Transcriptomic analysis of flower opening response to relatively low temperatures in Osmanthus fragrans

Fu, Jianxin; Zhang, Chao; Liu, Yucheng; Pang, Tianhong; Dong, Bin; Gao, Xiaoyue; Zhu, Yimin; Zhao, Hongbo

doi:10.21203/rs.3.rs-24581/v1

Cited by 2 publications

(7 citation statements)

References 56 publications

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“…In this work (Fig. 9), ERF, Trihelix, WRKY and C3H family members were identified in chrysanthemum, and were upregulated during chrysanthemum flower bud differentiation or development [35][36][37][38][39][40][41]. This implies that these genes may be involved in flower developmental processes, and these TFs should be further studied in chrysanthemum.…”

Section: Transcription Factorsmentioning

confidence: 62%

“…RNA-seq technology was used to explore the effects of hydroponic culture on chrysanthemum flower. Transcriptome sequencing was used to analyze the flower, leaf and root under light, salinity and other conditions of chrysanthemum, and many key genes were also found [35][36][37][38][39][40][41][42][43][44][45][46][47][48][49][50][51]. Through KEGG and GO enrichment analysis, key genes and biological pathways were screened to comprehensively analyze the effects of hydroponic culture on gene expression and regulatory networks.…”

Section: Discussionmentioning

confidence: 99%

“…9). Transcription factors make important contributions to the regulation of plant growth and development, and can also influence plant secondary metabolites through orchestrating regulatory networks [33][34][35][36][37][38][39][40][41][42][43][44] In the present study, 26 and 7 C2H2 genes were up-and down-regulated, respectively, in flowers grown in a hydroponic system, implying that C2H2 TFs are important for chrysanthemum flower development or the biosynthesis of flavonoids and chlorogenic acid. MYB and bHLH transcription factors are important regulators involved in controlling plant or flower growth and development, and are involved in secondary metabolites synthesis in plants [81][82][83][84].…”

Section: Transcription Factorsmentioning

confidence: 91%

“…In the current study, many members of different TF families, such as WRKY, MYB, MYC, NAC, C3H, Trihelix, C2H2, ERF and CRT TFs were upregulated during flower bud differentiation or development of chrysanthemum flower [35][36][37][38][39][40]. In sweet osmanthus (Osmanthus fragras Lour), low temperature increased the expression of genes related to cell expansion (encoding Expansin, Xyloglucan endotransglycosylase/hydrolase, Xylosidase, Polygalacturonase) and phytohormones (Auxin, Gibberellic acid, Ethylene, and Brassinosteroids) to affect flowering time [41]. Transcription of expansin genes increased during flower opening of wintersweet (Chimonanthus paraecox) [42].…”

Section: Introductionmentioning

confidence: 99%

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Comparison of chrysanthemum flowers grown under hydroponic and soil-based systems: yield and transcriptome analysis

Ai¹,

Liu²,

Li³

et al. 2021

BMC Plant Biol

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Background Flowers of Chrysanthemum × morifolium Ramat. are used as tea in traditional Chinese cuisine. However, with increasing population and urbanization, water and land availability have become limiting for chrysanthemum tea production. Hydroponic culture enables effective, rapid nutrient exchange, while requiring no soil and less water than soil cultivation. Hydroponic culture can reduce pesticide residues in food and improve the quantity or size of fruits, flowers, and leaves, and the levels of active compounds important for nutrition and health. To date, studies to improve the yield and active compounds of chrysanthemum have focused on soil culture. Moreover, the molecular effects of hydroponic and soil culture on chrysanthemum tea development remain understudied. Results Here, we studied the effects of soil and hydroponic culture on yield and total flavonoid and chlorogenic acid contents in chrysanthemum flowers (C. morifolium ‘wuyuanhuang’). Yield and the total flavonoids and chlorogenic acid contents of chrysanthemum flowers were higher in the hydroponic culture system than in the soil system. Transcriptome profiling using RNA-seq revealed 3858 differentially expressed genes (DEGs) between chrysanthemum flowers grown in soil and hydroponic conditions. Gene Ontology (GO) enrichment annotation revealed that these differentially transcribed genes are mainly involved in “cytoplasmic part”, “biosynthetic process”, “organic substance biosynthetic process”, “cell wall organization or biogenesis” and other processes. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed enrichment in “metabolic pathways”, “biosynthesis of secondary metabolites”, “ribosome”, “carbon metabolism”, “plant hormone signal transduction” and other metabolic processes. In functional annotations, pathways related to yield and formation of the main active compounds included phytohormone signaling, secondary metabolism, and cell wall metabolism. Enrichment analysis of transcription factors also showed that under the hydroponic system, bHLH, MYB, NAC, and ERF protein families were involved in metabolic pathways, biosynthesis of secondary metabolites, and plant hormone signal transduction. Conclusions Hydroponic culture is a simple and effective way to cultivate chrysanthemum for tea production. A transcriptome analysis of chrysanthemum flowers grown in soil and hydroponic conditions. The large number of DEGs identified confirmed the difference of the regulatory machinery under two culture system.

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Section: Transcription Factorsmentioning

confidence: 62%

Section: Discussionmentioning

confidence: 99%

Section: Transcription Factorsmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Comparison of chrysanthemum flowers grown under hydroponic and soil-based systems: yield and transcriptome analysis

Ai¹,

Liu²,

Li³

et al. 2021

BMC Plant Biol

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show abstract

“…), a member of the Oleaceae family, is cultivated as a famous ornamental plant in China (Wang et al, 2022). According to flowering characteristics, sweet osmanthus is divided into OF habit, such as 'Yanhonggui', and CF habit, like 'Sijigui' (Fu et al, 2020). In contrast to OF habit, which only flowers in autumn, CF habit can bloom continuously under favorable environmental conditions (Fu et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Genetic studies on continuous flowering in woody plant Osmanthus fragrans

Wang

Gao²,

Chen³

et al. 2022

Front. Plant Sci.

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Continuous flowering is a key horticultural trait in ornamental plants, whereas the specific molecular regulation mechanism remains largely unknown. In sweet osmanthus (Osmanthus fragrans Lour.), plants based on their flowering characteristics are divided into once-flowering (OF) habit and continuous flowering (CF) habit. Here, we first described the flowering phenology shifts of OF and CF habits in sweet osmanthus through paraffin section and microscope assay. Phenotypic characterization showed that CF plants had constant new shoot growth, floral transition, and blooming for 1 year, which might lead to a continuous flowering trait. We performed the transcriptome sequencing of OF and CF sweet osmanthus and analyzed the transcriptional activity of flowering-related genes. Among the genes, three floral integrators, OfFT, OfTFL1, and OfBFT, had a differential expression during the floral transition process in OF and CF habits. The expression patterns of the three genes in 1 year were revealed. The results suggested that their accumulations corresponded to the new shoots occurring and the floral transition process. Function studies suggested that OfFT acted as a flowering activator, whereas OfBFT was a flowering inhibitor. Yeast one-hybrid assay indicated that OfSPL8 was a common upstream transcription factor of OfFT and OfBFT, suggesting the vital role of OfSPL8 in continuous flowering regulation. These results provide a novel insight into the molecular mechanism of continuous flowering.

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Transcriptomic analysis of flower opening response to relatively low temperatures in Osmanthus fragrans

Cited by 2 publications

References 56 publications

Comparison of chrysanthemum flowers grown under hydroponic and soil-based systems: yield and transcriptome analysis

Comparison of chrysanthemum flowers grown under hydroponic and soil-based systems: yield and transcriptome analysis

Genetic studies on continuous flowering in woody plant Osmanthus fragrans

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