Transcriptome-wide identification and expression analyses of ABC transporters in dwarf polish wheat under metal stresses

Wang, X.; Wang, Chang Quan; Sheng, Huajin; Wang, Y.; Zeng, Jian; Kang, Houyang; Fan, Xing; Li, Sha; Zhang, H.; Zhou, Yonghong

doi:10.1007/s10535-016-0697-0

Cited by 29 publications

(14 citation statements)

References 57 publications

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“…We grouped these proteins into different interactions of Cd/Zn (Figure 2 ). Our results indicate that although Cd and Zn have similar physical and chemical properties (Chesworth, 1991 ) and pathways for uptake (Grant et al, 1998 ), they induce differential molecular responses (Lin and Arats, 2012 ), which result in the antagonistic interactions of Cd/Zn in DPW roots (Figure 1 ) and the high tolerance of DPW to Cd and Zn toxicity (Wang X. et al, in press ). Some proteins identified in this study that are involved in noteworthy processes are discussed below.…”

Section: Discussionmentioning

confidence: 75%

“…Polish wheat (2 n = 4x = 28, AABB, Triticum polonicum L.), which has low genetic similarity with T. aestivum (Wang et al, 2013 ; Michalcová et al, 2014 ), accumulates high concentrations of Zn, Fe, and Cu and therefore has attracted the interest of producers and breeders (Wiwart et al, 2013 ). Meanwhile, dwarf polish wheat (DPW), collected from Tulufan, Xingjiang, China, shows high tolerance to Cd and Zn because its growth is not affected by the accumulation of high concentrations of these metals in seedlings (Wang X. et al, in press ). However, molecular responses to Cd and Zn remain unknown.…”

Section: Introductionmentioning

confidence: 99%

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Proteomic Profiling of the Interactions of Cd/Zn in the Roots of Dwarf Polish Wheat (Triticum polonicum L.)

Wang

et al. 2016

Front. Plant Sci.

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Cd and Zn have been shown to interact antagonistically or synergistically in various plants. In the present study of dwarf polish wheat (DPW)roots, Cd uptake was inhibited by Zn, and Zn uptake was inhibited by Cd, suggesting that Cd and Zn interact antagonistically in this plant. A study of proteomic changes showed that Cd, Zn, and Cd+Zn stresses altered the expression of 206, 303, and 190 proteins respectively. Among these, 53 proteins were altered significantly in response to all these stresses (Cd, Zn, and Cd+Zn), whereas 58, 131, and 47 proteins were altered in response to individual stresses (Cd, Zn, and Cd+Zn, respectively). Sixty-one differentially expressed proteins (DEPs) were induced in response to both Cd and Zn stresses; 33 proteins were induced in response to both Cd and Cd+Zn stresses; and 57 proteins were induced in response to both Zn and Cd+Zn stresses. These results indicate that Cd and Zn induce differential molecular responses, which result in differing interactions of Cd/Zn. A number of proteins that mainly participate in oxidation-reduction and GSH, SAM, and sucrose metabolisms were induced in response to Cd stress, but not Cd+Zn stress. This result indicates that these proteins participate in Zn inhibition of Cd uptake and ultimately cause Zn detoxification of Cd. Meanwhile, a number of proteins that mainly participate in sucrose and organic acid metabolisms and oxidation-reduction were induced in response to Zn stress but not Cd+Zn stress. This result indicates that these proteins participate in Cd inhibition of Zn uptake and ultimately cause the Cd detoxification of Zn. Other proteins induced in response to Cd, Zn, or Cd+Zn stress, participate in ribosome biogenesis, DNA metabolism, and protein folding/modification and may also participate in the differential defense mechanisms.

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Section: Discussionmentioning

confidence: 75%

Section: Introductionmentioning

confidence: 99%

Proteomic Profiling of the Interactions of Cd/Zn in the Roots of Dwarf Polish Wheat (Triticum polonicum L.)

Wang

et al. 2016

Front. Plant Sci.

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“…GSH marked xenobiotic molecules are transported via phase III proteins such as ABC transporters. The ABC transporters comprise large protein families that mediate various physiological processes [55]. In ssion yeast, Cd forms complexes with either GSH or PCs and subsequently transports into vacuoles via the ABC transporters.…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptome analysis uncovers roles of hydrogen sulfide for alleviating cadmium toxicity in Tetrahymena thermophila

Xu²,

et al. 2020

Preprint

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Background: Cadmium (Cd) is a nonessential heavy metal with potentially deleterious effects on different organisms. The organisms have evolved sophisticated defense system to alleviate heavy metal toxicity. Hydrogen sulfide (H2S) effectively alleviates heavy metal toxicity in plants and reduces oxidative stress in mammals. However, the function of H2S for alleviating heavy metal toxicity in aquatic organisms remains less clear. Tetrahymena thermophila is an important model organism to evaluate toxic contaminants in an aquatic environment. In this study, the molecular roles of exogenously H2S application were explored by RNA sequencing under Cd stress in T. thermophila.Results: The exposure of 30 µM Cd resulted in T. thermophila growth inhibition, cell nigrescence, and malondialdehyde (MDA) content considerably increase. However, exogenous NaHS (donor of H2S, 70 µM) significantly alleviated the Cd-induced toxicity by inhibiting Cd absorbtion, promoting CdS nanoparticles formation and improved antioxidant system. Comparative transcriptome analysis showed that the expression levels of 9152 genes changed under Cd stress (4658 upregulated and 4494 downregulated). However, only 1359 genes were differentially expressed with NaHStreatment under Cd stress (1087 upregulated and 272 downregulated). The functional categories of the differentially expressed genes (DEGs) by gene ontology (GO) revealed that the transcripts involved in the oxidation–reduction process, oxidoreductase activity, glutathione peroxidase activity, and cell redox homeostasis were the considerable enrichments between Cd stress and NaHS treatment under Cd stress. Kyoto Encyclopedia of Genes and Genomes (KEGG) indicated that the carbon metabolism, glutathione metabolism, metabolism of xenobiotics by cytochrome P450, and ABC transporters were significantly differentially expressed components between Cd stress and NaHS treatment under Cd stress in T. thermophila. The relative expression levels of six DEGs were further confirmed through quantitative real-time polymerase chain reaction (qRT-PCR). Conclusion: NaHS alleviated Cd stress mainly through inhibiting Cd absorbtion, promoting CdS nanoparticles formation, increasing oxidation resistance, enhancing detoxification, and regulation of transport in free-living unicellular T. thermophila. These findings will expand our understanding for H2S functions in the freshwater protozoa.

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“…Owing to their metal transportation capacity ABC C-type transporters AtABCC1 and AtABCC2 have been identified as major phytochelatin-heavy metal(oid) complex transporters. In recent study in wheat, expression of 13 ABC transporter genes was analyzed in different metals, which suggested that these genes were differentially regulated by Cd, indicating their participation in Cd uptake, transport, and sequestration (Wang X. et al, 2017 ). Yet another transporter of ABC family named as MRP1 (multidrug associated protein) is known to be regulated in ERK/MAPK pathway dependent manner in leukemic T cells (El Azreq et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Traversing the Links between Heavy Metal Stress and Plant Signaling

et al. 2018

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Plants confront multifarious environmental stresses widely divided into abiotic and biotic stresses, of which heavy metal stress represents one of the most damaging abiotic stresses. Heavy metals cause toxicity by targeting crucial molecules and vital processes in the plant cell. One of the approaches by which heavy metals act in plants is by over production of reactive oxygen species (ROS) either directly or indirectly. Plants act against such overdose of metal in the environment by boosting the defense responses like metal chelation, sequestration into vacuole, regulation of metal intake by transporters, and intensification of antioxidative mechanisms. This response shown by plants is the result of intricate signaling networks functioning in the cell in order to transmit the extracellular stimuli into an intracellular response. The crucial signaling components involved are calcium signaling, hormone signaling, and mitogen activated protein kinase (MAPK) signaling that are discussed in this review. Apart from signaling components other regulators like microRNAs and transcription factors also have a major contribution in regulating heavy metal stress. This review demonstrates the key role of MAPKs in synchronously controlling the other signaling components and regulators in metal stress. Further, attempts have been made to focus on metal transporters and chelators that are regulated by MAPK signaling.

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Transcriptome-wide identification and expression analyses of ABC transporters in dwarf polish wheat under metal stresses

Cited by 29 publications

References 57 publications

Proteomic Profiling of the Interactions of Cd/Zn in the Roots of Dwarf Polish Wheat (Triticum polonicum L.)

Proteomic Profiling of the Interactions of Cd/Zn in the Roots of Dwarf Polish Wheat (Triticum polonicum L.)

Comparative transcriptome analysis uncovers roles of hydrogen sulfide for alleviating cadmium toxicity in Tetrahymena thermophila

Traversing the Links between Heavy Metal Stress and Plant Signaling

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