Transcriptomic analysis of female and male gonads in juvenile snakeskin gourami (Trichopodus pectoralis)

Boonanuntanasarn, Surintorn; Jangprai, Araya; Na-Nakorn, Uthairat

doi:10.1038/s41598-020-61738-0

Cited by 17 publications

(10 citation statements)

References 65 publications

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“…All of the DEGs discovered in this inquiry were annotated using a variety of functional databases. We found that 11.12% of the assembled unigenes had a substantial annotation to all databases (NR, Swiss-Prot, Pfam, COG, GO, and KEGG), which was significantly higher than previous studies of juvenile snakeskin gourami female and male gonads, which showed 9.8% of assembled matching (Boonanuntanasarn et al, 2020). When the DEGs were enriched in the KEGG database, certain pathways relevant to the development of fish ovary were discovered including the Wnt signaling pathway and the GnRH signaling pathway, and this was consistent with a previous study by H. Liu et al (2019).…”

Section: Transcriptome Assembly and Annotation Analysiscontrasting

confidence: 72%

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Comparative transcriptome analysis reveals differentially expressed genes and signaling pathways between male and female mature gonads of Hemibarbus maculatus

Zhong

Lian

Chen

et al. 2022

J World Aquaculture Soc

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Hemibarbus maculatus is a globally important species for its value in fisheries and aquaculture. There is relatively little information available about this species' sexual development genes. Therefore, in this study, we used the technology of high‐throughput RNA sequencing (RNA‐seq), to investigate if the ovary and testis' differently expressed genes (DEGs) during sex differentiation in H. maculatus. Our results revealed a total of 43,468,248 and 39,241,362 qualified reads were generated in the female and male libraries, and 32,127 unigenes were assembled via the annotation analysis. Moreover, 17,808 unigenes were found to be DEGs between males and females, with 12,726 up‐ and 5082 downregulated genes in the ovaries and testes, respectively. The transcriptome analysis revealed there are 23 DEGs between ovary and testes of H. maculatus, which may be involved in sex determination. The expression patterns of these genes were validated by quantitative real‐time PCR (qPCR). The results of qPCR were generally consistent with the RNA‐seq results. This work was the first to annotate genes linked with gonadal development and reproduction in H. maculatus using gonadal transcriptome analysis, which provides useful molecular data for a better understanding of sexual development, as well as functional genomics and population genetics studies of H. maculatus.

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Section: Transcriptome Assembly and Annotation Analysiscontrasting

confidence: 72%

“…Sex differentiation is considered to be the most significant factor in the evolution of species (Boonanuntanasarn et al, 2020; Taboada et al, 2014). A fascinating scientific problem, sex determination and differentiation may influence biological reproduction and cause sexual morphism.…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptome analysis reveals differentially expressed genes and signaling pathways between male and female mature gonads of Hemibarbus maculatus

Zhong

Lian

Chen

et al. 2022

J World Aquaculture Soc

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“…Furthermore, in the gonad of the olive flounder, Paralichthys olivaceus, PIWI-related gene expression was 2,000 fold higher in the testis compared to the ovary (Zhang et al, 2016). Similar differences in PIWI-related genes were corroborated in the gonads of turbot (Scophthalmus Maximus), snakeskin gourami (Trichopodus pectoralis), and yellowfin seabream (Acanthopagrus latus) (Wang et al, 2018;Boonanuntanasarn et al, 2020;Li et al, 2020).…”

Section: Sex Differences In Transposable Elementsmentioning

confidence: 77%

The Role of Transposable Elements in Sexual Development

Chiang

DeRosa

Park

et al. 2022

Front. Behav. Neurosci.

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Up to 50% of most mammalian genomes are made up of transposable elements (TEs) that have the potential to mobilize around the genome. Despite this prevalence, research on TEs is only beginning to gain traction within the field of neuroscience. While TEs have long been regarded as “junk” or parasitic DNA, it has become evident that they are adaptive DNA and RNA regulatory elements. In addition to their vital role in normal development, TEs can also interact with steroid receptors, which are key elements to sexual development. In this review, we provide an overview of the involvement of TEs in processes related to sexual development- from TE activity in the germline to TE accumulation in sex chromosomes. Moreover, we highlight sex differences in TE activity and their regulation of genes related to sexual development. Finally, we speculate on the epigenetic mechanisms that may govern TEs’ role in sexual development. In this context, we emphasize the need to further the understanding of sexual development through the lens of TEs including in a variety of organs at different developmental stages, their molecular networks, and evolution.

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“…Examples of this are the Spats1 homolog from bony fish Oryzias latipes (GenBank Access number FS557457), cartilaginous fish Callorhinchus mili (access number XP_007901502.1), cephalochordates Branchiostoma belcheri and Branchiostoma floridae (access numbers XP_019647808 and XP_002590276.1, respectively), hemichordate Saccoglossus kowalevskii (access number XP_002731024.1), echinoderm Strongylocentrotus purpuratus (access number XP_001184069.1), brachiopod Lingula anatina (access number XP_013389114.1), and the mollusk Octopus bimaculoides (access number XP_014774497.1), among others. Of note, a recent study in juvenile snakeskin gourami fish also identified Spats1 among the top differentially expressed genes in testis, as compared to ovary [ 35 ].…”

Section: Resultsmentioning

confidence: 99%

SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse

et al. 2021

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SPATS1 (spermatogenesis-associated, serine-rich 1) is an evolutionarily conserved, testis-specific protein that is differentially expressed during rat male meiotic prophase. Some reports have suggested a link between SPATS1 underexpression/mutation and human pathologies such as male infertility and testicular cancer. Given the absence of functional studies, we generated a Spats1 loss-of-function mouse model using CRISPR/Cas9 technology. The phenotypic analysis showed no overt phenotype in Spats1-/- mice, with both males and females being fertile. Flow cytometry and histological analyses did not show differences in the testicular content and histology between WT and knockout mice. Moreover, no significant differences in sperm concentration, motility, and morphology, were observed between WT and KO mice. These results were obtained both for young adults and for aged animals. Besides, although an involvement of SPATS1 in the Wnt signaling pathway has been suggested, we did not detect changes in the expression levels of typical Wnt pathway-target genes in mutant individuals. Thus, albeit Spats1 alteration might be a risk factor for male testicular health, we hereby show that this gene is not individually essential for male fertility and spermatogenesis in mouse.

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Transcriptomic analysis of female and male gonads in juvenile snakeskin gourami (Trichopodus pectoralis)

Cited by 17 publications

References 65 publications

Comparative transcriptome analysis reveals differentially expressed genes and signaling pathways between male and female mature gonads of Hemibarbus maculatus

Comparative transcriptome analysis reveals differentially expressed genes and signaling pathways between male and female mature gonads of Hemibarbus maculatus

The Role of Transposable Elements in Sexual Development

SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse

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