Transcriptomic analysis of changes in gene expression of immune proteins of gill tissue in response to low environmental temperature in fathead minnows ( Pimephales promelas )

Wentworth, Simon; Thede, Katrina; Aravindabose, Varsha; Monroe, Ian; Thompson, Andrew; Molyneaux, Neil; Owen, Christopher L.; Burns, John R.; Gonzalez‐Vicente, Agustin; Garvin, Jeffrey L.; Packer, Randall K.

doi:10.1016/j.cbd.2017.11.004

Cited by 10 publications

(8 citation statements)

References 36 publications

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“…In Crucian carp and goldfish with temperature tolerances of 2-22 • C and 0-41 • C, respectively, hypertrophy of interlamellar cells was observed after a 30-day exposure to 7.5 • C [47]. In fathead minnow (Pimephales promela) with temperature tolerance of 0-33 • C acclimated to 5 • C, no changes occurred in interlamellar cells, although mucus cells in the gills were observed to be fewer but larger after acclimation [48].…”

Section: Temperaturementioning

confidence: 96%

“…Lysozyme activity, hepcidin, and immunoglobulin M (IgM) increased in the skin of turbot (Scophthalmus maximus), with a temperature tolerance of 16-20 • C after exposure to 27 • C [49]. Genes coding for proteins that block viral replication in the early phase, macrophage activation, mucus secretion, and pro-inflammatory responses were upregulated while the genes coding for antigen presentation and immunoglobulins were downregulated in fathead minnow acclimated to 5 • C [48]. In channel catfish, the adaptive immune response to T-dependent antigens in the gills is inhibited by low temperature [50].…”

Section: Temperaturementioning

confidence: 99%

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Mucosal Barrier Functions of Fish under Changing Environmental Conditions

Cabillon¹,

Lazado

2019

Fishes

112

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The skin, gills, and gut are the most extensively studied mucosal organs in fish. These mucosal structures provide the intimate interface between the internal and external milieus and serve as the indispensable first line of defense. They have highly diverse physiological functions. Their role in defense can be highlighted in three shared similarities: their microanatomical structures that serve as the physical barrier and hold the immune cells and the effector molecules; the mucus layer, also a physical barrier, contains an array of potent bioactive molecules; and the resident microbiota. Mucosal surfaces are responsive and plastic to the different changes in the aquatic environment. The direct interaction of the mucosa with the environment offers some important information on both the physiological status of the host and the conditions of the aquatic environment. Increasing attention has been directed to these features in the last year, particularly on how to improve the overall health of the fish through manipulation of mucosal functions and on how the changes in the mucosa, in response to varying environmental factors, can be harnessed to improve husbandry. In this short review, we highlight the current knowledge on how mucosal surfaces respond to various environmental factors relevant to aquaculture and how they may be exploited in fostering sustainable fish farming practices, especially in controlled aquaculture environments.

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Section: Temperaturementioning

confidence: 96%

Section: Temperaturementioning

confidence: 99%

Mucosal Barrier Functions of Fish under Changing Environmental Conditions

Cabillon¹,

Lazado

2019

Fishes

112

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“…Variation in temperature range is being an environmental stress factor which affects cellular homeostasis and results in growth retardation and also affects reproduction and leads to variations in antioxidants content of seafood (Guerriero et al, 2002;Dong et al, 2008;Wentworth et al, 2018). The heat shock response (HSR) is known to be a possible cellular technique which may affect the upper thermal tolerance of any organism (Lee et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Temperature changes and marine fish species (Epinephelus coioides and Sparus aurata): Role of oxidative stress biomarkers in toxicological food studies

et al. 2018

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The increase of seawater temperature as a result of global climate variation elucidates a major challenge for marine organisms survival in addition to consumers safety. Spotted grouper (Epinephelus coioides) and Seabream (Sparus aurata) were collected in water with different temperature variations at Suez Canal and Alexandria (Suez and Abu Qir bay) in Egypt with the aim to assess expression levels of heat shock proteins such as HSP47, HSP70 and HSP90 genes in addition to antioxidants value through enzymes activity: Glutathione-S-Transferase (GST) and Glutathione Peroxidase (GPx). Research results revealed that expression of the HSP47, HSP70a and HSP90 genes increased in marine fishes tissues collected from Suez Canal, with higher water temperature (23:28ºC), compared with those collected from Alexandria (19:24°C) whereas the content of GPx and GST decreased. Our results show alteration of the marker examined suggesting that the increase of heat shock protein genes expression levels of fish collected from Suez Canal might be exposed mainly to thermal oxidative stress response more than those collect from Alexandria. The increase of heat shock protein-related genes expression could be considered as a factor in prohibiting the heat shock transcription factor that may lead to stimulation of heat-inducible genes in addition to heat acclimation. Thus, warming of water is also likely to alter the composition and abundance of food resources, e.g. fish muscles, available to higher trophic level consumers.

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“…Numerous studies have characterized the transcriptional changes in various fishes as they respond to cold stress and found they exhibited specific physiological responses to their acclimation during low-temperature stress [3][4][5]. For instance, several main pathways, including carbohydrate metabolism, lipid metabolism, immune system, and signal transduction pathways, were significantly enriched in many fishes [6][7][8][9][10]. In contrast, Sparus aurata and Danio rerio have been resistant to the adverse effects of cold stress through different functional regulations like antioxidant responses, blood glucose regulation, and membrane fluidity [5,11].…”

Section: Introductionmentioning

confidence: 99%

Gene Expression Profile and Co-Expression Network of Pearl Gentian Grouper under Cold Stress by Integrating Illumina and PacBio Sequences

Miao

Niu

et al. 2021

Animals

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Pearl gentian grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂) is a fish of high commercial value in the aquaculture industry in Asia. However, this hybrid fish is not cold-tolerant, and its molecular regulation mechanism underlying cold stress remains largely elusive. This study thus investigated the liver transcriptomic responses of pearl gentian grouper by comparing the gene expression of cold stress groups (20, 15, 12, and 12 °C for 6 h) with that of control group (25 °C) using PacBio SMRT-Seq and Illumina RNA-Seq technologies. In SMRT-Seq analysis, a total of 11,033 full-length transcripts were generated and used as reference sequences for further RNA-Seq analysis. In RNA-Seq analysis, 3271 differentially expressed genes (DEGs), two low-temperature specific modules (tan and blue modules), and two significantly expressed gene sets (profiles 0 and 19) were screened by differential expression analysis, weighted gene co-expression networks analysis (WGCNA), and short time-series expression miner (STEM), respectively. The intersection of the above analyses further revealed some key genes, such as PCK, ALDOB, FBP, G6pC, CPT1A, PPARα, SOCS3, PPP1CC, CYP2J, HMGCR, CDKN1B, and GADD45Bc. These genes were significantly enriched in carbohydrate metabolism, lipid metabolism, signal transduction, and endocrine system pathways. All these pathways were linked to biological functions relevant to cold adaptation, such as energy metabolism, stress-induced cell membrane changes, and transduction of stress signals. Taken together, our study explores an overall and complex regulation network of the functional genes in the liver of pearl gentian grouper, which could benefit the species in preventing damage caused by cold stress.

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Transcriptomic analysis of changes in gene expression of immune proteins of gill tissue in response to low environmental temperature in fathead minnows ( Pimephales promelas )

Cited by 10 publications

References 36 publications

Mucosal Barrier Functions of Fish under Changing Environmental Conditions

Mucosal Barrier Functions of Fish under Changing Environmental Conditions

Temperature changes and marine fish species (Epinephelus coioides and Sparus aurata): Role of oxidative stress biomarkers in toxicological food studies

Gene Expression Profile and Co-Expression Network of Pearl Gentian Grouper under Cold Stress by Integrating Illumina and PacBio Sequences

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