Transcriptome-wide identification of microRNAs in response to 20-hydroxyecdysone in Galeruca daurica

Wang, Haichao; Duan, Tian-Feng; Li, Ling; Pang, Bao-Ping

doi:10.1016/j.cbd.2022.100981

Cited by 1 publication

(2 citation statements)

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“…34,35 Previous studies on this pest have mainly focused on host plants, 35 life history, 36 genetic diversity, 37,38 cold resistance, [39][40][41][42] olfaction, [43][44][45] diapause, [46][47][48][49][50] and miRNAs. [51][52][53] More recently, Duan et al verified that two miR-NAs, let-7-5p and miR-2765-3p, modulated the reproductive diapause of this beetle through targeting Kr-h1 and FoxO, respectively. 54,55 However, there has been no report on metamorphosis in G. daurica to date.…”

Section: Introductionmentioning

confidence: 97%

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MicroRNA miR‐285 modulates the metamorphosis in Galeruca daurica by targeting Br‐C

Wang,

Li,

Zhang

et al. 2024

Pest Management Science

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BACKGROUDGaleruca daurica has become a new pest on the Inner Mongolia grasslands since its abrupt outbreak in 2009, and caused serious damage. As a pupa indicator during insect metamorphosis, the early response gene of ecdysone signaling pathway, Broad‐Complex (Br‐C), plays a vital role in the growth and development of insects. MicroRNAs (miRNA) are small non‐coding RNAs which mediate various biological activities. However, it is unknown whether and how Br‐C is regulated by miRNAs.RESULTSTemporal expression profiles revealed that miR‐285 and Br‐C basically displayed an opposite trend during the larval‐adult development, and Br‐C was sharply up‐regulated on the last day of final instar larvae meanwhile miR‐285 was significantly down‐regulated. Both dual‐luciferase reporter assay and miRNA‐mRNA interaction assay indicated that miR‐285 interacts with the coding sequence of Br‐C and represses its expression. Not only overexpression but also downexpression of miR‐285 led to the failure of larval to pupal to adult metamorphosis. In addition, both overexpression of miR‐285 and silence of Br‐C inhibited the expression of Br‐C and other ecdysone signaling pathway genes including E74, E75, ECR, FTZ‐F1, and HR3. On the contrary, suppressing miR‐285 obtained opposite results. Further experiments showed that 20‐hydroxyecdysone down‐regulated miR‐285, and up‐regulated Br‐C and above‐mentioned genes whereas JHA resulted in opposite effects.CONCLUSIONOur results reveal that miR‐285 is involved in mediating the metamorphosis in G. daurica by targeting Br‐C in the ecdysone signaling pathway. miR‐285 and its target Br‐C could be as a potential target for G. daurica management.

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Section: Introductionmentioning

confidence: 97%

“…Since its abrupt outbreak in 2009, it has caused serious damage to grasslands 34,35 . Previous studies on this pest have mainly focused on host plants, 35 life history, 36 genetic diversity, 37,38 cold resistance, 39–42 olfaction, 43–45 diapause, 46–50 and miRNAs 51–53 . More recently, Duan et al .…”

Section: Introductionmentioning

confidence: 99%