Transcriptome-wide characterization of the WRKY family genes in Lonicera macranthoides and the role of LmWRKY16 in plant senescence

Cao, Zhengyan; Wu, Peiyin; Gao, Hongmei; Xia, Ning; Jiang, Ying; Li, Guohua; Chen, Zexiong

doi:10.1007/s13258-021-01118-8

Cited by 4 publications

(1 citation statement)

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“…3). In accordance with the classi cation of WRKYs in other plant species, especially Arabidopsis, Glycyrrhiza glabra and Lonicera macranthoides [32,33], Group was further divided into ve subgroups. An evolution analysis in group showed that subgroup IIa and IIb were closely related, and the same went between IId and IIe (Fig.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome-wide characterization of novel WRKY family genes Engaged in Crocin biosynthesis in Crocus Sativus

Gao,

Li,

Wang

et al. 2024

Preprint

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Crocins, exhibiting remarkable pharmacological value were generated in significant quantities in stigma of Crocus sativus. Although the biosynthesis pathway of crocins has been elucidated to a great degree, there is still little information about the regulating mechanism of TFs on the biosynthesis of crocins in stigma development process. WRKY TFs were reported to play a role in modulating carotenoids/apocarotenoids metabolism. CsWRKY genes were identified from RNA sequencing database of stigma at different developmental stages. Phylogenetic analysis was employed to work out their evolutionary relation. Coexpression profile of CsWRKY genes and crocin biosynthesis-related genes was performed on Majorbio Cloud Platform. Quantitative real-time PCR was used to investigate the expression level of CsWRKY TFs in various tissue and developmental stages. A total of 34 CsWRKY TFs were identified from stigma of C. Sativus. Phylogenetic analysis of these CsWRKY TFs together with their orthologs from Arabidopsis clustered them into group Ⅰ, Ⅱ and Ⅲ. Coexpression network between CsWRKY TFs and crocin biosynthesis-related genes (CsBCH, CsCCD2L, CsALDH and CsUGT) revealed that CsWRKY1, -2, -8, -10, -15 and − 28 genes showed strong correlation with above structure genes. CsWRKY2, -15 and − 28 have identical motifs, belonging to group Ⅱd. The expression levels of candidate CsWRKY genes are highest in stigma comparing with other tissues. Furtherly, the expression patterns of candidate CsWRKY genes were in parallel to the accumulation of crocins. Our study established an extensive overview of the WRKY family in C. sativus and screened six candidate CsWRKY (1, -2, -8, -10, -15 and − 28) involved in the crocin biosynthesis in saffron.

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Section: Discussionmentioning

confidence: 99%