Transcriptome-wide identification of 5-methylcytosine by deaminase and reader protein-assisted sequencing
Jiale Zhou,
Ding Zhao,
Jinze Li
et al.
Abstract:5-Methylcytosine (m5C) is one of the major post-transcriptional modifications in mRNA and is highly involved in the pathogenesis of various diseases. However, the capacity of existing assays for accurately and comprehensively transcriptome-wide m5C mapping still needs improvement. Here, we develop a detection method named DRAM (deaminase and reader protein assisted RNA methylation analysis), in which deaminases (APOBEC1 and TadA-8e) are fused with m5C reader proteins (ALYREF and YBX1) to identify the m5C sites… Show more
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