drying during grain filling

Expression profile of the carbon reserve remobilization from the source to sink in rice in response to soil drying during grain filling

Transcriptional analyses of maize leaves in response to high‐density planting

Transcriptomic analysis of photosynthesis‐related genes regulated by alternate wetting and drying irrigation in flag leaves of rice

Contact Info

Product

Resources

About