Abstract:Background
Hypoxia mediated pulmonary hypertension (HPH) is a lethal disease and lacks effective therapy. CircRNAs play significant roles in physiological process. Recently, circRNAs are found to be m6A-modified. The abundance of circRNAs was influenced by m6A. Furthermore, the significance of m6A circRNAs has not been elucidated in HPH yet. Here we aim to investigate the transcriptome-wide map of m6A circRNAs in HPH.
Results
Differentially express… Show more
“…Furthermore, the network of circRNA/miRNA/mRNA is also regulated by m 6 A in HPH. CircXpo6 and circTmtc3 are both m 6 A-modified and then down-regulated in HPH 162 .…”
Section: The Interplay Between M
6
a And Other Epimentioning
Epigenetic regulation involves a range of sophisticated processes which contribute to heritable alterations in gene expression without altering DNA sequence. Regulatory events predominantly include DNA methylation, chromatin remodeling, histone modifications, non-coding RNAs (ncRNAs), and RNA modification. As the most prevalent RNA modification in eukaryotic cells, N
6
-methyladenosine (m
6
A) RNA methylation actively participates in the modulation of RNA metabolism. Notably, accumulating evidence has revealed complicated interrelations occurring between m
6
A and other well-known epigenetic modifications. Their crosstalk conspicuously triggers epigenetic remodeling, further yielding profound impacts on a variety of physiological and pathological processes, especially tumorigenesis. Herein, we provide an up-to-date review of this emerging hot area of biological research, summarizing the interplay between m
6
A RNA methylation and other epigenetic regulators, and highlighting their underlying functions in epigenetic reprogramming.
“…Furthermore, the network of circRNA/miRNA/mRNA is also regulated by m 6 A in HPH. CircXpo6 and circTmtc3 are both m 6 A-modified and then down-regulated in HPH 162 .…”
Section: The Interplay Between M
6
a And Other Epimentioning
Epigenetic regulation involves a range of sophisticated processes which contribute to heritable alterations in gene expression without altering DNA sequence. Regulatory events predominantly include DNA methylation, chromatin remodeling, histone modifications, non-coding RNAs (ncRNAs), and RNA modification. As the most prevalent RNA modification in eukaryotic cells, N
6
-methyladenosine (m
6
A) RNA methylation actively participates in the modulation of RNA metabolism. Notably, accumulating evidence has revealed complicated interrelations occurring between m
6
A and other well-known epigenetic modifications. Their crosstalk conspicuously triggers epigenetic remodeling, further yielding profound impacts on a variety of physiological and pathological processes, especially tumorigenesis. Herein, we provide an up-to-date review of this emerging hot area of biological research, summarizing the interplay between m
6
A RNA methylation and other epigenetic regulators, and highlighting their underlying functions in epigenetic reprogramming.
“…m6A facilitates cytoplasmic export of circRNAs, drives circRNA translation, and mediates circRNA degradation. 32 , 33 Previous reviews have summarized the role of m6A modification in the regulation of ncRNA, including the important processes of controlling RNA functions, including processing, stabilization, and transportation, and the crosstalk between RNA m6A-modified and ncRNA in promoting tumor growth and progression. 34 Figure 1 m6A-modifying process on RNAs m6A receives deposition onto RNAs by the m6A writing element, including WTAP, METTL14, METTL3, VIRMA, HAKA1, RBM15/15B, and ZC3H13.…”
Section: Epigenetic Types Of Ncrna Modificationsmentioning
As next-generation sequencing (NGS) is leaping forward, more than 160 covalent RNA modification processes have been reported, and they are widely present in every organism and overall RNA type. Many modification processes of RNA introduce a new layer to the gene regulation process, resulting in novel RNA epigenetics. The commonest RNA modification includes pseudouridine (Ψ),
N
7
-methylguanosine (m7G), 5-hydroxymethylcytosine (hm5C), 5-methylcytosine (m5C),
N
1
-methyladenosine (m1A),
N
6
-methyladenosine (m6A), and others. In this study, we focus on non-coding RNAs (ncRNAs) to summarize the epigenetic consequences of RNA modifications, and the pathogenesis of cancer, as diagnostic markers and therapeutic targets for cancer, as well as the mechanisms affecting the immune environment of cancer. In addition, we summarize the current status of epigenetic drugs for tumor therapy based on ncRNA modifications and the progress of bioinformatics methods in elucidating RNA modifications in recent years.
“…In human embryonic stem cells (hESCs) and HeLa cells, Zhou et al (2017) identified 1,404 m6A circRNAs and 987 m6A circRNAs, respectively, and they found that m6A circRNAs were expressed in cell-type-specific methylation patterns. In a rat model of hypoxia mediated pulmonary hypertension (HPH), Su et al (2020) identified 1130 m6A circRNAs in total. In the lens epithelium cells from age-related cataract (ARC), Li et al (2020) identified 4876 m6A peaks within circRNAs.…”
Section: M6a Modification Of Circrna Widely Existed M6a Modification Of Circrnasmentioning
confidence: 99%
“…Although it was not until 2017 that circRNAs were found to be modified by m6A, many studies have shown that m6A modification of circRNAs plays important roles in physiological processes and disease progressions (Figure 3), such as tumors (Chen R. X. et al, 2019;Chen et al, 2021;Li et al, 2021), acute coronary syndrome (Guo et al, 2020), pulmonary hypertension (Su et al, 2020), and age-related cataract (Li et al, 2020). In this section, we focused on the roles of m6A-modified circRNAs in physiological processes and disease progressions.…”
As the most abundant internal modification in eukaryotic cells, N6-methyladenosine (m6A) in mRNA has shown widespread regulatory roles in a variety of physiological processes and disease progressions. Circular RNAs (circRNAs) are a class of covalently closed circular RNA molecules and play an essential role in the pathogenesis of various diseases. Recently, accumulating evidence has shown that m6A modification is widely existed in circRNAs and found its key biological functions in regulating circRNA metabolism, including biogenesis, translation, degradation and cellular localization. Through regulating circRNAs, studies have shown the important roles of m6A modification in circRNAs during immunity and multiple diseases, which represents a new layer of control in physiological processes and disease progressions. In this review, we focused on the roles played by m6A in circRNA metabolism, summarized the regulatory mechanisms of m6A-modified circRNAs in immunity and diseases, and discussed the current challenges to study m6A modification in circRNAs and the possible future directions, providing a comprehensive insight into understanding m6A modification of circRNAs in RNA epigenetics.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
